DS MB100-6A Microplate Incubator Shaker with Temperature Control

Overview

The DS MB100-6A Microplate Incubator Shaker is an integrated orbital shaker and precision temperature-controlled incubation platform engineered for reproducible, high-throughput microplate-based assays in life science, pharmaceutical, and clinical research laboratories. It combines thin-film resistive heating with PID-based intelligent temperature regulation to deliver rapid thermal equilibration and stable thermal environments across the entire plate surface. Unlike ambient-only shakers, the MB100-6A maintains precise setpoint control (0–100 °C) while simultaneously executing uniform orbital agitation—enabling kinetic studies, enzymatic reactions, cell culture expansion, antigen-antibody binding, and microbial growth assays under tightly defined thermal and mechanical conditions. Its design addresses critical workflow constraints: minimizing edge effects via a fully环绕ed (circumferential) heated lid, supporting multi-plate parallel processing without cross-contamination risk, and ensuring long-duration stability required for overnight incubations.

Key Features

• Orbital shaking mechanism with adjustable speed (200–1600 rpm) and fixed 3 mm amplitude—optimized for consistent mixing in 96-, 384-, and deep-well microplates without splashing or well-to-well variation.
• Dual-mode heated lid system: programmable automatic or manual thermal control, reducing evaporation and thermal gradient formation at plate peripheries—critical for low-volume assays and extended incubations.
• Independent timer function for each of up to six microplates, enabling staggered start/stop sequences and heterogeneous protocol execution within a single run.
• DC brushless motor drive ensures silent operation (<50 dB(A)), electromagnetic compatibility with sensitive detection instruments (e.g., plate readers, luminometers), and maintenance-free longevity.
• Real-time LED display showing setpoints and actual values for temperature, speed, and remaining time—alongside status indicators for heating, shaking, lid engagement, and safety interlocks.
• Dual-layer safety architecture: hardware-based overtemperature cutoff (independent of controller) plus software-enforced thermal limits; automatic fan-assisted cooling upon shutdown or temperature overshoot.
• Onboard temperature calibration utility (±0.1 °C resolution) allows user verification and adjustment against traceable NIST-certified reference probes—supporting internal quality assurance procedures.
• Auto-restart after power interruption preserves experimental integrity; preheat mode enables thermal stabilization prior to sample loading, reducing ramp-time variability between runs.

Sample Compatibility & Compliance

The MB100-6A accommodates industry-standard SBS-format microplates—including flat-bottom, U/V-bottom 96- and 384-well ELISA plates, adherent and suspension cell culture plates, and 2 mL deep-well blocks (e.g., PCR-compatible polypropylene). Its flame-retardant ABS construction complies with UL 94 HB flammability requirements. While not certified for ISO 13485 or FDA 21 CFR Part 11 out-of-the-box, the device supports GLP/GMP-aligned workflows through audit-trail-capable logging (when paired with optional RS232/USB data export), configurable password-protected parameter locking, and documented calibration protocols. It meets IEC 61010-1 safety standards for laboratory electrical equipment and operates within electromagnetic emission limits per CISPR 11 Group 1, Class B.

Software & Data Management

The MB100-6A operates autonomously via its embedded firmware, eliminating dependency on external PCs. All parameters—including multi-point temperature ramps (up to 5 segments), cyclic programs (up to 99 loops), and independent plate timers—are configured directly via front-panel keys. Optional PC connectivity (RS232 or USB) enables remote monitoring, batch parameter upload/download, and time-stamped CSV log export (temperature, speed, elapsed time) for integration into LIMS or electronic lab notebooks. Firmware updates are delivered via USB flash drive, ensuring traceability and version control in regulated environments.

Applications

• Enzyme kinetics and immunoassay development (e.g., ELISA, CLIA), where simultaneous temperature control and gentle agitation enhance binding efficiency and reduce assay time.
• Mammalian and insect cell culture—particularly for suspension-adapted lines requiring continuous motion during expansion or transfection recovery phases.
• Microbial growth profiling under varied thermal-shake conditions (e.g., MIC/MBC determination, biofilm dispersion studies).
• Protein solubilization, antibody labeling, and nucleic acid hybridization protocols demanding strict thermal homogeneity and minimal shear stress.
• Quality control testing of reagent stability, lyophilized product reconstitution, and diagnostic kit validation per ISO 15189 or CLIA guidelines.

FAQ

Does the MB100-6A support programming multiple temperature steps within a single run?
Yes—it supports up to five sequential temperature setpoints with dwell times, enabling complex thermal profiles such as heat-denaturation followed by controlled annealing.
Can the heated lid be disabled for ambient-temperature shaking only?
Yes—the lid heating can be turned off independently while retaining full speed and timing functionality.
Is the device compatible with 384-well plates?
Yes—its platform design and orbital dynamics ensure uniform energy transfer across all well geometries, including high-density formats.
What is the maximum allowable load per plate position?
Each plate position supports up to 200 g total mass (including plate, cover, and liquid volume); exceeding this may affect thermal uniformity and mechanical stability.
How is temperature uniformity verified across the plate surface?
Uniformity specifications (≤±0.5 °C at 37 °C) are validated using nine-point mapping per ASTM E2251–22, with calibrated thermocouples placed at center and corner wells of a loaded 96-well plate.