DHP-500S Electric Constant Temperature Incubator

Overview

The DHP-500S Electric Constant Temperature Incubator is a precision-engineered, convection-assisted thermal environment system designed for stable, uniform temperature control in life science laboratories. It operates on resistive heating elements coupled with proportional-integral-derivative (PID) temperature regulation—ensuring minimal thermal deviation across the entire chamber volume. Unlike static incubators, the DHP-500S integrates a low-turbulence micro-circulation fan system that actively redistributes heated air, mitigating stratification and achieving ±0.5 °C temperature uniformity (measured at 37 °C per ISO 15189 Annex B methodology). This architecture supports reproducible microbial growth kinetics, cell culture maintenance, enzyme activity assays, and stability testing under controlled thermal conditions—critical for QC/QA workflows in food safety labs, agricultural biotechnology centers, and clinical diagnostics facilities.

Key Features

• PID-based digital temperature controller with high-resolution LCD display and real-time setpoint adjustment (range: ambient +5 °C to 65 °C, resolution: 0.1 °C)
• Double-layer tempered glass observation window integrated into the front door—providing optical clarity without compromising thermal integrity or requiring door opening during monitoring
• High-compliance sealing system using medical-grade silicone rubber gasket and magnetic closure—ensuring >95% chamber integrity retention over 10,000 operational cycles (tested per ASTM F2096)
• Internally mounted axial fan with adjustable airflow profile—optimized to minimize vibration transfer to culture vessels while maintaining laminar recirculation
• Galvanized steel interior chamber with rounded corners and seamless welds—facilitating decontamination via ethanol wipe-down or hydrogen peroxide vapor (HPV) protocols
• Over-temperature protection circuit with independent mechanical cut-off (bimetallic thermostat), compliant with EN 60335-1 Class II appliance requirements

Sample Compatibility & Compliance

The DHP-500S accommodates standard microbiological and cell culture formats including Petri dishes (up to 150 mm diameter), multi-well plates (6–96-well), Erlenmeyer flasks (up to 2 L), and test tube racks. Its chamber geometry allows vertical stacking of up to four standard-height shelf levels (adjustable in 25 mm increments). The unit meets essential regulatory prerequisites for non-GMP laboratory environments: IEC 61010-1 (electrical safety), IEC 61326-1 (EMC immunity), and ISO 14644-1 Class 8 cleanroom compatibility when operated in filtered air environments. While not certified for ISO/IEC 17025 accredited calibration, it supports traceable temperature verification using NIST-traceable PT100 probes (optional accessory). Documentation includes factory calibration certificate and conformity declaration for EU market placement.

Software & Data Management

The DHP-500S operates as a standalone instrument with embedded firmware—no external PC dependency required for routine operation. All temperature logs (setpoint, actual chamber reading, alarm events) are stored internally for up to 30 days at 1-minute intervals. Data export is supported via USB-A port (type-B host interface) using FAT32-formatted flash drives; exported CSV files include timestamp (YYYY-MM-DD HH:MM:SS), setpoint (°C), measured temperature (°C), and status flags (e.g., “HEATING”, “ALERT_OVERTEMP”). Optional RS-485 Modbus RTU interface enables integration into centralized building management systems (BMS) or LIMS platforms for automated audit trail generation—fully compatible with 21 CFR Part 11 electronic record requirements when paired with validated third-party software.

Applications

• Bacterial colony enumeration (ISO 4833-1, AOAC 977.27) and fungal growth assessment (ISO 21527-1)
• Yeast propagation and fermentation starter preparation in food & beverage QA labs
• Seed germination studies and plant tissue culture pre-incubation (ISTA guidelines)
• Enzyme thermal stability profiling (e.g., protease, amylase) across defined temperature gradients
• Short-term storage of diagnostic reagents requiring ambient +10 °C stabilization
• Pre-conditioning of polymer-based biosensors prior to electrochemical characterization

FAQ

What is the temperature recovery time after door opening?
Measured at 37 °C with 15-second door exposure (per ISO 15189 Annex B), recovery to ±0.5 °C deviation requires ≤4.2 minutes.
Can the DHP-500S be used for CO₂-independent cell culture?
Yes—intended for non-CO₂-dependent applications such as bacterial cultures, insect cell lines (e.g., Sf9), and certain primary mammalian cells maintained in buffered media (e.g., HEPES-supplemented DMEM).
Is external humidity control available?
No—the DHP-500S is a dry-air incubator; relative humidity is unregulated and typically stabilizes between 30–50% RH at 37 °C depending on ambient lab conditions.
Does the unit support validation documentation packages?
Factory-installed IQ/OQ documentation templates (in English) are provided upon request; PQ protocol development support is available through authorized service partners.
What maintenance is required for long-term reliability?
Quarterly inspection of fan intake filter (cleanable mesh), annual verification of PID sensor drift (<±0.3 °C), and biannual torque check of door hinge fasteners per maintenance log sheet included in user manual.