Analytik Jena Smart Extraction Nucleic Acid Purification System

Overview

The Analytik Jena Smart Extraction Nucleic Acid Purification System represents a paradigm shift in molecular sample preparation—replacing conventional solid-phase binding chemistries with a proprietary, patent-protected liquid-phase extraction principle. Unlike traditional methods relying on phenol-chloroform partitioning, silica-membrane columns, centrifugal filtration, vacuum manifolds, or magnetic bead capture, Smart Extraction operates via selective ion-exchange-mediated phase separation in aqueous solution. This chemistry-driven approach enables direct lysis and nucleic acid partitioning within a single reaction vessel, eliminating mechanical handling steps such as centrifugation, bead pelleting, or column washing. The system is engineered for high reproducibility, minimal carryover risk, and compatibility with variable input volumes—from 200 µL to 10 mL of whole blood, saliva, tissue homogenates, or environmental samples—making it especially suited for biobanking, clinical diagnostics, and large-scale epidemiological studies where throughput and consistency are critical.

Key Features

• Patent-protected liquid-phase nucleic acid isolation technology—no solid-phase matrices (e.g., silica membranes, magnetic beads) required
• Single-tube workflow: lysis, binding, and elution occur in one vessel; no transfer, centrifugation, or vacuum steps
• Minimal hands-on time: only pipette tip aspiration and dispensing (up-and-down pipetting) needed for mixing and phase separation
• High yield and integrity: consistently delivers double-stranded DNA ≥ 50 kb and full-length RNA (RIN ≥ 9.0) from challenging samples including FFPE, bacterial cultures, and low-biomass microbiome specimens
• Scalable format support: validated for manual use and seamless integration into Analytik Jena’s automated platforms—including InnuPure C16, InnuPure C16 touch, InnuPure C96, GeneTheatre, CyBi-SELMA, and CyBi-FeliX
• Reduced contamination risk: closed-tube processing minimizes aerosol generation and cross-sample carryover

Sample Compatibility & Compliance

The Smart Extraction platform demonstrates broad compatibility across human, animal, plant, microbial, and viral nucleic acid sources. It has been validated per ISO 20387:2018 (Biobanking—General requirements for biobank operations) and supports GLP/GMP-aligned workflows through traceable reagent lot tracking and instrument audit logs. All Smart Extraction kits comply with EU IVDR 2017/746 Annex II performance evaluation requirements for in vitro diagnostic nucleic acid extraction reagents. When deployed on CyBi-FeliX or InnuPure C96 systems, the workflow meets FDA 21 CFR Part 11 requirements for electronic records and signatures, including user authentication, activity logging, and immutable data archiving.

Software & Data Management

Smart Extraction workflows are fully programmable via Analytik Jena’s CyBi-Control Suite and InnuPure Control Software. These platforms provide protocol versioning, parameter locking, and real-time run monitoring with deviation alerts. Raw process metadata—including pipetting volume, mixing cycles, incubation times, and temperature profiles—is automatically embedded into output files using vendor-neutral JSON-LD schema. Exported data integrates natively with LIMS environments via HL7 v2.5 and ASTM E1384-compliant interfaces. Audit trails comply with ALCOA+ principles (Attributable, Legible, Contemporaneous, Original, Accurate, Complete, Consistent, Enduring, Available), supporting regulatory submissions to EMA, FDA, and PMDA.

Applications

• High-yield genomic DNA isolation from >5 mL whole blood for long-read sequencing (PacBio SMRT, Oxford Nanopore)
• Intact total RNA purification from fibrous tissues (e.g., heart, skeletal muscle) without RNase inhibition loss
• Simultaneous co-isolation of DNA and RNA from single samples for multi-omics profiling
• Routine extraction in clinical labs requiring CLIA/CAP-compliant turnaround times (<12 min/sample on C96)
• Environmental metagenomics: direct extraction from soil, water, and air filter matrices with minimal inhibitor carryover
• Automated extraction for SARS-CoV-2, influenza A/B, and RSV detection in high-volume diagnostic laboratories

FAQ

Does Smart Extraction require specialized instrumentation to operate?
No—core chemistry functions manually with standard pipettes and ambient-temperature incubators. However, full automation requires Analytik Jena’s InnuPure or CyBi series instruments.
Can Smart Extraction be used for both DNA and RNA isolation?
Yes—dedicated kit formulations exist for genomic DNA, total RNA, miRNA, and simultaneous DNA/RNA co-extraction, each optimized for respective binding kinetics and elution pH.
Is the technology compatible with downstream qPCR, NGS library prep, and digital PCR?
All eluates meet Qubit™, NanoDrop™, and Bioanalyzer™ QC thresholds for NGS library construction (Illumina TruSeq, ThruPLEX, NEBNext) and quantitative applications (TaqMan®, SYBR® Green).
How does Smart Extraction handle samples with high levels of inhibitors (e.g., humic acid, heparin, polysaccharides)?
The ion-exchange matrix selectively excludes common PCR inhibitors while retaining nucleic acids—validation data show ≥92% removal efficiency for heparin and ≥85% for humic substances without additional cleanup steps.
Are Smart Extraction reagents supplied as single-use kits or bulk formats?
Available in both configurations: pre-aliquoted single-use kits (C16/C96-ready) and bulk reagent packs for custom automation scripting and cost-optimized high-throughput deployment.