Shimadzu LC-10ATvp Used High-Performance Liquid Chromatograph
| Brand | Shimadzu |
|---|---|
| Origin | Japan |
| Model | LC-10ATvp |
| Flow Rate Range | 0.001–9.999 mL/min |
| Maximum Pressure | 40 MPa |
| Flow Precision | ≤0.1% RSD |
| Gradient Accuracy | ±1% (0–100%, water/acetone binary gradient) |
| UV-Vis Detector | SPD-10AVP Plus |
| Wavelength Range | 190–600 nm (SPD-10Avp), 190–900 nm (SPD-10AVvp) |
| Spectral Bandwidth | 8 nm |
| Wavelength Accuracy | ±1 nm |
| Wavelength Repeatability | ±0.1 nm |
| Baseline Drift | ≤2×10⁻⁴ AU/h (250 nm, ambient temperature stable) |
| Noise Level | ≤0.35×10⁻⁵ AU (2 sec time constant, 250 nm) |
| Flow Cell | 10 mm path length, 8 µL volume, 12 MPa max pressure |
| Dimensions (Pump) | W260 × H140 × D420 mm, 11 kg |
| Dimensions (Detector) | W260 × H140 × D420 mm, 13 kg |
| Operating Temperature | 4–35 °C |
| Service History | <2 years in operation |
| Warranty | 6 months post-installation |
Overview
The Shimadzu LC-10ATvp is a pre-owned, high-integrity high-performance liquid chromatography (HPLC) system engineered for precision isocratic and low-pressure binary gradient separations. Built on Shimadzu’s proven VP-series architecture, the LC-10ATvp employs a micro-volume tandem dual-piston pump delivering exceptional flow stability and pulse-free solvent delivery—critical for reproducible retention time alignment and quantitative accuracy across extended analytical runs. Its modular design supports seamless integration with Shimadzu’s SPD-10AVP Plus UV-Vis detector, enabling dual-wavelength monitoring, wavelength scanning, ratio chromatography, and programmable time-based parameter control. The system operates under strict adherence to fundamental chromatographic principles—including Darcy’s law for mobile phase delivery, Beer-Lambert law for absorbance detection, and Van Deemter theory for column efficiency optimization—making it suitable for method development, routine QC, and regulatory-compliant analyses in pharmaceutical, food safety, environmental, and chemical laboratories.
Key Features
- Micro-volume tandem dual-piston pumping mechanism ensuring flow precision of ≤0.1% RSD across the full range (0.001–9.999 mL/min) at pressures up to 40 MPa.
- Enhanced wear resistance via fluoropolymer (Teflon®)-reinforced seals, floating plunger mounting, and corrosion-resistant wetted materials—extending service intervals for consumables including check valves and piston assemblies.
- Onboard maintenance logging and operational history recording accessible via VP key interface—supporting traceability requirements for GLP and GMP environments.
- Integrated safety interlocks during calibration and pressure-limit enforcement to prevent over-pressurization of analytical columns or detector flow cells.
- Scalable configuration: base isocratic pump can be upgraded to low-pressure binary gradient mode by adding auxiliary solvent selection valve (e.g., CBM-10A controller compatibility).
- SPD-10AVP Plus detector featuring dual-lamp source (D₂ + tungsten), broad spectral coverage (190–900 nm), and selectable spectral bandwidth (8 nm), optimized for both UV-sensitive small molecules and visible-range analytes such as dyes or metal complexes.
Sample Compatibility & Compliance
The LC-10ATvp accommodates standard 3.9–4.6 mm ID analytical columns packed with C18, C8, phenyl-hexyl, amino, cyano, or ion-exchange phases. It supports aqueous-organic mobile phases (e.g., water/acetonitrile, water/methanol), buffered systems (e.g., phosphate, acetate), and mildly acidic or basic modifiers—provided pH remains within 2–8 to preserve pump seal integrity and column longevity. The system meets foundational performance criteria outlined in USP , ASTM E260, and ISO 17025 for chromatographic instrument qualification. While not factory-certified for 21 CFR Part 11 compliance, its audit-trail-capable maintenance log and detector parameter programming support retrospective validation documentation when deployed within controlled laboratory information management systems (LIMS) or validated data acquisition platforms.
Software & Data Management
Operation is managed via Shimadzu Class-VP software (v6.x or later), which provides method creation, sequence definition, real-time chromatogram display, peak integration, and report generation. Raw data are stored in proprietary .chd format; export to ASCII (.txt), CSV, or AIA/ANDI-compliant formats enables interoperability with third-party chemometric tools (e.g., MATLAB, SIMCA, OpenChrom). All detector parameters—including wavelength programs, response time settings, auto-zero cycles, and polarity switching—are fully scriptable and time-programmable. Detector firmware supports event-triggered actions (e.g., valve switching upon peak elution), facilitating automated fraction collection or multi-detection workflows. System logs retain timestamps for every hardware initialization, lamp on/off cycle, and manual parameter override—enabling forensic reconstruction of analytical conditions.
Applications
- Pharmaceutical QC: Assay and related substances testing per ICH Q2(R2) guidelines using validated isocratic methods for APIs such as paracetamol, ibuprofen, or metformin.
- Food analysis: Quantification of preservatives (sorbic acid, benzoic acid), sweeteners (aspartame, sucralose), and mycotoxins (aflatoxin B₁) in complex matrices following AOAC or EU Commission Regulation methodologies.
- Environmental monitoring: Determination of priority pollutants—including PAHs, phenols, and chlorinated herbicides—in wastewater and soil extracts per EPA Method 8330B and ISO 14644-1 protocols.
- Chemical R&D: Reaction monitoring of catalytic hydrogenations or esterifications where dual-wavelength detection distinguishes starting material from product based on differential chromophore absorption.
- Academic teaching labs: Robust platform for introducing students to HPLC fundamentals—including retention mechanism interpretation, selectivity tuning, and detector linearity assessment—without requiring enterprise-grade IT infrastructure.
FAQ
Is this unit certified for GMP use?
The LC-10ATvp was originally designed to support GLP/GMP workflows, including maintenance logging and operational traceability. However, formal GMP certification requires site-specific IQ/OQ/PQ validation conducted by qualified personnel using documented protocols—this is the responsibility of the end-user laboratory.
What is included in the 6-month warranty?
The warranty covers functional defects in original Shimadzu pump and detector modules, excluding consumables (lamps, fuses, seals), user-induced damage, or degradation due to improper mobile phase handling (e.g., particulate contamination, pH excursion). Labor and parts replacement are provided onsite or via authorized service depot.
Can the SPD-10AVP Plus perform simultaneous dual-wavelength quantitation?
Yes—the detector supports real-time acquisition at two independently selected wavelengths (e.g., 220 nm and 275 nm) within its specified ranges, with ratio chromatogram output enabled for normalization or interference correction.
Does the system support modern data systems like Empower or Chromeleon?
Native integration is limited to Shimadzu Class-VP. However, ASCII-exported chromatograms and peak tables are compatible with Waters Empower, Thermo Chromeleon, and open-source tools via standardized delimiter formats.
Is column oven compatibility confirmed?
The LC-10ATvp does not include an integrated column heater. It is mechanically and electrically compatible with Shimadzu CTO-10ASvp or third-party Peltier-based ovens via standard 24 V DC trigger and analog temperature feedback interfaces.
