HYSMB6-500 Benchtop Simulated Moving Bed (SMB) Chromatography System
| Origin | Beijing, China |
|---|---|
| Manufacturer Type | Authorized Distributor |
| Origin Category | Domestic |
| Model | HYSMB6-500 |
| Application Level | Laboratory-Scale |
| Instrument Type | High-Pressure Preparative Liquid Chromatograph |
| Flow Rate Range | 0.01–49.99 mL/min |
| Flow Accuracy | <0.5% |
| Flow Precision (RSD) | ≤0.2% |
| Maximum Operating Pressure | 8 MPa |
| Wavelength Range | ±1 nm |
| Wavelength Repeatability | 0.4 nm |
| Baseline Noise | 2×10⁻⁵ AU (254 nm, 1 mL/min, methanol) |
| Data Acquisition Frequency | 5 Hz |
| Column Configuration | 8-column rotary valve system |
| Column Volume | 500 mL per column |
| Feed Flow Range | 0.5–10 mL/min |
| Eluent Flow Range | 0.5–15 mL/min |
| Product Flow Range | 0.1–30 mL/min |
| System Voltage | 220 V ±10%, 50 Hz |
| Total Power Consumption | 2 kW |
| Dimensions (W×D×H) | 450 × 450 × 800 mm |
| Weight | 3.0 kg |
| Communication Interface | RS-485 for external control and SCADA integration (e.g., KingView) |
Overview
The HYSMB6-500 Benchtop Simulated Moving Bed (SMB) Chromatography System is an engineered solution for continuous, high-resolution chiral and isomeric separation under preparative liquid chromatography conditions. Unlike conventional batch-mode column chromatography, SMB technology emulates the countercurrent movement of stationary and mobile phases through a cyclic, multi-column configuration—typically eight columns interconnected via a high-precision rotary distribution valve. This architecture enables simultaneous adsorption, desorption, regeneration, and washing steps across discrete zones, resulting in continuous feed introduction and fractionated product withdrawal with enhanced mass transfer efficiency and reduced solvent consumption. The system operates on the principle of differential affinity kinetics between closely related analytes (e.g., enantiomers, structural isomers, or diastereomers), leveraging thermodynamic selectivity and kinetic resolution under controlled pressure (up to 8 MPa) and flow dynamics. Designed for laboratory-scale process development, the HYSMB6-500 supports method scouting, parameter optimization, and scalability assessment toward pilot or industrial SMB deployment—particularly where traditional batch purification fails to meet purity, yield, or cost targets.
Key Features
- Eight-column SMB architecture with synchronized rotary valve actuation, enabling true continuous operation with defined Zone I–IV functionality (desorb, extract, raffinate, purge)
- High-accuracy dual-pump fluid delivery system: main high-pressure pump (0.01–49.99 mL/min, <0.5% accuracy) and auxiliary micro-dosing pumps (0.1–30 mL/min, 24 V DC) for precise feed/eluent/product stream control
- Integrated UV-Vis detector with ±1 nm wavelength range, 0.4 nm repeatability, and low baseline noise (2×10⁻⁵ AU at 254 nm), optimized for real-time fraction monitoring in methanol- or acetonitrile-based mobile phases
- Tactile 7-inch industrial touchscreen HMI for intuitive parameter setting, zone timing adjustment, pressure/flow logging, and alarm management
- Modular column design: interchangeable stainless-steel columns (500 mL volume each), compatible with particle sizes from 20–40 µm silica-, polymer-, or chiral stationary phases
- RS-485 serial interface supporting bidirectional communication with SCADA systems (e.g., KingView) and third-party LIMS or automation platforms for GLP-compliant audit trails
- Compact benchtop footprint (450 × 450 × 800 mm) with integrated pressure regulation, backpressure control, and leak detection circuitry
Sample Compatibility & Compliance
The HYSMB6-500 accommodates a broad spectrum of thermally stable, soluble compounds amenable to liquid-phase chromatographic separation—including small-molecule pharmaceuticals, natural product isolates, amino acids, sugars, organic acids, and specialty chemicals. It supports both reversed-phase and normal-phase chemistries using commercially available C18, phenyl-hexyl, cellulose-, amylose-, or macrocyclic glycopeptide-based chiral media. System hardware complies with IEC 61000-6-2 (EMC immunity) and IEC 61010-1 (safety requirements for electrical equipment). While not certified for GMP manufacturing, its data integrity features—including timestamped event logs, user-access levels, and configurable audit trail export—align with FDA 21 CFR Part 11 expectations for laboratory-scale process development documentation. Method validation may be performed in accordance with ICH Q2(R2) and ASTM D7976 for preparative chromatographic performance qualification.
Software & Data Management
The embedded firmware provides real-time visualization of pressure profiles, flow rates, UV absorbance trends, and valve position status across all eight columns. All operational parameters are stored locally with time-stamped metadata and can be exported in CSV format for offline analysis. The RS-485 interface allows integration into centralized lab informatics environments; SCADA-level supervision supports remote start/stop commands, setpoint overrides, and fault condition reporting. For regulatory traceability, operator login credentials, parameter changes, and system alarms are recorded with immutable timestamps—enabling reconstruction of experimental conditions during technical transfer or quality review. No proprietary cloud dependency: all data remains on-device unless explicitly exported by authorized users.
Applications
- Pharmaceutical R&D: Enantiomeric resolution of chiral APIs (e.g., β-blockers, NSAIDs), separation of positional isomers (e.g., ortho/meta/para-xylene), and purification of peptide intermediates
- Biotechnology: Continuous isolation of monoclonal antibody fragments, nucleotide analogs, and post-translational modification variants
- Food & Flavor Chemistry: Fractionation of unsaturated fatty acid esters (e.g., EPA/DHA), terpene isomers (limonene/camphene), and Maillard reaction products
- Fine Chemical Synthesis: Recovery of catalyst residues, separation of regioisomeric sulfonation products, and purification of asymmetric synthesis outputs
- Bioresource Processing: Continuous extraction of polyphenols from plant matrices, separation of sugar alcohols (e.g., sorbitol/mannitol), and recovery of high-value alkaloids
FAQ
What is the maximum column packing pressure supported by the HYSMB6-500?
The system is rated for continuous operation up to 8 MPa, with pressure transducers calibrated and validated over the full 0–8 MPa range.
Can the system be operated without the rotary valve for conventional batch chromatography?
No—the HYSMB6-500 is purpose-built for simulated moving bed operation; it does not support single-column gradient elution or isocratic batch runs.
Is method transfer from analytical HPLC to this SMB system feasible?
Yes, provided retention time, selectivity (α), and capacity factor (k’) data are obtained under identical mobile phase and temperature conditions; SMB modeling software (e.g., SMB-Sim, ChromX) is recommended for zone parameter translation.
Does the system include compliance documentation for ISO 9001 or CE marking?
The unit carries CE marking per Machinery Directive 2006/42/EC and EMC Directive 2014/30/EU; ISO 9001 certification applies to the distributor’s quality management system—not individual instruments.
What maintenance intervals are recommended for the rotary distribution valve?
Valve maintenance (seal inspection, rotor cleaning, bearing lubrication) is advised every 500 operational hours or biannually—whichever occurs first—using manufacturer-supplied service kits and torque-calibrated tools.
