Wufeng EX1600 Advanced Quaternary Gradient High-Performance Liquid Chromatograph
| Brand | Wufeng |
|---|---|
| Origin | Shanghai, China |
| Model | EX1600 |
| Instrument Type | Fast HPLC System |
| Flow Rate Range | 0.001–5.0 mL/min |
| Maximum Pressure | 42 MPa |
| Sample Tray Capacity | 108 positions (2 mL vials) |
| Injection Volume Range | 0.1–120 µL |
| Column Oven Temperature Range | 10–85 °C |
| UV-Vis Wavelength Range | 180–700 nm |
| Data Acquisition Rate | 20 Hz |
Overview
The Wufeng EX1600 is an advanced quaternary gradient high-performance liquid chromatograph engineered for precision, long-term reliability, and regulatory-compliant operation in QC, R&D, and pharmaceutical laboratories. Based on low-pressure mixing architecture with digitally controlled proportional solenoid valves, the system implements a true four-solvent gradient delivery mechanism—A, B, C, and D—enabling highly accurate mobile phase composition across the full range (0.1–100% per channel) even at sub-microliter flow rates. Its microprocessor-driven pump design minimizes pulsation and improves mixing homogeneity, directly enhancing retention time stability and peak shape reproducibility. The integrated UV-Vis detector features a dual-conical flow cell geometry and a high-stability deuterium lamp, delivering baseline noise < ±0.5 × 10⁻⁵ AU at 254 nm and enabling detection limits suitable for trace-level quantitation in complex matrices. Designed for continuous operation under GLP/GMP environments, the EX1600 meets core performance expectations defined in USP , ISO 17025, and ICH Q2(R2) guidelines for method validation.
Key Features
- Quaternary gradient system with real-time digital flow proportioning—eliminates mechanical cam-based inaccuracies common in legacy low-pressure mixers
- Precision piston pump with active pulse damping; flow accuracy ±0.15% RSD at 1.0 mL/min, linearity ≥99.9%
- Column compartment with Peltier-based temperature control (10–85 °C), uniformity ±0.3 °C, and programmable ramp profiles
- UV-Vis detector with spectral scanning capability (180–700 nm), 10 mm pathlength flow cell, and 20 Hz data capture rate for sharp peak resolution
- Autosampler with 108-position 2 mL vial capacity, needle wash station, and programmable injection volume (0.1–120 µL) with ≤0.25% RSD precision
- Fully embedded controller architecture—no external PC required for basic instrument operation or method execution
- Real-time diagnostics via onboard sensors monitoring pressure, temperature, lamp energy, valve position, and motor current
Sample Compatibility & Compliance
The EX1600 supports reversed-phase, normal-phase, ion-exchange, and size-exclusion chromatography applications using standard 2.1–4.6 mm ID columns up to 250 mm length. It is compatible with common HPLC solvents (acetonitrile, methanol, water, buffers pH 2–8) and accommodates column chemistries including C18, C8, phenyl-hexyl, HILIC, and silica. All hardware modules comply with IEC 61010-1 safety standards and electromagnetic compatibility (EMC) requirements per EN 61326-1. The system architecture supports 21 CFR Part 11 compliance when operated with EX1600 WS software configured with audit trail, electronic signatures, and role-based access control—validated for use in FDA-regulated environments per GMP Annex 11 and EU GMP Annex 11 requirements.
Software & Data Management
The EX1600 WS Chromatography Data System (CDS) is a modular, Windows-based platform supporting instrument control, real-time acquisition, peak integration, calibration curve generation, report export (PDF, CSV, XML), and multi-user workflow management. It includes built-in system suitability testing (SST) templates aligned with USP and EP monographs, customizable reporting formats compliant with ALCOA+ principles, and automated recalibration workflows. Audit trail functionality logs all user actions—including method edits, sequence changes, and result overrides—with immutable timestamps and operator identification. Data integrity safeguards include encrypted storage, automatic backup scheduling, and optional integration with LIMS via ASTM E1384 or HL7 interfaces.
Applications
The EX1600 is routinely deployed in pharmaceutical quality control for assay, related substances, and dissolution testing of small-molecule APIs and finished dosage forms. In food safety labs, it enables pesticide residue screening (e.g., multi-residue analysis per SANTE/11312/2021), mycotoxin quantification, and vitamin profiling. Environmental applications include PAHs, phthalates, and perfluorinated compound analysis in water and soil extracts. Its fast gradient capability (≤5 min run times for standard assays) and robust autosampler support high-throughput batch processing in contract research organizations and central analytical labs.
FAQ
Does the EX1600 support method transfer from legacy HPLC systems?
Yes—the system accepts standard .cdf and .u3d method files and includes retention time prediction tools based on solvent strength modeling.
Is column switching supported?
Yes, optional 6-port/2-position and 10-port/2-position switching valves are available with full software integration.
What maintenance intervals are recommended?
Pump seal replacement every 6,000 operating hours; UV lamp replacement every 2,000 hours; annual preventive maintenance including pressure sensor calibration and detector wavelength verification.
Can the EX1600 be integrated into a networked lab environment?
Yes—it features Ethernet (10/100 Mbps) and USB 2.0 connectivity, supports DHCP and static IP assignment, and is compatible with enterprise Active Directory authentication.
Is remote monitoring possible?
Yes—EX1600 WS includes web-enabled status dashboard accessible via secure HTTPS, displaying real-time pressure, temperature, lamp energy, and run queue status.
