Jiapeng JP-3006 Dual-Beam UV-Vis Nucleic Acid & Protein Detector with Integrated Chromatography System
| Brand | Jiapeng |
|---|---|
| Origin | Shanghai, China |
| Model | JP-3006 |
| Purification Principle | Ion Exchange Chromatography (IEX) |
| Gradient Type | Linear |
| Flow Rate Range | 0.1–10 mL/min |
| Flow Rate Accuracy | ±1.2% |
| Detection Range | −6 AU to +6 AU |
| Maximum Pressure | 3 MPa |
| UV Wavelengths | 260 nm, 280 nm, 295 nm, 310 nm |
| Wavelength Accuracy | ±1 nm |
| Linearity | ±1.5% (0–2 AU) |
| Sample Cell Volume | 10 µL |
| Operating Temperature | 4–40 °C |
| Dimensions (W×D×H) | 345 × 380 × 255 mm |
| Weight | 6.6 kg |
Overview
The Jiapeng JP-3006 Dual-Beam UV-Vis Nucleic Acid & Protein Detector is a compact, integrated chromatography system engineered for precision biomolecule purification and real-time absorbance monitoring in academic, biotech, and QC laboratories. It operates on the principle of dual-beam ultraviolet–visible spectrophotometry, where a reference beam compensates for solvent baseline drift and lamp intensity fluctuations—ensuring high reproducibility in absorbance measurements across extended runs. Designed for versatility in protein and nucleic acid workflows, the JP-3006 supports multiple chromatographic modes including ion exchange chromatography (IEX), affinity chromatography, gel filtration (size exclusion), desalting, and buffer exchange. Its modular architecture enables seamless integration with optional components such as conductivity detectors, manual injectors, and programmable fraction collectors—making it suitable for both method development and routine purification tasks under GLP-aligned operational practices.
Key Features
- Dual-beam optical design with LED cold-light source (260 nm, 280 nm, 295 nm, 310 nm) ensures stable baseline without warm-up delay; instrument achieves equilibrium immediately upon power-on.
- Integrated 12-inch capacitive touchscreen tablet running embedded chromatography software—supports intuitive bilingual interface (English/Chinese) with customizable user profiles and password-protected method access.
- High-accuracy constant-flow peristaltic pump delivering 0.1–10 mL/min flow rates with ±1.2% accuracy across full range; pressure tolerance up to 3 MPa (30 bar) accommodates packed resin columns and high-resolution media.
- Real-time data acquisition and auto-save functionality prevents loss during unexpected power interruption; all chromatograms, method logs, and event timestamps are stored locally with timestamped file naming.
- UV detection range spans −6 AU to +6 AU with linearity maintained at ±1.5% within 0–2 AU—optimized for quantifying low-concentration eluates and detecting trace contaminants.
- 10 µL flow cell volume minimizes sample dispersion while maintaining sensitivity; compatible with standard FPLC-compatible tubing and column fittings (1/16″ OD).
Sample Compatibility & Compliance
The JP-3006 is validated for use with common biological samples including recombinant proteins, monoclonal antibodies, plasmid DNA, oligonucleotides, and viral vectors. Its UV detection channels align with standard analytical benchmarks: 260 nm for nucleic acid quantification (A260), 280 nm for protein estimation (A280), and 295/310 nm for tryptophan-specific monitoring or secondary structure assessment. The system meets essential requirements for ISO 9001-compliant laboratory environments and supports audit-ready documentation when paired with optional electronic signature modules. While not FDA 21 CFR Part 11–certified out-of-the-box, its data export capability (CSV, Excel-compatible .xls) facilitates transfer into validated LIMS or ELN platforms for regulated workflows.
Software & Data Management
The embedded layer chromatography workstation provides synchronized multi-channel visualization: UV absorbance (dual-wavelength overlay), conductivity (when equipped), fraction collection status, and pump control—all rendered on a single screen. Method files include gradient profiles, collection logic (threshold-based, time-based, or peak-triggered), and calibration parameters. All raw data are saved in non-proprietary formats; chromatogram traces can be exported as CSV for third-party analysis (e.g., OriginLab, GraphPad Prism). Audit trail functionality records operator ID, method version, start/stop times, and parameter changes—supporting internal QA reviews and ISO 17025 documentation standards.
Applications
- Purification of His-tagged proteins using Ni-NTA affinity columns with real-time A280/A260 ratio monitoring.
- IEX-based separation of antibody variants (e.g., charge isoforms) under linear salt gradients.
- Gel filtration of protein complexes to assess oligomeric state and aggregation profiles.
- Buffer exchange and desalting of enzymatically digested peptides prior to LC-MS analysis.
- Process validation studies requiring consistent retention time and peak area reproducibility across replicate runs.
FAQ
What wavelengths are available for simultaneous dual-wavelength detection?
The JP-3006 supports fixed-wavelength detection at 260 nm, 280 nm, 295 nm, and 310 nm—selected individually or paired for ratiometric analysis.
Is the system compatible with third-party fraction collectors?
Yes—TTL-level trigger outputs and RS-232 communication ports enable synchronization with external fraction collectors, including models from Gilson, Bio-Rad, and custom-built systems.
Can conductivity detection be added post-purchase?
Yes—optional conductivity detector modules integrate via standard analog input (0–10 V) and are fully configurable within the same software interface.
What is the minimum detectable concentration for BSA at 280 nm?
With a 10 µL flow cell and typical pathlength, the system reliably detects BSA concentrations ≥20 µg/mL (based on ε280 = 43,824 M⁻¹cm⁻¹).
Does the software support method validation protocols (e.g., system suitability tests)?
While not pre-configured for USP/EP pharmacopeial templates, users can define custom pass/fail criteria for peak symmetry, resolution, and RSD of retention time—exportable as PDF reports for internal validation records.
