Sanotac HT-50 High-Pressure Preparative Liquid Chromatography System for Flavonoid Isolation and Purification

Overview

The Sanotac HT-50 is a modular, high-pressure preparative liquid chromatography (HPPLC) system engineered for reproducible, scalable isolation and purification of natural product compounds—particularly flavonoids—from complex botanical matrices such as traditional Chinese medicinal herbs. It operates on the principle of reversed-phase or normal-phase liquid chromatography under controlled high-pressure conditions (up to 30 MPa), enabling efficient separation based on differential polarity, molecular size, and hydrogen-bonding affinity. The system integrates a dual-piston, PEEK- or 316L stainless-steel pump head, a full-spectrum UV-Vis detector (190–800 nm), and an automated fraction collector—all synchronized via Sanochrom™ control software compliant with regulatory data integrity standards. Designed specifically for laboratories engaged in phytochemical research, natural product chemistry, and preclinical compound supply, the HT-50 delivers robust performance across gradient elution, peak-triggered collection, and multi-wavelength monitoring—critical for resolving structurally similar flavonoid aglycones and glycosides.

Key Features

• High-precision binary or quaternary gradient pumping system with flow range 0.01–50.00 mL/min and accuracy ±1% (RSD ≤0.5%)
• 30 MPa maximum system pressure capability, supporting C18, C8, phenyl-hexyl, and amino-silica preparative columns (10–50 mm ID)
• Dual-lamp UV-Vis detector (deuterium + tungsten) with simultaneous dual-wavelength acquisition and ±1 nm wavelength accuracy
• Automated fraction collector accommodating 2 × 60 tubes (Φ15 mm × 150 mm); optional configurations support 24-, 48-, or 96-well plates
• Flexible injection modes: solid loading (via sample loop + dissolution protocol) and liquid injection (10 mL standard loop, with optional 5–50 mL loops)
• Modular architecture compatible with columns and accessories from Biotage®, Cytiva AKTA™, ISCO® Companion, Buchi® Pure, and Bio-Rad® NGC systems
• Sanochrom™ PC-based software with intuitive graphical interface, USB/RS-232 connectivity, and audit-trail-enabled operation

Sample Compatibility & Compliance

The HT-50 is routinely deployed for the purification of flavonoid subclasses—including flavones (e.g., apigenin), flavonols (e.g., quercetin, kaempferol), isoflavones (e.g., genistein, daidzein), chalcones, anthocyanidins, and biflavonoids—from crude extracts of Chrysanthemum morifolium, Scutellaria baicalensis, Glycyrrhiza uralensis, Epimedium brevicornum, and Rhodiola rosea. Its low baseline noise (<±0.75×10⁻⁵ AU at 254 nm) and high wavelength repeatability (0.2 nm) ensure reliable quantitation and peak integration for trace-level glycoside resolution. The system meets core requirements of GLP and GMP-aligned workflows: software enforces electronic signatures, user access levels, and immutable audit trails per FDA 21 CFR Part 11 and CFDA GxP guidelines. All wetted parts (pump heads, flow cells, tubing) are chemically resistant to common solvents (MeOH, ACN, water, 0.1% formic acid, 0.1% TFA), and optional PEEK components enhance compatibility with acidic or basic mobile phases.

Software & Data Management

Sanochrom™ software provides end-to-end method development, execution, and reporting functionality. Users define gradient profiles (linear, step, or custom), set real-time collection triggers (time-based, threshold-based, or peak-area gated), and assign tube positions dynamically during runs. Raw chromatograms, spectral overlays, and fraction metadata are stored in vendor-neutral .csv and .chm formats. The software supports export to third-party analytical tools (e.g., Chromeleon, Empower, OpenLab) and includes built-in calibration wizards for flow rate, pressure, and absorbance linearity verification. All operational events—including parameter changes, login/logout, and fraction collection logs—are timestamped and cryptographically signed to satisfy regulatory inspection readiness. Validation documentation packages (IQ/OQ/PQ protocols) are available upon request for laboratory accreditation (ISO/IEC 17025, CNAS).

Applications

The HT-50 serves as a primary platform for isolating reference standards and milligram-to-gram quantities of bioactive flavonoids used in pharmacological screening, structure–activity relationship (SAR) studies, and formulation development. Documented applications include: purification of apigenin-7-glucoside (≥98.5% purity) from chrysanthemum; isolation of scutellarin (≥98.0%) from Erigeron breviscapus; preparative-scale recovery of icariin (≥98.0%) and epimedins A–C from epimedium; and separation of structural isomers such as naringin vs. neohesperidin in citrus peel extracts. Its adaptability to both low-flow semi-preparative (1–10 mL/min) and high-flow preparative (20–50 mL/min) regimes allows seamless method translation from analytical HPLC to production-scale purification—reducing development time and solvent consumption.

FAQ

Is the HT-50 compatible with columns from other manufacturers?
Yes—the system uses industry-standard 1/4″ and 1/8″ fittings and supports column dimensions up to 50 mm ID. It is validated for use with Biotage SNAP KP, Cytiva Tricorn, ISCO RediSep Rf, and Buchi C-Prep columns.
Does the software support 21 CFR Part 11 compliance?
Yes—Sanochrom™ implements role-based access control, electronic signatures, and permanent audit trails for all critical actions, satisfying FDA and NMPA regulatory expectations.
What is the minimum detectable absorbance change for flavonoid peaks?
At 254 nm with 1-second time constant, baseline noise is ±0.75×10⁻⁵ AU, enabling confident detection of sub-microgram injections in optimized UV-absorbing fractions.
Can the system perform pH-sensitive separations?
Yes—optional PEEK pump heads, flow cells, and tubing minimize metal-catalyzed degradation, making the HT-50 suitable for acidic (e.g., TFA-containing) or basic (e.g., ammonium acetate) mobile phases commonly used for flavonoid glycoside resolution.
Is method transfer from analytical to preparative scale supported?
Yes—Sanochrom™ includes scaling calculators that maintain k′ and α values across column geometries, ensuring retention time predictability and reducing method re-optimization effort.