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HUXI Semi-Disposable Sterilizable Pipette, 0.1–2.5 µL

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Brand HUXI
Origin Shanghai, China
Manufacturer Type OEM/ODM Producer
Country of Origin China
Model 0.1–2.5 µL
Instrument Type Single-Channel Manual Pipette
Automation Level Manual
Channel Count 1
Volume Range 0.1–2.5 µL
Maximum Capacity 2.5 µL
Accuracy ±3.0% at 2.5 µL
Increment 0.05 µL
Sterilization Method Autoclavable (121 °C, 20 min)
Tip Ejector Removable and Autoclavable
Ergonomic Design Palm-Hook Handle
Display High-Contrast Volume Window
Accessories Included Pipette Hook, Tip Ejector Removal Tool

Overview

The HUXI Semi-Disposable Sterilizable Pipette (0.1–2.5 µL) is a precision-engineered single-channel manual pipette designed for ultra-low-volume liquid handling in molecular biology, genomics, high-throughput screening, and diagnostic assay development. Operating on the air displacement principle, it delivers consistent volumetric delivery across its full range with calibrated piston mechanics and low-resistance tip attachment. Its design prioritizes traceability, repeatability, and compliance with international laboratory standards—particularly where minimal sample consumption, contamination control, and routine sterilization are critical. The pipette’s lightweight aluminum alloy body and balanced center-of-gravity reduce operator fatigue during extended use, while its semi-disposable architecture—featuring a removable, autoclavable tip ejector and chemically resistant internal seals—enables rigorous decontamination without compromising mechanical integrity.

Key Features

  • Ergonomic palm-hook handle design allows secure, fatigue-free grip and one-handed operation—ideal for repetitive pipetting tasks in PCR setup or NGS library preparation.
  • High-contrast volume display window with dual-scale graduation ensures rapid, error-resistant setting verification at 0.05 µL increments.
  • Removable tip ejector mechanism withstands repeated autoclaving (121 °C, 20 min, 100+ cycles), supporting GLP/GMP-aligned maintenance protocols and eliminating cross-contamination risks.
  • Chemically inert internal components resist degradation from common solvents (e.g., ethanol, isopropanol, dilute acids/bases), extending service life and preserving calibration stability.
  • Calibration-friendly design includes external adjustment screw and factory-certified traceable calibration certificate (ISO/IEC 17025 accredited lab).
  • Includes dedicated accessories: stainless-steel tip ejector removal tool and wall-mounted pipette hook for organized benchtop storage.

Sample Compatibility & Compliance

This pipette is validated for use with standard conical polypropylene tips (0.1–10 µL universal fit) and compatible with viscous, volatile, or temperature-sensitive aqueous solutions—including DNA/RNA suspensions, enzyme master mixes, and fluorescent dyes. It meets ISO 8655-2:2022 requirements for accuracy and precision of piston-operated volumetric apparatus. While not certified to FDA 21 CFR Part 11, its calibration documentation supports audit-ready records for ISO 9001, ISO 13485, and CLIA-regulated environments. All materials comply with RoHS 2011/65/EU and REACH SVHC guidelines.

Software & Data Management

As a manually operated instrument, this pipette does not incorporate embedded software or digital connectivity. However, its calibration data—including as-delivered accuracy values at three volume points (0.25 µL, 1.25 µL, and 2.5 µL), uncertainty budgets, and traceable reference standards—is provided in PDF format compliant with ISO/IEC 17025 reporting conventions. Laboratories may integrate calibration logs into LIMS or ELN platforms using standardized templates (e.g., ASTM E2500 Annex A3). Annual recalibration is recommended per ISO 8655-5:2022 guidance to maintain ≤±3.0% accuracy at maximum volume.

Applications

  • Quantitative reverse transcription and qPCR reaction setup requiring sub-microliter precision.
  • CRISPR-Cas9 guide RNA complex assembly and titration in functional genomics workflows.
  • Microfluidic device priming and nanoliter-scale reagent dispensing in organ-on-chip systems.
  • Calibration of secondary standards in metrology labs performing volumetric verification of automated liquid handlers.
  • Quality control testing of low-concentration therapeutic proteins or oligonucleotide therapeutics under ICH Q5C stability protocols.

FAQ

Is this pipette suitable for handling corrosive or high-salt buffers?
Yes—the piston seal and barrel interior are constructed from PTFE-coated stainless steel and medical-grade elastomers, demonstrating compatibility with 1 M NaCl, 10 mM DTT, and 70% ethanol. Avoid prolonged exposure to concentrated strong oxidizers (e.g., >5% sodium hypochlorite).

Can the entire pipette be autoclaved?
No—only the removable tip ejector and lower sleeve assembly are autoclavable. The main body must be surface-cleaned with 70% ethanol; full autoclaving would compromise spring tension and optical encoder alignment.

What is the recommended recalibration interval?
Per ISO 8655-5:2022, recalibrate every 6–12 months depending on usage frequency and criticality of application. High-use environments (>500 actuations/day) require quarterly verification.

Does HUXI provide calibration services outside China?
Yes—HUXI partners with ISO/IEC 17025-accredited third-party labs in the EU, US, and Singapore for on-site or return-to-lab calibration with NIST-traceable standards.

Are replacement tip ejectors available separately?
Yes—HUXI part number HX-PE-025 is stocked globally and supplied with individual CE/UKCA declarations of conformity.

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