RWD Passive Anesthesia System for Rodent Surgical Procedures
| Brand | RWD |
|---|---|
| Origin | Shenzhen, China |
| Manufacturer Type | Original Equipment Manufacturer (OEM) |
| Country of Origin | China |
| Model | RWD |
| Pricing | Available Upon Request |
Overview
The RWD Passive Anesthesia System is an engineered solution for precise, low-risk inhalational anesthesia delivery in rodent surgical research. Unlike active vaporizer-based systems requiring continuous gas flow control and calibration, this platform operates on a passive diffusion principle—leveraging calibrated isoflurane or sevoflurane vaporization through a temperature- and humidity-stabilized diffusion chamber. It integrates seamlessly with standard oxygen or medical air supply lines and is designed specifically for consistent induction and maintenance phases in acute surgical interventions. The system meets core functional requirements for GLP-compliant preclinical studies where reproducible anesthetic depth, operator safety, and environmental containment are critical—particularly in neuroscience, cardiovascular, and orthopedic disease modeling workflows.
Key Features
- Passive vaporization architecture eliminates need for electronic flowmeters or pressure-regulated vaporizers—reducing calibration drift and mechanical failure points.
- Dual-stage waste gas scavenging: Integrated activated charcoal filter cartridge (ISO 8573-1 Class 2 compliant) coupled with optional external vacuum-assisted gas recovery unit ensures >99.5% volatile anesthetic capture efficiency per ASTM F2069-22 standards.
- Modular animal restraint platform with adjustable stereotaxic-compatible mounting points, non-slip silicone surface, and integrated thermal regulation interface for compatible warming pads (37 °C ± 0.5 °C stability).
- Standardized face mask assembly with anatomically contoured silicone gasket (available in mouse, rat, and guinea pig sizes) and low-dead-space design (< 5 mL) to minimize rebreathing and accelerate induction kinetics.
- Spill-resistant, autoclavable stainless-steel surgical tray insert with raised edge (15 mm height) and fluid channeling grooves—designed to contain biological fluids and facilitate post-procedure decontamination per ISO 15883-1 guidelines.
Sample Compatibility & Compliance
The system supports mice (15–40 g), rats (100–500 g), and juvenile rabbits (up to 2.5 kg) under controlled ambient conditions (20–24 °C, 40–60% RH). All wetted components comply with USP Class VI biocompatibility testing. Gas filtration media conforms to EN 13544-1:2021 for anesthetic agent adsorption capacity and breakthrough time validation. Device architecture aligns with IEC 61010-1:2010 safety requirements for laboratory equipment. Documentation package includes factory calibration certificates, material traceability records, and conformity declarations supporting FDA 21 CFR Part 11 readiness for electronic record retention in regulated environments.
Software & Data Management
While the core anesthesia delivery module operates without embedded firmware, the optional gas recovery controller (RWD-GRC-200) provides USB-C connectivity and native support for LabVIEW™ 2022 SP1 and MATLAB® R2023a via provided IVI-COM drivers. Real-time exhaust flow rate, filter saturation status (via differential pressure sensor), and ambient isoflurane concentration (ppm) are logged at 1 Hz resolution. Audit trails include user ID, timestamp, parameter changes, and filter replacement events—structured to meet ALCOA+ principles for data integrity in GLP/GMP audits. Export formats include CSV, HDF5, and XML with schema documentation aligned with ISA-88 batch record standards.
Applications
- Transient middle cerebral artery occlusion (tMCAO) and coronary artery ligation models requiring stable surgical plane over extended durations (60–120 min).
- Longitudinal intravital imaging protocols where minimal hemodynamic perturbation during anesthesia maintenance is essential.
- Multi-step orthopedic procedures including ovariectomy-induced osteoporosis model establishment and subsequent micro-CT-guided bone biopsy.
- Cardiovascular interventions such as left ventricular injection, transverse aortic constriction (TAC), and retro-orbital blood sampling under continuous physiological monitoring.
- Terminal tissue harvest protocols—including perfusion fixation—where consistent anesthetic depth directly impacts histomorphometric fidelity.
FAQ
Does this system require external oxygen or compressed air supply?
Yes. A regulated medical-grade gas source (oxygen or medical air) at 30–50 psi is required for operation. Optional integrated pressure regulator (RWD-PR-100) available for direct cylinder connection.
Can the filtration cartridge be regenerated or must it be replaced?
Cartridges are single-use consumables validated for up to 40 hours of cumulative isoflurane exposure at 2.5% concentration. Regeneration is not supported per ISO 15883-5 Annex D.
Is the restraint platform compatible with stereotaxic frames from other manufacturers?
Yes. Mounting holes conform to standard 1/4″-20 UNC thread pattern with 25.4 mm pitch, enabling interoperability with David Kopf, Stoelting, and Leica Biosystems frames.
What validation documentation is provided for regulatory submissions?
Includes Installation Qualification (IQ) and Operational Qualification (OQ) templates, filter breakthrough test reports, and component material certifications—all editable in Word/PDF format for sponsor-specific SOP alignment.
