Hanuo THZ-82A Digital Speed-Controlled Water Bath Shaker

Overview

The Hanuo THZ-82A Digital Speed-Controlled Water Bath Shaker is an engineered platform for precise temperature-regulated orbital agitation of liquid samples in life science laboratories. It operates on the principle of water bath thermal conduction—where a thermostatically controlled water medium ensures uniform, stable heat transfer across the entire shaker platform—combined with a brushless DC motor-driven orbital motion system. This dual-function design enables simultaneous control of temperature (ambient to 100 °C) and oscillation frequency (0–300 rpm), making it suitable for applications requiring both thermal stability and mechanical homogenization, such as microbial culture expansion, enzyme kinetics assays, solubility testing, and hybridization protocols. Unlike air-jacketed shakers, the water bath configuration minimizes thermal gradients and reduces evaporation during extended incubation, supporting reproducible experimental conditions over time.

Key Features

• Microprocessor-based digital controller with independent LED displays for real-time monitoring of set and actual temperature and rotational speed.
• Orbital shaking motion (circular trajectory) with fixed 20 mm amplitude, optimized for suspension culture uniformity and minimal shear stress on sensitive biological samples.
• Stainless steel (AISI 304) inner chamber and platform—resistant to corrosion from aqueous buffers, saline solutions, and mild organic solvents commonly used in molecular biology workflows.
• Spring-loaded universal bottle holder accommodates standard Erlenmeyer flasks (50–1000 mL), test tubes, and multi-well plates without auxiliary clamps or adapters.
• Timer function with 0–120 minute range; auto-shutdown upon completion ensures energy efficiency and sample integrity during unattended operation.
• No-splash oscillation design—achieved via balanced motor mounting and rigid platform support—prevents cross-contamination and maintains sterility in shared lab environments.
• Heating power: 1800 W, enabling rapid thermal equilibration and stable maintenance within ±0.5 °C across the full operating range.

Sample Compatibility & Compliance

The THZ-82A supports routine use with aqueous-based biological media including LB broth, M9 minimal medium, PBS, Tris-HCl buffers, and protein storage solutions. Its stainless steel construction complies with ISO 8573-1 for compressed air purity class requirements when integrated into cleanroom-adjacent workflows. While not certified to GLP or GMP standards out-of-the-box, the instrument’s digital logging capability (via optional RS-232/USB interface) allows integration into validated laboratory information management systems (LIMS) for audit-trail generation per FDA 21 CFR Part 11 guidelines. The device meets IEC 61010-1 safety requirements for laboratory electrical equipment and conforms to electromagnetic compatibility (EMC) standards EN 61326-1.

Software & Data Management

The THZ-82A features embedded firmware with non-volatile memory to retain user-defined parameters (temperature setpoint, speed, timer duration) after power cycling. Optional serial communication (RS-232 or USB-to-serial adapter) enables remote parameter adjustment and real-time data streaming to PC-based acquisition software. When connected to compliant LIMS platforms, the shaker can contribute timestamped operational logs—including start/stop events, temperature deviation alerts, and speed consistency checks—for traceability in quality-controlled environments such as QC labs in pharmaceutical manufacturing or academic core facilities adhering to ISO/IEC 17025.

Applications

• Bacterial and yeast culture propagation under controlled thermal and agitation conditions.
• Enzyme activity profiling across defined temperature gradients (e.g., 30–45 °C) with concurrent mixing to prevent sedimentation.
• DNA/RNA hybridization and probe labeling protocols requiring gentle, continuous agitation at elevated temperatures (e.g., 65 °C).
• Solubility and dissolution testing of small-molecule compounds in pharmacokinetic studies.
• Cell lysis optimization using detergent-based or enzymatic methods where temperature-sensitive reagents demand tight thermal regulation.
• Environmental microbiology: enrichment cultures of thermophilic or mesophilic isolates from soil/water matrices.

FAQ

What is the maximum load capacity per flask on the THZ-82A platform?
The spring-mounted platform supports up to 5 kg total load, distributed across standard 1000 mL Erlenmeyer flasks (typically ≤2.5 kg per flask when filled to 75% volume).
Can the THZ-82A be operated without water in the bath?
No. This is a water bath shaker; operation without water will damage the heating element and invalidate thermal calibration.
Is external calibration of temperature or speed required?
Factory-calibrated at point-of-manufacture; users may perform periodic verification using NIST-traceable RTD probes and tachometers per internal SOPs.
Does the unit include over-temperature protection?
Yes—a redundant bimetallic thermal cutoff switch activates at 105 °C to prevent dry-run or runaway heating scenarios.
How often should the water bath be refilled or replaced?
Deionized water should be changed weekly in high-use labs; biocide additives are not recommended unless validated for compatibility with stainless steel and sample integrity.