Agilent 6210 TOF LC/MS System (Refurbished)

Overview

The Agilent 6210 TOF LC/MS System is a refurbished orthogonal acceleration time-of-flight (oa-TOF) mass spectrometer engineered for high-resolution, accurate-mass analysis in liquid chromatography–mass spectrometry workflows. Unlike quadrupole or ion trap systems, the 6210 employs pulsed orthogonal extraction to decouple ion injection from detection timing—enabling sub-microsecond duty cycles, mass accuracy better than 5 ppm (external calibration), and resolving power exceeding 10,000 FWHM across a broad m/z range (up to 20,000 Da). Its oa-TOF architecture delivers linear dynamic range >10⁴ and isotopic fidelity suitable for elemental composition determination, structural elucidation, and untargeted profiling in pharmaceutical, environmental, and life science laboratories. This unit was fully reconditioned and performance-verified by SpectraLab Scientific against Agilent’s original factory specifications—including mass calibration stability, sensitivity (e.g., 1 pg/µL reserpine S/N ≥ 50:1 at m/z 609.28 in full-scan mode), and retention time reproducibility (<0.1% RSD over 10 injections).

Key Features

• Orthogonal acceleration TOF design enabling simultaneous high mass accuracy and high acquisition speed (up to 5 spectra/sec)
• Integrated Agilent 1200 Series LC platform with binary pump (G1312A), autosampler (G1313A), column oven (G1316A), and degasser (G1322A)
• Modular ion source compatibility: electrospray (ESI), atmospheric pressure chemical ionization (APCI), atmospheric pressure photoionization (APPI), and multimode ionization (MMI)
• Flow-rate agnostic architecture supporting conventional (0.2–2 mL/min), microbore (1–100 µL/min), and nanoflow (50–500 nL/min) chromatographic configurations
• MassHunter Data Acquisition and Qualitative Analysis software with real-time centroiding, formula prediction, and MS/MS-triggered acquisition (via external trigger signal)
• Hardware-level compliance with 21 CFR Part 11 requirements: electronic signatures, role-based user permissions, and immutable audit trails enabled via MassHunter’s Security Configuration module

Sample Compatibility & Compliance

The 6210 TOF LC/MS system accommodates diverse sample matrices—including small molecules, peptides, oligonucleotides, and polar metabolites—without requiring derivatization. Its wide linear mass range (m/z 50–20,000) and low chemical noise floor support robust quantitation in complex biological extracts (e.g., plasma, urine, tissue homogenates) when coupled with appropriate LC gradients and source optimization. The system meets analytical requirements outlined in ISO/IEC 17025:2017 for testing laboratories and aligns with method validation principles described in ICH Q2(R2). All refurbished units undergo full functional verification per Agilent’s Service Manual Rev. D, including vacuum integrity checks (≤5×10⁻⁷ Torr base pressure), detector gain calibration, and ion optic alignment validation.

Software & Data Management

Controlled exclusively through Agilent MassHunter Workstation (vB.07.00 or higher), the system provides traceable, compliant data handling. MassHunter integrates native support for ASTM E1947-compliant metadata embedding, automated report generation (PDF/HTML), and secure database archiving (via optional MassHunter Quantitative or BioConfirm modules). Audit trails record all parameter changes, calibration events, and user logins with timestamps and operator IDs. Raw data files (.d format) are stored in vendor-neutral, non-proprietary structures compatible with third-party processing tools (e.g., Skyline, MZmine, OpenMS) via standard mzML export.

Applications

• High-confidence identification of unknown compounds in environmental monitoring (e.g., PFAS, pesticides, pharmaceutical residues)
• Peptide mapping and post-translational modification analysis in biopharmaceutical characterization
• Metabolite profiling in pharmacokinetic and toxicokinetic studies
• Impurity identification and structural confirmation per ICH Q3B(R2) guidelines
• Method development for regulated bioanalysis where accurate mass and reproducible retention behavior are critical

FAQ

Is this system eligible for Agilent’s extended warranty or service contracts?

Yes—refurbished 6210 systems retain full eligibility for Agilent CrossLab service agreements, including Preventive Maintenance (PM) plans and response-time-guaranteed support.
Does the included MassHunter license support quantitative analysis?

The system ships with MassHunter Qualitative Analysis B.07.x; Quantitative Analysis licenses are available as optional add-ons and fully interoperable.
Can the instrument be validated for GxP environments?

Yes—the hardware and software configuration satisfies baseline requirements for IQ/OQ/PQ execution; SpectraLab provides URS templates and commissioning documentation aligned with ASTM E2500 and EU Annex 11.
What consumables and spare parts are recommended for immediate deployment?

We recommend stocking ESI needles (G1969-60001), capillary columns (ZORBAX RRHD 300SB-C18, 2.1 × 50 mm), and calibration standards (G1969-85000 ESI-L Tuning Mix) prior to installation.