ROCKER Lafil300 Plus Vacuum Liquid Aspiration System
| Brand | ROCKER |
|---|---|
| Origin | Taiwan |
| Model | Lafil300 Plus |
| Ultimate Vacuum | 105 mbar (–680 mmHg) |
| Maximum Flow Rate | 23 L/min |
| Dimensions (L×W×H) | 35 × 24.3 × 29 cm |
| Pump Type | Oil-Free Piston Vacuum Pump |
| Filtration | 0.2 µm PTFE Disc Filter |
| Waste Bottle Capacity | 1200 mL (PES material) |
| Tip Ejection | Single-Hand Operated Octo-Claw Connector |
| Safety Features | Dual Overflow Protection (Float Valve + In-Line Hydrophobic Filter) |
| Certifications | CE Marked |
| Sterilization Compatibility | Autoclavable Components (121°C, 20 min) |
Overview
The ROCKER Lafil300 Plus Vacuum Liquid Aspiration System is an integrated, oil-free laboratory vacuum solution engineered for precise, contamination-free aspiration of biological liquids—particularly in sensitive cell culture, microbiology, and molecular biology workflows. Unlike conventional vacuum setups requiring separate pumps, traps, and filtration units, the Lafil300 Plus consolidates the vacuum source, waste collection chamber, and ergonomic handheld controller into a single compact platform. Its core operation relies on a maintenance-free oil-free piston pump that generates a stable ultimate vacuum of 105 mbar (–680 mmHg) and delivers a maximum continuous flow rate of 23 L/min—sufficient for rapid removal of supernatants, media, wash buffers, and spent reagents without compromising sterility or operator safety. Designed specifically for biosafety-conscious environments, the system eliminates lubricant-based contamination risks, operates at low acoustic noise (<52 dB(A)), and maintains mechanical robustness across extended duty cycles.
Key Features
- Oil-Free Piston Pump Architecture: Eliminates need for lubricant replenishment, reduces particulate and hydrocarbon outgassing, and ensures compliance with ISO 5 cleanroom-compatible aspiration practices.
- Dual Overflow Protection System: Combines a mechanically actuated float valve inside the 1200 mL PES waste bottle with an in-line 0.2 µm hydrophobic PTFE disc filter positioned between the bottle and pump head—preventing liquid ingress into the vacuum motor under overfill or sudden pressure fluctuation conditions.
- Ergonomic Handheld Controller with Lock Function: Features a palm-contoured POM housing and tactile push-button with integrated lock mechanism, enabling sustained aspiration without finger fatigue during prolonged procedures such as confluence monitoring or multi-well plate processing.
- Single-Hand Tip Ejection Mechanism: Patented octo-claw tip connector allows reliable, one-handed detachment of standard 200 µL or 1000 µL pipette tips—reducing repetitive motion strain and minimizing cross-contamination risk during high-throughput operations.
- Modular & Autoclavable Component Design: All fluid-path components—including PES waste bottle, POM single/dual claw tip adapters, stainless steel optional connectors, and 150 mm long single-tip adapters—are rated for repeated autoclaving at 121°C for 20 minutes per ISO 17665-1, supporting GLP/GMP-aligned decontamination protocols.
- Space-Efficient Integrated Platform: Compact footprint (35 × 24.3 × 29 cm) with recessed bottle cradle and perimeter containment ridge prevents accidental tipping during active use—a critical design consideration in crowded biosafety cabinets and laminar flow hoods.
Sample Compatibility & Compliance
The Lafil300 Plus accommodates a wide range of aqueous and mildly corrosive biological liquids, including DMEM/F12, PBS, trypsin-EDTA solutions, antibiotic-containing media, and clarified lysates. It is not intended for organic solvents, strong acids/bases, or viscous suspensions (>50 cP). The system meets EN 61000-6-3 (EMC emission) and EN 61000-6-2 (immunity) requirements and carries full CE marking per Directive 2014/30/EU (EMC) and 2014/35/EU (LVD). While not FDA 510(k)-cleared, its design principles align with USP guidance on auxiliary equipment used in aseptic processing and supports audit readiness for ISO 13485 and ISO 9001 quality management systems. All wetted materials comply with USP Class VI biocompatibility testing standards.
Software & Data Management
The Lafil300 Plus operates as a standalone hardware system with no embedded microprocessor or digital interface. Consequently, it does not generate electronic records, require firmware updates, or support data export—making it inherently compliant with environments where electronic audit trails are either unnecessary or deliberately avoided to minimize validation burden. Its mechanical simplicity ensures uninterrupted functionality in resource-limited labs and eliminates cybersecurity vulnerabilities associated with connected instrumentation. For laboratories requiring traceability, manual log entries (e.g., date, operator ID, procedure type, bottle change timestamp) can be maintained in paper-based or LIMS-integrated formats per internal SOPs.
Applications
- Aspiration of spent culture media from adherent and suspension cell cultures in T-flasks, roller bottles, and multi-well plates.
- Removal of supernatants following centrifugation or sedimentation steps in primary cell isolation protocols.
- Vacuum-assisted buffer exchange in dialysis and desalting workflows.
- Drainage of wash solutions during immunostaining, ELISA, and Western blotting procedures.
- Controlled liquid removal in stem cell differentiation assays where shear stress minimization is critical.
- Supporting biosafety level 2 (BSL-2) containment operations when paired with appropriate HEPA-filtered exhaust manifolds.
FAQ
Is the Lafil300 Plus suitable for aspiration of ethanol or isopropanol?
No. The system is designed exclusively for aqueous biological fluids. Organic solvents may degrade PES and POM components and compromise the integrity of the PTFE filter.
Can the vacuum pressure be adjusted or monitored in real time?
No. The pump delivers fixed performance parameters; there is no built-in vacuum gauge or pressure regulation circuitry.
What is the expected service life of the oil-free piston pump under normal lab usage?
Rated for >10,000 hours of cumulative operation with periodic inspection of intake filters and O-rings per manufacturer-recommended maintenance intervals.
Are replacement PTFE filters and PES bottles available as consumables?
Yes. ROCKER supplies genuine spare parts under catalog numbers 197000-11 (PES bottle) and 197305-22 (complete Lafil300 Plus kit), ensuring consistent filtration efficiency and dimensional compatibility.
Does the system meet requirements for Good Manufacturing Practice (GMP) environments?
While not certified for Grade A cleanrooms, its autoclavable components, absence of lubricants, and CE conformity enable use in GMP-supporting laboratory areas where equipment qualification (IQ/OQ) is performed per internal validation protocols.
