Agilent Cary 8454 UV-Visible Spectrophotometer (Refurbished)

Overview

The Agilent Cary 8454 UV-Visible Spectrophotometer is a benchtop, single-beam photodiode array (PDA) spectrophotometer engineered for precision, stability, and regulatory readiness in quality control, pharmaceutical analysis, academic research, and industrial R&D environments. Leveraging over three decades of Agilent’s PDA optical architecture expertise, the Cary 8454 employs a fixed grating and solid-state detector design—eliminating moving optical components—to deliver high photometric accuracy, exceptional baseline stability, and sub-second full-spectrum acquisition (190–1100 nm). Its optical path is thermally stabilized and sealed against ambient fluctuations, ensuring reproducible absorbance measurements across extended operational cycles. Designed specifically for multi-user laboratory workflows, the system integrates seamlessly into regulated environments through built-in self-diagnostic routines, electronic audit trails, and firmware-level compliance controls.

Key Features

• Photodiode Array Detection: Simultaneous acquisition across the full UV-Vis range (190–1100 nm) with 1 nm nominal resolution—no wavelength scanning required.
• Zero-Moving-Parts Optics: Fixed monochromator architecture minimizes mechanical drift and enhances long-term photometric reproducibility (RSD <0.002 A at 0.5 A).
• Premounted, Pre-Calibrated Lamps: Factory-aligned deuterium (UV) and tungsten-halogen (Vis/NIR) sources enable tool-free replacement with no realignment needed.
• Regulatory-Ready Firmware: Embedded GLP features include instrument self-tests, user-accessible calibration logs, time-stamped method versioning, and password-protected parameter locking.
• 21 CFR Part 11 Compliant Software Stack: UV-Vis ChemStation software supports electronic signatures, audit trail export, and role-based access control when deployed with OpenLab ECM.
• Modular Hardware Expansion: Compatible with temperature-controlled cuvette holders (±0.1 °C), 8-position autosamplers, flow cells, and multi-cell changers—all controlled natively within ChemStation.

Sample Compatibility & Compliance

The Cary 8454 accommodates standard 10 mm square quartz, glass, or plastic cuvettes (pathlengths 1–100 mm), as well as microvolume adapters and flow-through cells for liquid-phase analysis. It meets critical pharmacopeial requirements including United States Pharmacopeia (USP) (Spectrophotometric Absorbance), European Pharmacopoeia (EP) 2.2.25 (Ultraviolet and Visible Absorption Spectrophotometry), and Japanese Pharmacopoeia (JP) 6.07. All refurbished units undergo full functional verification per Agilent’s original factory test protocols—including wavelength accuracy (±0.2 nm), photometric linearity (0–3.0 A), and stray light (<0.05%T at 220 nm)—with documented traceability to NIST-traceable standards. Each instrument ships with a Certificate of Reconditioning and a 6-month limited warranty covering optics, electronics, and mechanical subsystems.

Software & Data Management

Controlled exclusively via Agilent UV-Vis ChemStation software (v7.02 or later), the Cary 8454 supports method-driven workflows—from simple concentration quantitation using Beer-Lambert law to kinetic assays, spectral deconvolution, and multi-component analysis. Data files are stored in secure, encrypted .D files compliant with ALCOA+ principles (Attributable, Legible, Contemporaneous, Original, Accurate, Complete, Consistent, Enduring, Available). Audit trails record all user actions—including method edits, calibration events, and result exports—with immutable timestamps and operator IDs. When integrated with Agilent OpenLab ECM, the system satisfies full 21 CFR Part 11 requirements, including electronic signature validation, workflow-based approval routing, and centralized backup with retention policy enforcement.

Applications

• Pharmaceutical QC release testing: assay, dissolution, impurity profiling, and excipient compatibility studies.
• Academic teaching labs: hands-on instruction in spectroscopic theory, quantitative analysis, and method validation.
• Chemical manufacturing: raw material identification, reaction monitoring, and colorimetric endpoint detection.
• Environmental labs: nitrate/nitrite, phosphate, and heavy metal speciation via colorimetric assays.
• Food & beverage: carotenoid quantification, antioxidant capacity (e.g., DPPH), and pigment stability assessment.

FAQ

Is this unit fully refurbished and tested to original Agilent specifications?
Yes. Each Cary 8454 undergoes comprehensive optical, electronic, and mechanical recalibration at our ISO 17025-accredited service facility, with full performance validation against Agilent’s OEM test plan.
Does the 6-month warranty cover lamp replacement?
Yes—the warranty includes labor and parts for both deuterium and tungsten lamps, provided replacement is performed by authorized service personnel.
Can I upgrade to OpenLab ECM post-purchase?
Absolutely. The instrument ships with ChemStation v7.02 preinstalled and licensed for OpenLab ECM integration; license activation and server deployment support are available upon request.
Are NIST-traceable calibration reports included?
Yes—each unit ships with a signed Certificate of Reconditioning and a full calibration report referencing NIST SRM 2034 (peak wavelength) and SRM 930e (photometric accuracy).
What documentation accompanies the system?
You receive the original Agilent user manual (digital), firmware update history, calibration certificates, Certificate of Reconditioning, and a detailed refurbishment checklist signed by our senior service engineer.