Agilent 1260 Infinity II HPLC System with 1290 Infinity II Column Compartment

Overview

The Agilent 1260 Infinity II HPLC System—integrated with the 1290 Infinity II Column Compartment—is a high-performance, second-hand liquid chromatography platform engineered for precision, robustness, and method continuity in regulated and research laboratory environments. Built upon Agilent’s proven quaternary solvent delivery architecture, this system employs dual-piston positive displacement pumping with active solvent compressibility compensation, enabling stable gradient formation and exceptional retention time reproducibility (< 0.05 min RSD over 24 h). The 1260 Infinity II pump supports operation up to 600 bar, facilitating use of both conventional 3–5 µm packed columns and advanced sub-2 µm particle columns—including core-shell (superficially porous) media—without hardware modification. Coupled with the 1290 Infinity II column compartment (G1330B), which delivers ±0.15 °C temperature stability across a 5–80 °C range and features low thermal mass design for rapid equilibration, the system achieves enhanced peak shape symmetry, reduced backpressure drift, and improved inter-run reproducibility—critical for quantitative bioanalysis, QC release testing, and method transfer workflows.

Key Features

• Quaternary low-dead-volume solvent manager with integrated degasser (G1322A), supporting precise binary, ternary, or quaternary gradient elution
• High-pressure, low-pulsation binary pump (G1312B) with real-time pressure monitoring and automatic leak detection
• Autosampler (G1329B) with 100-position tray capacity, needle wash station, and injection volume range from 0.1–100 µL with ≤ 0.25% RSD precision
• Thermostatted column compartment (G1330B) offering active air-cooling/heating, dual-zone temperature control, and column tracking via RFID-enabled rack identification
• Column oven (G1316A) with independent temperature control (5–80 °C) and low thermal inertia design for rapid method optimization
• UV-Vis detector (G4208B) featuring 1024-element diode array, 1–4 nm spectral resolution, and data acquisition rates up to 100 Hz for high-speed separations
• Full backward compatibility with legacy ChemStation methods and seamless migration path to OpenLab CDS 2.x for 21 CFR Part 11 compliance

Sample Compatibility & Compliance

This refurbished Agilent 1260/1290 HPLC system accommodates a broad spectrum of sample matrices—including small-molecule pharmaceuticals, peptides, natural products, environmental extracts, and food additives—when paired with appropriate stationary phases (C18, HILIC, phenyl-hexyl, etc.) and mobile phase chemistries. All modules have been functionally validated per Agilent’s original factory specifications and verified against ISO/IEC 17025-aligned internal calibration protocols. The system meets essential performance criteria outlined in USP , EP 2.2.46, and ASTM D7593 for HPLC system suitability. When deployed with validated OpenLab CDS software, it supports electronic signatures, audit trails, user access controls, and instrument configuration logging required under GLP, GMP, and FDA-regulated environments.

Software & Data Management

The system operates natively with Agilent OpenLab CDS (ChemStation Edition or CDS 2.x), providing full instrument control, sequence management, peak integration, calibration curve generation, and report templating. Raw data files (.D files) are stored in vendor-neutral formats compliant with ASTM E2524 and are fully exportable to third-party platforms (e.g., Empower, Chromeleon, ACD/Labs) via ASCII or mzML conversion. Audit trail functionality records all critical events—including method edits, injection execution, parameter changes, and user logins—with immutable timestamps. Data integrity is preserved through encrypted storage, role-based permissions, and optional networked backup integration with enterprise NAS or LIMS systems.

Applications

• Pharmaceutical QC/QA: Assay, related substances, dissolution testing, and stability-indicating assays per ICH Q2(R2)
• Biopharmaceutical analysis: Peptide mapping, residual host-cell protein quantitation, and monoclonal antibody purity profiling
• Environmental monitoring: Pesticide residue screening, PAH quantification, and PFAS analysis in water and soil extracts
• Food & beverage safety: Mycotoxin detection, vitamin profiling, and adulterant screening using multi-residue methods
• Academic & contract research: Method development, column evaluation studies, and kinetic modeling of retention behavior

FAQ

Is this system eligible for Agilent factory service contracts?

Yes—refurbished 1260/1290 systems retain full eligibility for Agilent Care extended service plans, subject to hardware verification and firmware version compliance.
Can legacy ChemStation methods be directly imported into OpenLab CDS?

Yes—OpenLab CDS includes a built-in ChemStation method converter that preserves gradient profiles, integration events, and calibration tables without manual reconfiguration.
What validation documentation is provided with the system?

Each unit ships with a comprehensive Certificate of Performance Verification, including pressure accuracy, flow precision, injection repeatability, UV wavelength accuracy, and column oven temperature uniformity test reports.
Does the 6-month warranty cover software updates and remote diagnostics?

The warranty includes hardware repair, firmware updates, and remote technical support via Agilent’s authorized service network; software license renewal requires separate subscription.
Are replacement parts and consumables readily available?

All major components—including pump seals, autosampler rotors, lamp assemblies, and column compartment fans—are stocked by Agilent distributors globally and supported by standard lead times under 5 business days.