Agilent 1220 Infinity LC System (Refurbished)

Overview

The Agilent 1220 Infinity LC System (Refurbished, Model G4294B) is a high-integrity, performance-validated liquid chromatography platform engineered for robust HPLC and method-compatible UHPLC operation. Built on Agilent’s proven Infinity architecture, this system employs low-dispersion flow paths, dual-piston parallel pumping technology, and real-time pressure monitoring to deliver precise gradient formation, excellent retention time reproducibility (<0.05% RSD), and stable baseline performance across extended runs. Designed for laboratories transitioning from legacy HPLC platforms or scaling routine QC/QA capacity without full UHPLC capital investment, the 1220 Infinity supports column internal diameters from 2.1 mm to 4.6 mm and maximum operating pressures up to 600 bar—enabling method transfer from older Agilent 1100/1200 systems and compatibility with modern sub-2-µm particle columns under optimized conditions. Its modular design, compact footprint (38 × 50 × 47 cm), and low power consumption make it especially suitable for space-constrained environments—including mobile analytical labs and field-deployable QC units.

Key Features

• Quaternary gradient pump (G1312B) with active solvent level sensing and pulse-free flow delivery; flow range: 0.001–5.0 mL/min, accuracy ±0.2%, precision <0.1% RSD
• High-performance autosampler (G1329B) with 100-position tray capacity, injection volume range 0.1–100 µL, carryover <0.005%
• Thermostatted column compartment (G1316A) maintaining temperature stability ±0.15 °C from 5–80 °C
• Diode array detector (G1315D) with 1024-pixel linear CCD array, wavelength range 190–640 nm, scan rate up to 80 Hz, and photometric accuracy ±0.002 AU at 254 nm
• Fully refurbished by certified Agilent service partners: all fluidic components replaced or reconditioned, optics cleaned and calibrated, electronics validated per Agilent Performance Qualification (PQ) protocols
• Pre-installed OpenLab CDS software (v2.x or later) with audit trail, electronic signatures, and 21 CFR Part 11 compliance modules enabled

Sample Compatibility & Compliance

The 1220 Infinity G4294B accommodates a broad range of sample matrices—including pharmaceutical actives, environmental extracts, food additives, and biological fluids—when paired with reversed-phase, HILIC, or ion-exchange columns. It meets critical regulatory requirements for routine analysis in GLP and GMP environments: hardware and firmware are traceable to Agilent’s original manufacturing lot records; refurbishment includes full IQ/OQ documentation per ISO/IEC 17025 guidelines; and system validation reports include linearity (r² >0.999 over 3–4 orders of magnitude), LOD/LOQ determination, and system suitability testing per USP and EP 2.2.46. All refurbished units undergo 72-hour continuous operational stress testing prior to shipment.

Software & Data Management

Controlled via Agilent OpenLab CDS (ChemStation Edition or OpenLab ECM), the system supports method development, sequence management, peak integration, and report generation with configurable templates. Audit trail functionality logs all user actions—including method edits, data reprocessing, and calibration events—with immutable timestamps and operator ID linkage. Data files (.ch, .cdf) are stored in secure, folder-based repositories with optional integration into LIMS via ASTM E1384 or HL7 interfaces. Backups comply with FDA 21 CFR Part 11 Annex 11 requirements, including role-based access control, digital signature enforcement, and encrypted archive export.

Applications

• Pharmaceutical QC: Assay, dissolution testing, and impurity profiling per ICH Q2(R2) guidelines
• Food & Beverage: Pesticide residue screening (AOAC 2007.01), mycotoxin quantitation (EN 14123), and vitamin analysis
• Environmental: PAHs, PCBs, and phenolic compounds in water/soil extracts (EPA Methods 8330B, 8082A)
• Academic & Contract Research: Method development support, stability-indicating assays, and forced degradation studies
• Mobile Lab Deployment: Integrated power conditioning, shock-absorbing chassis mounting, and simplified pneumatic interface for vehicle-based operation

FAQ

Is this unit covered by Agilent’s original warranty?
No—this is a professionally refurbished system with a 6-month limited hardware warranty covering pump seals, detector lamps, autosampler syringes, and column oven heaters.
Can I use this system for USP/EP-compliant release testing?
Yes—provided your laboratory completes site-specific OQ/PQ using documented protocols aligned with ISO/IEC 17025 and maintains full traceability of calibration standards and reference materials.
Does the system include a computer and monitor?
Yes—each unit ships with a dedicated Windows 10 Pro workstation preloaded with OpenLab CDS and validated drivers; no additional hardware purchase is required for immediate operation.
What documentation accompanies the instrument?
You receive a full refurbishment certificate, PQ report, electrical safety test record, chromatographic performance verification data, and a complete set of electronic manuals (PDF) and firmware update history.
Is remote technical support available after installation?
Yes—Spectralab provides 24-month post-delivery email and screen-sharing support for method troubleshooting, software configuration, and preventive maintenance scheduling.