Unimicro Technologies EasySep-1050 Semi-Preparative HPLC System

Overview

The Unimicro Technologies EasySep-1050 Semi-Preparative HPLC System is a robust, high-pressure liquid chromatography platform engineered for analytical-to-semi-preparative scale separation tasks in research laboratories, quality control environments, and method development workflows. Built upon classical reversed-phase, normal-phase, and ion-exchange chromatographic principles, the system delivers precise gradient elution, reproducible retention time stability, and scalable fraction collection capability. Its architecture conforms to core ISO/IEC 17025-aligned operational expectations for chromatographic instrumentation—particularly in terms of pressure integrity, flow accuracy, and detector linearity—making it suitable for applications requiring trace-level quantification, purity assessment, and small-molecule purification under non-GMP but GLP-compliant conditions.

Key Features

• High-pressure binary pump with floating plunger assembly design, ensuring coaxial alignment between plunger rod and seal to extend service life and reduce maintenance frequency.
• Short-stroke cam-driven plunger mechanism minimizing pulsation (< 0.2 MPa), critical for baseline stability during high-sensitivity detection and low-volume fraction collection.
• Microprocessor-controlled microstepping motor drive delivering smooth, low-noise operation and fine flow resolution (0.01 mL/min increments across 0.01–49.99 mL/min range).
• Dual-wavelength UV-Vis detector featuring deuterium lamp source, precision optical path alignment, and direct digital signal output—eliminating analog-to-digital conversion artifacts and reducing baseline noise to ≤ ±1 × 10⁻⁵ AU (MeOH, 1 mL/min, 254 nm, 1 s).
• Integrated column oven with programmable temperature control (10–60 °C) supporting method robustness and retention time reproducibility across seasonal ambient fluctuations.
• 120-position autosampler with 1–50 µL injection volume flexibility, compatible with standard 2 mL vials and enabling unattended overnight runs for method screening or batch analysis.

Sample Compatibility & Compliance

The EasySep-1050 supports a broad spectrum of analytes including pharmaceutical intermediates, natural product extracts, synthetic oligomers, peptides, and small organic molecules. It accommodates common stationary phases (C18, C8, phenyl-hexyl, silica, amino, and cyano columns) with internal diameters ranging from 10 mm to 30 mm and lengths up to 250 mm. While not certified for 21 CFR Part 11 compliance out-of-the-box, its RS-232 serial interface enables integration into validated LIMS or ELN ecosystems where audit trails, electronic signatures, and data integrity controls are externally managed. The system meets ASTM D7243 (HPLC system suitability testing) and ISO 17025 clause 6.4.3 requirements for equipment verification, including flow accuracy (±2%), flow precision (< 0.2% RSD), wavelength accuracy (±1 nm), and photometric linearity over 0–2 AU.

Software & Data Management

Control and data acquisition are performed via Unimicro’s proprietary EasyChrom software, supporting real-time chromatogram visualization, peak integration using tangent skim and valley-to-valley algorithms, and customizable reporting templates compliant with internal QA documentation standards. Raw data files are stored in vendor-neutral .csv and .txt formats, facilitating third-party processing in tools such as Chromeleon, OpenChrom, or MATLAB-based custom scripts. All detector signals—including absorbance, time, and pressure—are timestamped at 100 Hz sampling rate, ensuring sufficient temporal resolution for kinetic studies or fast-eluting peaks. Audit trail functionality (user login, method change logs, injection history) is enabled and exportable for internal review or regulatory pre-audit preparation.

Applications

• Purification of reaction mixtures in medicinal chemistry labs prior to NMR or MS characterization.
• Stability-indicating assay development for API degradation products per ICH Q1–Q5 guidelines.
• Isolation of bioactive compounds from plant extracts for subsequent pharmacological evaluation.
• Method transfer from analytical to semi-preparative scale using retention time normalization and linear velocity scaling.
• Supporting QC release testing of excipients where impurity profiling demands higher loading capacity than analytical HPLC allows.

FAQ

Is the EasySep-1050 compatible with mass spectrometry interfaces?

Yes—its low-flow compatibility (down to 0.01 mL/min) and modular detector bay allow coupling with electrospray ionization (ESI) sources when operated in analytical mode; however, semi-preparative fractions require post-column splitting or fraction re-injection for LC-MS analysis.
Can the system be upgraded to full preparative scale?

No—the EasySep-1050 is mechanically and hydraulically optimized for ≤20 mL/min operation; full preparative systems (e.g., EasySep-2000 series) feature reinforced manifolds, larger-volume pumps, and dedicated fraction collectors.
Does the UV detector support variable wavelength scanning during a run?

Yes—EasyChrom software enables time-programmed wavelength switching across the 190–600 nm range, allowing multi-analyte detection without compromising sensitivity for individual components.
What validation documentation is provided with the instrument?

Each unit ships with Factory Acceptance Test (FAT) report, Certificate of Conformance, and IQ/OQ protocol templates aligned with ISO/IEC 17025 Annex A. Installation and operational qualification support is available upon request.
Is蒸发光散射检测器 (ELSD) supported natively?

The UM3000 and UM5000 ELSD modules are optional accessories; they connect via analog voltage output and are controlled externally—fraction collection timing must be synchronized manually or via TTL trigger signal from the autosampler.