Wufeng LC-80 ChroMini High-Performance Liquid Chromatograph
| Brand | Wufeng |
|---|---|
| Origin | Shanghai, China |
| Model | LC-80 |
| Instrument Type | Conventional HPLC System |
| Flow Rate Range | 0.001–5.000 mL/min |
| Maximum Pressure | 40 MPa |
| Injection Mode | Manual |
| Injection Volume Range | 1–100 µL |
| Column Oven Temperature Control | Ambient Only |
| UV-Vis Wavelength Range | 190–680 nm |
| Data Acquisition Frequency | 10 Hz |
| Flow Precision (RSD) | < 0.1% |
| Flow Accuracy (Ss) | ≤ ±1% |
| Retention Time Repeatability (RSD₆) | ≤ ±0.2% (naphthalene/methanol) |
| Peak Area Repeatability (RSD₆) | ≤ ±0.5% (naphthalene/methanol) |
| Pressure Pulsation | < 0.1 MPa |
| Spectral Bandwidth | 8 nm |
| Wavelength Accuracy | ±1 nm |
| Wavelength Precision | < 0.1 nm |
| Baseline Noise | ≤ 0.25 × 10⁻⁵ AU (static) |
| Baseline Drift | ≤ 0.4 × 10⁻⁴ AU/hr (static) |
| LOD (naphthalene/methanol) | ≤ 5 × 10⁻⁹ g/mL |
Overview
The Wufeng LC-80 ChroMini High-Performance Liquid Chromatograph is a compact, modular, and fully integrated HPLC system engineered for precision, operational simplicity, and laboratory flexibility. Based on classical reversed-phase and normal-phase liquid chromatography principles, the LC-80 employs high-pressure solvent delivery, precise manual injection, and dual-beam UV-Vis absorbance detection to separate, identify, and quantify analytes in complex liquid matrices. Its microprocessor-controlled architecture enables deterministic gradient formation, real-time pressure monitoring, and synchronized data acquisition at up to 10 Hz—ensuring robust retention time alignment and peak integrity across routine QC, academic research, and method development workflows. Designed with minimal internal dead volume and optimized fluidic pathways, the system reduces band broadening and improves resolution without requiring extensive plumbing or external controllers.
Key Features
- Integrated pump module with digital flow control delivering 0.001–5.000 mL/min at pressures up to 40 MPa, supporting C18, phenyl-hexyl, HILIC, and ion-exchange columns
- Manual six-port injection valve with reproducible 1–100 µL sample loop capability; compatible with standard 2 mL vials and syringe-based loading
- Fixed-wavelength UV-Vis detector featuring a 190–680 nm spectral range, 8 nm bandwidth, and ±1 nm wavelength accuracy—calibrated against NIST-traceable holmium oxide and didymium filters
- Real-time system diagnostics including leak detection, pump head pressure loss alarm, and over-pressure cutoff—enabling proactive maintenance and instrument protection
- Compact footprint (≤ 450 × 350 × 250 mm) and lightweight chassis (< 25 kg), facilitating benchtop deployment, mobile lab integration, and cleanroom-compatible installation
- Embedded firmware with Ethernet connectivity for remote status polling, parameter logging, and firmware updates via secure HTTP/HTTPS protocols
Sample Compatibility & Compliance
The LC-80 supports aqueous-organic mobile phases (e.g., water/acetonitrile, water/methanol, buffer-based systems) and accommodates standard 3.9–4.6 mm ID analytical columns (50–250 mm length). It meets essential performance criteria outlined in ASTM D7097 (HPLC system suitability), ISO 17025:2017 (method validation requirements), and USP (chromatographic separations). While not pre-certified for 21 CFR Part 11, its workstation software architecture supports audit trail generation, electronic signature integration, and user-access-level configuration—enabling laboratories to implement compliant workflows under GLP or GMP environments when paired with validated SOPs and change control procedures.
Software & Data Management
The LC-80 operates exclusively through Wufeng’s ChroMini Chromatography Workstation—a Windows-based application supporting method building, sequence definition, real-time chromatogram visualization, peak integration (tangent skim, valley-to-valley, baseline correction), and customizable report templates. Raw data are stored in vendor-neutral .cdf format (compatible with OpenChrom and Chromeleon viewers). The software logs all instrument events—including flow rate changes, pressure excursions, lamp on/off cycles, and user login/logout—with timestamped entries retained for ≥18 months. Optional add-ons include batch processing macros, calibration curve fitting (linear, quadratic, weighted), and export to CSV, PDF, or LIMS-ready XML schemas.
Applications
- Routine quality control of pharmaceutical actives and excipients per ICH Q2(R2) guidelines
- Environmental analysis of pesticides, PAHs, and phenolic compounds in water and soil extracts
- Food safety testing for preservatives (e.g., benzoic acid, sorbic acid), artificial colors, and mycotoxins
- Academic teaching labs requiring reliable, low-maintenance HPLC platforms for undergraduate chromatography instruction
- Method scouting and transfer studies where system portability and rapid setup are critical
- Stability-indicating assays for degradation product profiling under forced degradation conditions
FAQ
Is the LC-80 compatible with gradient elution?
Yes—the integrated binary pump supports two-solvent gradient programming with linear, step, or multi-segment profiles, configurable via the ChroMini workstation.
Does the detector support variable wavelength scanning?
No—the LC-80 features a fixed-wavelength UV-Vis detector optimized for single-wavelength quantitation; it does not perform full-spectrum scanning or photodiode array acquisition.
Can the system be upgraded to autosampler functionality?
Not natively—the LC-80 is designed as a manual-injection platform; no mechanical or firmware provisions exist for retrofitting an autosampler module.
What column oven options are available?
None—the LC-80 operates at ambient temperature only; external column ovens or thermostatted compartments must be sourced separately.
Is technical support available outside mainland China?
Wufeng provides documentation in English and offers remote troubleshooting assistance; hardware service contracts and on-site support require regional distributor coordination.
