Thermo Fisher TSP & Waters Used HPLC Systems – UV Detectors, Pumps, Autosamplers

Overview

This listing represents a curated inventory of pre-owned, functionally verified high-performance liquid chromatography (HPLC) components sourced from decommissioned Thermo Fisher Scientific TSP and Waters systems. These units were originally deployed in regulated laboratory environments—including QC labs, academic research facilities, and contract testing organizations—and have undergone comprehensive operational validation prior to resale. Each component operates on the fundamental principle of liquid-phase separation: analytes are partitioned between a mobile phase (eluent) and a stationary phase (column packing), with detection achieved via absorbance (UV-Vis), fluorescence emission (FLR), or other physicochemical responses. The TSP UV-150 and FI-2000 detectors employ dual-beam optical architectures for baseline stability; the Waters UV-486 utilizes a fixed-wavelength photodiode array design optimized for routine quantitation. All pumps—TSP P-100 and Waters 510—are reciprocating piston types engineered for pulse-dampened flow delivery at pressures up to 5000 psi. These modules are fully compatible with standard 1/16″ stainless steel or PEEK tubing, 4.6 mm ID analytical columns, and common C18, phenyl-hexyl, or HILIC stationary phases.

Key Features

• Factory-calibrated UV detectors (TSP UV-150: 190–700 nm range; Waters UV-486: 190–600 nm, fixed 254 nm default) with <1.5×10⁻⁵ AU noise specification at 254 nm
• Fluorescence detector (TSP FI-2000) featuring variable excitation/emission wavelength selection and low-picoampere detection limit for PAHs, amino acids, and pharmaceutical metabolites
• High-pressure gradient-capable pumps (Waters 510, TSP P-100) delivering flow rates from 0.01 to 10.0 mL/min with ≤0.1% RSD over 24-hour operation
• Autosampler AS-100 supporting 100-position vial capacity, 1–100 µL injection volume range, and programmable needle wash cycles
• All units refurbished under ISO 9001-certified procedures: mechanical inspection, leak testing, electronic diagnostics, firmware verification, and performance qualification using caffeine, uracil, and naphthalene test mixtures
• No proprietary lock-in: fully interoperable with third-party data systems (e.g., Chromeleon, Empower, OpenLab CDS) via standard RS-232 or analog voltage output interfaces

Sample Compatibility & Compliance

These HPLC components support aqueous, organic, and mixed-solvent mobile phases (e.g., water/acetonitrile, water/methanol, ammonium acetate buffers). They comply with method requirements outlined in ASTM D7097 (hydrocarbon analysis), USP (chromatographic separations), and ICH Q2(R2) (analytical method validation). While not supplied with full GMP documentation packages, each unit bears traceable service logs and calibration history. For laboratories operating under GLP or FDA 21 CFR Part 11 compliance mandates, Spectralab provides optional IQ/OQ documentation templates and on-site installation qualification support upon request.

Software & Data Management

No proprietary software is bundled with these instruments. All detectors and pumps generate analog outputs (0–1 V or 0–5 V) and accept TTL-level start/stop triggers, enabling seamless integration into existing chromatography data systems (CDS). Digital communication occurs via RS-232 serial interface (9600 baud, 8N1), supporting basic command sets for wavelength setting (UV), gain adjustment (FLR), flow rate control (pump), and injection initiation (autosampler). Raw signal data is acquired and processed using validated CDS platforms compliant with audit trail and electronic signature requirements per 21 CFR Part 11 Annex 11 guidelines.

Applications

• Pharmaceutical QC: assay of active pharmaceutical ingredients (APIs), related substances testing, dissolution profile analysis
• Environmental monitoring: pesticide residue screening in soil/water extracts per EPA Method 8081B and 8330B
• Food safety: mycotoxin (aflatoxin B1, ochratoxin A) quantification in cereals and nuts
• Academic research: natural product isolation, polymer molecular weight distribution (GPC), chiral separation using polysaccharide-based columns
• Method development labs: modular configuration allows rapid recombination of detectors, pumps, and injectors for orthogonal detection strategies

FAQ

Are these units covered by manufacturer warranty?
No. As certified pre-owned equipment, all units are sold “as-is, where-is” with no extended warranty. However, each undergoes a 72-hour functional burn-in test prior to shipment.
Can I integrate a TSP UV-150 detector with a Waters pump?
Yes. Both use industry-standard analog voltage output (1 mV/AU) and TTL trigger inputs, enabling cross-platform compatibility without protocol translation.
Do you provide column compatibility guidance?
Yes. Our technical team supplies detailed column coupling recommendations—including recommended guard columns, frit specifications, and pressure drop calculations—for common reversed-phase, HILIC, and ion-exchange chemistries.
Is remote diagnostic support available post-purchase?
Yes. Spectralab offers paid remote troubleshooting sessions (via TeamViewer or AnyDesk) with certified HPLC field engineers experienced in Thermo Fisher and Waters legacy platforms.
What documentation accompanies shipment?
Each unit ships with a Certificate of Functional Verification, service log summary, and mechanical/electrical schematic excerpts. Full OEM manuals are available upon request.