Agilent 1200 Infinity Series Preparative Liquid Chromatograph with Fraction Collector

Overview

The Agilent 1200 Infinity Series Preparative Liquid Chromatograph with Fraction Collector is a field-proven, modular high-performance liquid chromatography platform engineered for robust, reproducible purification of small- to medium-scale compounds. Built upon Agilent’s established Infinity architecture, this refurbished preparative system employs low-dispersion, high-pressure gradient pumping (up to 400 bar), precise flow control (0.01–100 mL/min), and real-time UV-Vis detection across dual G1315B diode array detectors. The integrated G1364B preparative fraction collector enables time-, peak-, or threshold-triggered collection into standard microtiter plates, test tubes, or custom vial racks — critical for compound isolation in synthetic chemistry, natural product purification, and early-stage pharmaceutical process development. Unlike analytical-scale systems, this configuration prioritizes mass recovery, column loading capacity (up to 30 mm ID columns), and solvent compatibility with aggressive mobile phases (e.g., THF, chloroform, DMSO). Its hardware design conforms to ISO 9001–certified refurbishment protocols, including full pressure testing, pump seal replacement, detector lamp calibration, and baseline noise verification per ASTM E260–21.

Key Features

• Quad-channel preparative pumping (G1361A): Independent flow control per channel; gradient mixing accuracy ±0.5% RSD; pulse dampening optimized for >5 mL/min flow stability
• Dual G2260A preparative autosamplers: 100-position vial capacity per unit; injection volume range 0.1–5 mL; needle wash integration with dual solvent paths to prevent cross-contamination
• Dual G1315B UV-Vis detectors: Variable wavelength scanning (190–950 nm); 10 mm pathlength flow cells; linear dynamic range >2.5 AU for accurate peak quantitation during fractionation
• Dual G1364B fraction collectors: Positional accuracy ±0.2 mm; programmable trigger logic (UV threshold, time window, or derivative-based peak apex); compatibility with 96-well plates, 13×100 mm tubes, and custom trays
• Refurbishment validation: Includes full system leak testing at maximum operating pressure, pump flow rate verification across 5–100 mL/min, detector linearity assessment per NIST-traceable standards, and firmware updated to latest stable revision (A.08.04 or later)

Sample Compatibility & Compliance

This system accommodates diverse sample matrices—including crude reaction mixtures, botanical extracts, peptide digests, and polymer fractions—without requiring extensive pre-filtration, thanks to its 5 µm inlet filter design and high-tolerance check valves. Column compatibility spans reversed-phase (C18, C8), normal-phase (silica, cyano), and size-exclusion media up to 30 mm internal diameter and 250 mm length. From a regulatory perspective, the hardware meets mechanical requirements outlined in USP , EP 2.2.46, and ISO 17025:2017 for chromatographic equipment qualification. When operated with Agilent OpenLab CDS (v2.5+) under validated configurations, the system supports audit trail generation, electronic signatures, and data integrity controls aligned with FDA 21 CFR Part 11 and EU Annex 11 expectations. All refurbished units undergo IQ/OQ documentation review prior to shipment.

Software & Data Management

The Agilent 1200 Infinity platform operates natively with OpenLab CDS, enabling method transfer from analytical to preparative scales via retention time normalization and gradient scaling algorithms. Raw data files (.D format) are stored in hierarchical project structures with embedded metadata (operator ID, timestamp, instrument ID, method version). Batch processing supports automated fraction table generation, UV spectral deconvolution (for co-eluting peaks), and integration with LIMS via ASTM E1384-compliant export templates. All refurbished systems ship with licensed OpenLab CDS Basic Edition; upgrade paths to CDS Advanced (with sequence scheduling, purity analysis, and fraction tracking dashboards) are available upon request. Data backups follow ISO/IEC 27001–aligned retention policies—minimum 30-day local archive plus optional encrypted cloud sync.

Applications

• Synthetic organic chemistry: Isolation of intermediates and final products from Pd-catalyzed couplings, asymmetric reductions, and heterocycle syntheses
• Natural products research: Fractionation of plant extracts for bioactivity-guided isolation (e.g., antimicrobial or kinase inhibition assays)
• Pharmaceutical development: Purification of impurities for structural elucidation (NMR, HRMS) and reference standard preparation per ICH Q5B guidelines
• Peptide & oligonucleotide purification: Desalting and separation of crude solid-phase synthesis products using ion-pairing RP-HPLC
• Materials science: Separation of polymer fractions by molecular weight (SEC mode) or end-group functionality (gradient RP)

FAQ

What documentation accompanies the refurbished system?
Full refurbishment report (including pressure test logs, flow verification charts, detector noise spectra), calibrated certificate for UV lamp intensity, and serialized component traceability list. IQ/OQ templates are provided for customer-executed qualification.
Can this system be upgraded to include mass-directed fractionation?
Yes — the G1364B collector supports external TTL triggering; integration with Agilent 6100 Series single-quadrupole MS (via G1316C column oven interface) enables real-time m/z-triggered collection.
Is method transfer support included?
Standard package includes one remote session with an applications specialist for analytical-to-prep method scaling and fraction collection parameter optimization.
What maintenance contracts are available post-warranty?
Annual Preventive Maintenance Plans (PMPs) cover quarterly performance verification, consumables replenishment (seals, frits, lamps), and priority remote diagnostics — available in 1–3 year terms.
Are spare parts stocked for immediate dispatch?
Yes — all critical spares (G1361A pump heads, G1364B collector syringes, G1315B flow cell windows) are held in regional warehouses and shipped within 24 business hours on confirmed order.