Thermo Fisher Dionex AS-AP Automated Sample Injector (Used, Refurbished)

Overview

The Thermo Fisher Dionex AS-AP Automated Sample Injector is a high-precision, modular autosampler engineered for ion chromatography (IC) and liquid chromatography (LC) systems. Designed around a robust syringe-driven, moving-needle architecture, it delivers reproducible sample introduction with ≤0.3% relative standard deviation (RSD) across repeated injections—critical for quantitative trace analysis in regulated environments. Its core measurement principle relies on precise volumetric displacement via a PEEK-coated stainless-steel syringe and inert fluidic path, minimizing analyte adsorption, carryover, and matrix interference. The AS-AP integrates seamlessly with Thermo Scientific ICS-series IC systems and compatible third-party LC platforms, supporting both isocratic and gradient elution workflows. Unlike fixed-needle designs, its mobile needle enables adaptive sampling from diverse vessel formats—including 10 mL vials (81-position tray), 96-well and 384-well microplates (up to 1152 samples), and custom tube racks—without hardware reconfiguration.

Key Features

• Mobile-needle sampling mechanism ensures consistent aspiration and dispensing across heterogeneous container geometries (vials, deep-well plates, PCR strips)
• Full PEEK™ fluidic pathway—including needle, rotor seal, and internal tubing—provides exceptional chemical resistance and low background contamination for anion/cation, organic acid, and small-molecule analysis
• Programmable sample tray cooling (4–60°C) maintains thermal integrity of labile analytes (e.g., nitrite, sulfite, amino acids) during extended unattended runs
• Sub-30-second cycle time for 5 µL injections, optimized via rapid syringe refill, valve switching, and needle wash protocols
• Dual-injection mode supports parallel or sequential operation across two independent IC systems, eliminating reagent cross-contamination in multi-method labs
• Integrated pre-injection analytics: automated conductivity and pH measurement prior to injection, enabling real-time sample qualification and method-triggered rejection
• Onboard fraction collection and re-injection capability with programmable volume control and post-collection dilution—essential for offline concentration verification and method development

Sample Compatibility & Compliance

The AS-AP accommodates aqueous, organic-aqueous, and mildly acidic/basic matrices typical in environmental water testing (EPA Methods 300.0, 300.1), pharmaceutical counterion analysis (USP ), food safety (AOAC 971.23), and semiconductor ultrapure water monitoring. Its design conforms to ISO/IEC 17025:2017 requirements for calibration traceability and performance verification. When deployed in GxP-regulated settings, the system supports audit-trail-enabled software (via Chromeleon CDS) compliant with FDA 21 CFR Part 11 for electronic records and signatures. All refurbished units undergo full functional validation per Thermo Fisher service bulletins, including carryover assessment (<0.01%), linearity verification (0.1–100 µg/L), and temperature stability testing across the full cooling range.

Software & Data Management

Controlled exclusively through Thermo Scientific Chromeleon Chromatography Data System (CDS) v7.3 or later, the AS-AP leverages native driver integration for method synchronization, sequence scheduling, and real-time status monitoring. Chromeleon provides GLP-compliant features including user access levels, electronic signatures, change control logs, and raw data immutability. Method templates support automated dilution series, internal standard spiking (pre- or post-column), column-switching sequences, and conditional logic (e.g., “inject only if pH > 5.5”). Raw injection metadata—including syringe pressure, valve actuation timestamps, needle position coordinates, and cooling chamber temperature logs—is embedded in each .cdf file for forensic traceability.

Applications

• Environmental: Quantification of fluoride, chloride, nitrate, sulfate, and perchlorate in drinking water, wastewater, and soil leachates
• Pharmaceutical: Residual catalyst metal analysis (Na⁺, K⁺, Ca²⁺, Mg²⁺) in APIs; counterion profiling of biologics formulations
• Food & Beverage: Organic acid profiling (lactic, acetic, citric) in dairy and fermented products; preservative detection (benzoate, sorbate)
• Electronics: Trace anion monitoring (Cl⁻, F⁻, SO₄²⁻) in ultrapure rinse water per SEMI F57 standards
• Clinical Research: Low-volume serum/urine analysis of iodide, thiocyanate, and selenite using online preconcentration cartridges

FAQ

Is this unit factory-refurbished or dealer-refurbished?
This AS-AP unit has been professionally refurbished by Spectralab Scientific Inc., an ISO 9001-certified laboratory equipment service provider. It includes full mechanical inspection, fluidic path replacement, syringe recalibration, and firmware update to the latest stable release.
What documentation accompanies the instrument?
Each unit ships with a Certificate of Functional Verification, detailed service report, Chromeleon CDS installation media, and original Thermo Fisher operator manuals (digital and printed). A traceable calibration record against NIST-traceable standards is provided.
Can the AS-AP be integrated with non-Thermo LC systems?
Yes—via external TTL or RS-232 triggering, the AS-AP operates in “standalone” mode synchronized with any HPLC or UHPLC system supporting external start signals. Valve timing and injection volume remain fully programmable through Chromeleon.
Does the 6-month warranty cover parts, labor, and on-site service?
The warranty covers all parts and labor for defects in materials or workmanship. Remote diagnostics and software support are included; on-site service is available at additional cost within North America and Europe.
Are spare consumables (needles, rotors, seals) available for immediate shipment?
Yes—Spectralab maintains inventory of genuine Thermo Fisher AS-AP consumables, including PEEK needles (P/N 074375), rotor seals (P/N 074376), and syringe assemblies (P/N 074377), all shipped with certified lot documentation.