AMANI Series Micro-pH Electrodes
| Origin | USA |
|---|---|
| Manufacturer Type | Authorized Distributor |
| Origin Category | Imported |
| Model | AMANI Series |
| Pricing | Available Upon Request |
Overview
The AMANI Series micro-pH electrodes are precision-engineered solid-state electrochemical sensors designed for high-fidelity pH measurement in ultra-small, sensitive, or physically constrained biological and pharmaceutical samples. Unlike conventional glass pH electrodes, the AMANI platform employs a proprietary non-glass, all-plastic architecture with an integrated leak-free, non-porous reference junction—eliminating liquid junction potential drift, protein fouling, and clogging risks common in traditional Ag/AgCl-based systems. These electrodes operate on the Nernstian principle, delivering stable, reproducible potentials across pH 0–14 with slope performance consistently ≥95% of theoretical (59.16 mV/pH at 25 °C). Their miniaturized design enables direct intracellular, microfluidic, tissue slice, and in vivo microenvironmental monitoring—applications where sample volume is limited to sub-microliter quantities and mechanical fragility must be avoided.
Key Features
- All-plastic construction: Chemically inert, shatterproof, and compatible with sterilization protocols including autoclaving (up to 121 °C, 15 psi, 20 min) and ethylene oxide exposure.
- Leak-free non-porous reference junction: Eliminates electrolyte leakage, prevents contamination of samples, and ensures long-term stability in protein-rich, surfactant-laden, or viscous media—including serum, cell lysates, and hydrogel matrices.
- Ultrafast response kinetics: Achieves <3-second stabilization time for step changes up to 9 pH units—critical for real-time kinetic assays and dynamic physiological monitoring.
- Exceptional chemical resistance: Validated for continuous use in aggressive media such as hydrofluoric acid (HF), concentrated organic solvents (e.g., DMSO, acetonitrile), and low-ionic-strength buffers—where conventional electrodes fail rapidly.
- Flexible probe variants: Available with polyimide-coated stainless-steel shafts for use on microscope stages, within micromanipulator setups, or inside narrow-bore catheters and capillary tubing.
- Zero-maintenance operation: No internal filling solution required; fully functional after dry storage or rehydration—ideal for intermittent use in GLP-compliant labs and field-deployable diagnostic platforms.
Sample Compatibility & Compliance
The AMANI Series supports measurement in volumes as low as 250 nL—enabling analysis of single-cell cytosol, microdroplets, microtiter well edge zones, and microextractions from tissue biopsies. Its inert surface minimizes adsorption of biomolecules, ensuring integrity of pharmacokinetic and metabolic profiling data. The electrodes comply with ISO 3696 (Grade 3 water purity testing), ASTM E70 (standard test method for pH of aqueous solutions), and support FDA 21 CFR Part 11–compliant data acquisition when paired with validated pH meters featuring audit-trail functionality. They are routinely employed in USP and compliant compounding environments due to absence of mercury or heavy-metal components.
Software & Data Management
AMANI electrodes interface seamlessly with any standard BNC-equipped pH meter, potentiostat, or data acquisition system (e.g., National Instruments DAQ, LabVIEW, MATLAB, or commercial benchtop analyzers from Mettler Toledo, Thermo Fisher, or Hanna Instruments). When used with GLP/GMP-certified instrumentation, full traceability—including electrode ID, calibration history, temperature compensation logs, and raw mV readings—is preserved. Optional OEM integration kits provide analog/digital output modules for embedding into custom IVD devices or automated clinical analyzers requiring CE-IVDR or UL 61010-1 certified signal conditioning.
Applications
- Intracellular and extracellular pH mapping in live neurons, cardiomyocytes, and organoids under confocal or patch-clamp configurations.
- Real-time acidification monitoring during microbial fermentation, cell culture bioprocesses, and enzyme kinetics assays.
- pH profiling in microfluidic organ-on-chip platforms and droplet-based digital PCR workflows.
- Stability testing of biologics and mRNA-LNP formulations under accelerated stress conditions (e.g., low-pH hold steps).
- Neuropharmacological studies involving synaptic vesicle acidification, lysosomal pH perturbation, and ionophore-mediated proton flux.
- Point-of-care diagnostics development—integrated into handheld electrochemical readers for rapid wound fluid or tear film analysis.
FAQ
Can AMANI electrodes be calibrated using standard pH buffer sets?
Yes—calibration follows ISO 8655 and ASTM E70 protocols using NIST-traceable pH 4.01, 7.00, and 10.01 buffers. Two-point calibration is sufficient for most applications; three-point is recommended for extended-range work.
Do these electrodes require storage in KCl solution?
No—dry or hydrated storage is acceptable. Prolonged immersion in high-ionic-strength solutions is discouraged to preserve junction integrity.
Is temperature compensation supported?
Yes—when connected to a meter with automatic temperature compensation (ATC), the AMANI Series delivers accurate pH values across 0–80 °C, with built-in thermal coefficient matching per Nernst equation.
Can they be used in conductive but non-aqueous media?
Limited validation exists for low-water-content organic solvents (e.g., <10% v/v water in ethanol); performance should be verified empirically for each matrix.
What is the typical service life under routine laboratory use?
With proper handling and cleaning (e.g., mild detergent rinse followed by deionized water), operational lifetime exceeds 18 months—significantly longer than glass electrodes in comparable bioanalytical workflows.
