Yiheng LRH-250F Biochemical Incubator

Overview

The Yiheng LRH-250F Biochemical Incubator is an engineered-for-precision environmental chamber designed for stable, reproducible temperature-controlled cultivation of microorganisms, cell cultures, and biological samples in research, quality control, and regulatory-compliant laboratory environments. Utilizing a hermetically sealed compressor refrigeration system with R134a refrigerant — selected for its zero ozone depletion potential (ODP) and low global warming potential (GWP) — the unit delivers consistent thermal performance across its full 0–60 °C operating range. Its forced-air convection system ensures uniform heat distribution while minimizing thermal stratification, critical for applications requiring high spatial repeatability such as BOD incubation, fungal propagation, and seed germination assays. The incubator’s structural integrity, mirror-finish stainless steel interior (304 grade), and seamless quarter-radius corners support ISO 14644-1 Class 8 cleanroom-compatible cleaning protocols and reduce microbial retention points.

Key Features

• Mirror-polished 304 stainless steel inner chamber with fully rounded corners — facilitates rapid decontamination and complies with CDC/NIH biosafety cabinet surface hygiene standards;
• Microprocessor-based PID temperature controller with 0.1 °C resolution and real-time digital display — supports programmable ramp-hold profiles and automatic calibration offset adjustment;
• Intelligent fan speed modulation — dynamically adjusts airflow velocity based on setpoint deviation to suppress sample desiccation during extended incubation cycles;
• Dual-stage overtemperature protection: primary electronic cut-off + independent mechanical thermostat — certified per IEC 61010-1 Annex BB for lab equipment safety;
• RS485 serial interface (standard) and optional thermal printer port — enables integration into centralized lab monitoring systems (e.g., LabVantage, Thermo Fisher SampleManager) and supports 21 CFR Part 11–compliant audit trails when paired with validated software;
• Energy-optimized design: high-efficiency rotary compressor, vacuum-insulated double-wall construction (polyurethane foam, 45 mm thickness), and low-thermal-conductivity door gasket — reduces power consumption by up to 22% versus legacy models in continuous 37 °C operation.

Sample Compatibility & Compliance

The LRH-250F accommodates standard microbiological vessels including Petri dishes (up to 150 mm), test tubes (16 × 150 mm), Erlenmeyer flasks (up to 2 L), and multi-well plates (6–96-well formats). Adjustable stainless steel shelving (3 standard trays, load capacity 15 kg per shelf) permits flexible configuration for heterogeneous sample loads. The unit meets fundamental design criteria aligned with ISO 13408-1 (aseptic processing), ASTM E1752-22 (incubator performance verification), and USP (pharmaceutical stability testing environments). While humidity control is not integrated, its sealed chamber design maintains ambient RH stability within ±5% over 24 h at 25 °C — sufficient for non-hygroscopic culture media and enzymatic assays.

Software & Data Management

Through its RS485 interface, the LRH-250F supports Modbus RTU protocol for bidirectional communication with SCADA or LIMS platforms. Firmware v2.3+ includes time-stamped event logging (power cycles, alarm triggers, parameter changes) stored onboard for ≥30 days. When connected to validated third-party data acquisition software (e.g., WinWedge Pro, LabVIEW DAQmx), it generates CSV-formatted temperature logs compliant with ALCOA+ data integrity principles. Optional thermal printer output provides hard-copy traceability for GLP audits — printouts include date/time stamp, setpoint, actual chamber temperature, and operator ID field (when integrated with network authentication).

Applications

• Biochemical oxygen demand (BOD₅) analysis per APHA Standard Methods 5210B;
• Microbial enumeration (total viable count, coliforms, Pseudomonas spp.) under ISO 4833-1 and FDA BAM Chapter 3;
• Fungal culture maintenance (Aspergillus, Candida, Fusarium) at 25–30 °C with low-volatility preservation;
• Plant tissue culture initiation and callus proliferation (e.g., Arabidopsis thaliana, Oryza sativa);
• Enzyme activity profiling and protein stability screening in pharmaceutical development;
• Environmental simulation studies for aquatic toxicology (OECD 201, 210) and soil respiration assays.

FAQ

Does the LRH-250F support humidity control?
No — this model is a temperature-only incubator. For combined temp/RH applications, consider the Yiheng PH-250 series.
Is the unit suitable for GMP-regulated environments?
Yes — when operated with documented IQ/OQ protocols, calibrated against NIST-traceable references, and linked to a 21 CFR Part 11–validated data system.
What is the maximum shelf loading weight?
15 kg per shelf; total payload capacity is 45 kg across three shelves.
Can the incubator operate continuously at 4 °C?
Yes — the compressor-based cooling system is rated for uninterrupted operation from 0–60 °C, including sub-ambient conditions.
Are calibration certificates included with shipment?
Factory calibration certificate (temperature uniformity & fluctuation at 25 °C and 37 °C) is provided; UKAS/NIST-traceable certification is available upon request.