Ortoalresa Digicen 22 R Refrigerated Benchtop Centrifuge

Overview

The Ortoalresa Digicen 22 R is a high-performance refrigerated benchtop centrifuge engineered for precision separation of biological macromolecules—including proteins, nucleic acids, organelles, and subcellular particles—in research, clinical, and quality control laboratories. Designed and manufactured in Spain with over three decades of centrifugation expertise, the Digicen 22 R operates on the principle of sedimentation under controlled gravitational force (RCF), enabling reproducible pelleting, layering, and clarification across diverse sample types. Its integrated refrigeration system maintains the chamber at ≤4 °C throughout operation—critical for thermolabile biomolecules—and functions independently of rotor type or speed setting, ensuring thermal stability even during extended runs. The instrument supports both fixed-angle and swing-out rotor configurations, offering flexibility for differential and density-gradient centrifugation protocols without requiring platform reconfiguration.

Key Features

• Microprocessor-controlled brushless induction motor delivering maintenance-free operation and long-term rotational stability.
• REI (Rotor Easy Installation) system: tool-free, one-hand rotor mounting and release with mechanical auto-recognition and secure locking.
• Intuitive LCD interface displaying real-time RPM, RCF, elapsed/remaining time, chamber temperature, and acceleration/deceleration profiles (PCBS).
• Programmable parameters in 10 rpm / 10 × g increments; timer range from 1 s to 99 min with ±5 s accuracy and hold-at-end functionality.
• 100 user-defined protocols stored in non-volatile memory; up to 175 customizable acceleration and braking ramps to minimize post-centrifugation resuspension.
• Acoustic and visual alerts for lid status, imbalance, temperature deviation, rotor mismatch, and emergency stop conditions.
• Comprehensive safety architecture: dual electric lid lock, automatic imbalance shutdown, overspeed cut-off, drop-proof lid mechanism, and reinforced stainless-steel chamber with protective safety ring.
• Energy-saving mode with automatic power-down after 8 hours of inactivity; rotors and adapters compatible with steam sterilization (121 °C, 20 min, 1 bar).

Sample Compatibility & Compliance

The Digicen 22 R accommodates a broad spectrum of primary and secondary containers—from microtubes (0.2–2.0 mL) and PCR strips to conical tubes (15 mL, up to 32 positions) and large-volume bottles (100 mL, 4-position swing-out rotor). Its fixed-angle rotors support high-speed pelleting of small-volume samples, while swing-out configurations enable gentle separation of fragile gradients or viscous suspensions. All rotors are CE-marked and certified to EN 61010-2-101 (safety requirements for laboratory centrifuges) and EN 61010-2-020 (requirements for centrifuges used in medical laboratories). The device complies with EU directives 2014/35/EU (Low Voltage Directive), 2014/30/EU (EMC Directive), 2011/65/EU (RoHS), and 98/79/EC (IVDR), and adheres to environmental regulations (EC) No 1005/2009 (ozone-depleting substances) and (EU) No 517/2014 (F-gas regulation).

Software & Data Management

Ortoalresa SmartConnect—a free cross-platform application—enables remote monitoring, configuration, and control via Wi-Fi-connected PCs, tablets, or smartphones. Users can initiate, pause, or abort runs; download protocol logs; and verify operational history without physical access to the instrument. While the Digicen 22 R does not natively support 21 CFR Part 11-compliant electronic signatures or audit trails, its event logging (including start/stop timestamps, rotor ID, set parameters, and fault codes) meets GLP-aligned documentation standards for traceability in non-regulated academic and industrial labs. Exportable CSV logs facilitate integration into LIMS environments for batch record compilation.

Applications

• Isolation of plasmid DNA, genomic DNA, and RNA from bacterial, mammalian, and plant lysates.
• Purification of exosomes, microvesicles, and extracellular nanoparticles via differential ultracentrifugation (with compatible high-speed rotors).
• Subcellular fractionation of mitochondria, nuclei, lysosomes, and peroxisomes using sucrose or iodixanol density gradients.
• Clarification of cell culture supernatants, fermentation broths, and bioprocess harvests prior to downstream chromatography.
• Preparative separation of antibodies, enzymes, and recombinant proteins during purification workflows.
• Routine clinical applications including serum/plasma separation, PBMC isolation, and urinalysis sediment concentration.

FAQ

What is the maximum RCF achievable with the Digicen 22 R?
The instrument delivers a maximum relative centrifugal force of 26,480 × g at its top speed of 16,500 rpm.
Can all rotors be autoclaved?
Yes—both fixed-angle and swing-out rotors, along with all standard adapters, are rated for steam sterilization at 121 °C for 20 minutes.
Does the Digicen 22 R support gradient centrifugation?
Yes, when equipped with swing-out rotors and compatible tube holders, it enables rate-zonal and isopycnic separations using sucrose, glycerol, or cesium chloride gradients.
How does the REI system improve workflow efficiency?
The REI system eliminates torque wrenches and alignment tools—rotors click into place with tactile feedback and auto-identify via embedded RFID, reducing setup time and operator error.
Is remote operation possible without local network infrastructure?
SmartConnect requires connection to a local Wi-Fi network; standalone Ethernet or direct USB control is not supported.