Agilent 1200 Prep High-Performance Liquid Chromatograph
| Brand | Agilent Technologies |
|---|---|
| Origin | Canada |
| Manufacturer Type | Authorized Distributor |
| Origin Category | Imported |
| Model | 1200, G1315B |
| Price Range | USD 13,500 – 40,500 (FOB) |
| Product Category | Chromatography |
| Operational Age | 3–4 years |
| Warranty | 6 months |
Overview
The Agilent 1200 Prep High-Performance Liquid Chromatograph is a modular, field-proven preparative-scale HPLC system engineered for robust fractionation, purification, and method development in pharmaceutical, academic, and contract research laboratory environments. Built upon Agilent’s proven 1200 Series architecture, this refurbished system employs low-dispersion dual-piston reciprocating pump technology (G1361A), UV/Vis diode-array detection (G1315B DAD), precision autosampling (G2258A or G2260A), and programmable fraction collection (G1364B) to deliver reproducible separation performance across milligram-to-gram sample loads. The system operates on standard reversed-phase, normal-phase, and ion-exchange chromatographic principles, with flow rate stability ≤0.1% RSD and pressure pulsation <1% at maximum rated pressure — characteristics validated per ASTM E260 and ISO 17025-compliant verification protocols during refurbishment.
Key Features
- Two Agilent 1200 Series preparative pumps (G1361A): Dual-gradient capability up to 100 mL/min, 400 bar max pressure, pulse-free flow delivery optimized for column loading and solvent compatibility with C18, silica, and amino columns.
- Agilent 1200 Series Diode Array Detector (G1315B): 190–950 nm spectral range, 1 nm resolution, 80 Hz acquisition rate, and real-time peak purity analysis — enabling wavelength-specific fraction triggering and post-run spectral deconvolution.
- Preparative autosampler options: Either the dual-loop G2258A (2 × 100 µL loops, 0.1–5000 µL injection volume) or the high-capacity G2260A (up to 5 mL loop, needle-in-loop design for viscous or particulate-laden samples).
- Intelligent fraction collector (G1364B): Supports timed, peak-based, or threshold-triggered collection into 12-mm or 16-mm test tubes, microtiter plates, or custom vial racks; includes UV-synchronized trigger logic and position calibration traceability.
- Refurbishment protocol: Performed by SpectraLab Scientific to ISO 9001-certified standards — includes full hydraulic integrity testing, detector wavelength accuracy verification (NIST-traceable holmium oxide filter), pump seal replacement, and firmware update to latest stable revision (Rev. B.04.0x).
Sample Compatibility & Compliance
This Agilent 1200 Prep system accommodates a broad range of sample matrices, including synthetic organic intermediates, natural product extracts, peptide libraries, and small-molecule APIs. Column compatibility spans 10–50 mm ID preparative columns (e.g., ZORBAX PrepHT, Poroshell 120) and analytical guard columns. All hardware components meet CE marking requirements and conform to IEC 61010-1 for laboratory equipment safety. Data acquisition and system control comply with ALCOA+ data integrity principles; audit trails, electronic signatures, and user access controls can be implemented via optional OpenLab CDS software — supporting FDA 21 CFR Part 11 readiness when deployed in regulated GLP/GMP workflows.
Software & Data Management
The system operates natively with Agilent ChemStation Rev. A.10.02 or higher, providing method editor, sequence scheduler, real-time chromatogram display, and integrated fraction log export (CSV/XML). For enhanced compliance, OpenLab CDS 2.x is fully compatible and enables secure instrument control, electronic lab notebook (ELN) integration, and automated report generation compliant with USP Analytical Instrument Qualification guidelines. All refurbished units ship with original Agilent documentation sets, including IQ/OQ templates, maintenance logs, and calibration certificates traceable to NIST standards.
Applications
- Purification of reaction mixtures prior to NMR or MS structural confirmation
- Isolation of chiral enantiomers using polysaccharide-based preparative columns
- Desalting and buffer exchange of oligonucleotides and synthetic peptides
- Stability-indicating method development for forced degradation studies (per ICH Q1–Q5)
- Scale-up support from analytical to semi-preparative conditions using retention time and selectivity transfer algorithms
FAQ
What validation documentation is included with the refurbished Agilent 1200 Prep system?
A complete refurbishment dossier is provided: pump flow accuracy report (±1% tolerance), DAD wavelength verification certificate (holmium oxide filter), pressure sensor calibration record, and functional test chromatograms for baseline noise, drift, and peak symmetry.
Can this system be integrated into an existing OpenLab CDS network?
Yes — all modules (pumps, DAD, autosampler, fraction collector) retain native LAN connectivity and are recognized automatically within OpenLab CDS 2.3 or later; no hardware modification is required.
Is extended warranty or service contract available beyond the standard 6-month coverage?
SpectraLab Scientific offers optional 12- or 24-month extended service agreements covering parts, labor, and on-site preventive maintenance visits — fully customizable to regional regulatory requirements.
Are spare parts and consumables readily available?
All G13xx-series modules use field-serviceable, cross-compatible consumables (e.g., G1312-60012 pump seals, G1315-60005 DAD lamp); inventory is maintained globally through Agilent’s authorized channel partners.
