KD Scientific Legato 130 Microinfusion Syringe Pump for Stereotaxic Surgery

Overview

The KD Scientific Legato 130 Microinfusion Syringe Pump is an engineered solution for high-precision, low-volume fluid delivery in neuroscience and preclinical research applications—particularly stereotaxic brain injections in rodent and small-animal models. Designed around a closed-loop microstepping stepper motor architecture and precision-machined lead-screw actuation, the Legato 130 delivers exceptional volumetric reproducibility across its full operational range—from picoliter-per-minute infusion rates using sub-microliter syringes to milliliter-scale delivery with larger barrels. Its core measurement principle relies on deterministic plunger displacement control rather than pressure-based feedback, ensuring consistent flow kinetics independent of backpressure fluctuations commonly encountered during intracranial cannula insertion or viscous neuropharmaceutical formulations. The pump’s mechanical design prioritizes minimal vibrational coupling—critical when integrated with micromanipulators or stereotaxic frames—while maintaining compliance with international safety and electromagnetic compatibility standards (CE, UL, CSA, CB Scheme) and environmental directives (RoHS, WEEE).

Key Features

• 4.3-inch WQVGA color touchscreen interface with intuitive, icon-driven navigation—optimized for glove-compatible operation in biosafety cabinets or laminar flow hoods
• Modular controller–pumphead architecture enabling remote mounting of the pump head up to 2 meters from the control unit via shielded cabling, facilitating seamless integration with stereotaxic apparatuses and motorized micromanipulators
• Preloaded syringe database covering >120 commercially available glass and plastic syringes (0.5–1000 µL), with full support for user-defined syringe geometry (barrel ID, plunger travel, dead volume)
• Two independent programmable protocols, each supporting up to 50 discrete steps—including variable flow rate, dwell time, direction (infuse/withdraw), and repeat loops—enabling complex multi-phase injection paradigms (e.g., priming → slow infusion → washout)
• Hardware-level password protection (admin/user tiers) and non-volatile memory retention ensure protocol integrity and audit-ready configuration persistence between power cycles
• Dual communication interfaces: isolated RS485 bus (supporting daisy-chained networks of up to 99 pumps) and USB Type-B for direct PC connectivity; optional TTL-triggered I/O for synchronization with electrophysiology rigs or behavioral monitoring systems
• Robust all-metal chassis and vibration-damped linear drive train reduce acoustic noise and mechanical resonance—essential for long-duration infusions in awake or lightly sedated preparations

Sample Compatibility & Compliance

The Legato 130 supports standard Luer-lock and small-bore polypropylene/glass syringes ranging from 0.5 µL capillaries to 1000 µL reservoirs, accommodating both aqueous and moderately viscous solutions (e.g., viral vectors, fluorescent tracers, siRNA complexes, and nanoparticle suspensions). It complies with ISO 8536-4 (syringes for medical use), ASTM F2337 (performance testing of infusion pumps), and FDA 21 CFR Part 11 requirements when used with validated Adagio software configurations. All firmware and hardware revisions undergo GLP-aligned verification protocols—including traceable calibration against NIST-traceable flow standards—and maintain full documentation for regulatory submissions under ICH-GCP and AAALAC-accredited animal study frameworks.

Software & Data Management

The optional Adagio PC software provides advanced remote orchestration capabilities, including real-time flow monitoring, synchronized multi-pump coordination, automated protocol validation, and export of timestamped CSV logs compliant with ALCOA+ data integrity principles. Adagio supports audit trail generation (user actions, parameter changes, execution events), electronic signatures, and role-based access control—meeting requirements for GMP-aligned neuropharmacology studies and IND-enabling toxicology assessments. Raw motion data (step count, direction, elapsed time) can be streamed via RS485 for third-party acquisition platforms such as Spike2, MATLAB, or National Instruments LabVIEW.

Applications

• Stereotaxic delivery of AAV, lentivirus, or CRISPR-Cas9 constructs into discrete murine brain nuclei (e.g., hippocampus, striatum, ventral tegmental area)
• Chronic intracerebroventricular (ICV) or intraparenchymal infusion for pharmacokinetic/pharmacodynamic modeling
• Co-injection of tracer dyes with therapeutics for post-hoc histological verification of injection site fidelity
• Microdialysis probe priming and zero-flow calibration in awake, freely moving subjects
• High-throughput screening of neuroactive compounds in zebrafish or C. elegans larval models using capillary-based microinjection rigs

FAQ

What syringe sizes are supported?
The Legato 130 accommodates syringes from 0.5 µL (capillary) to 1000 µL (standard glass/plastic), with automatic scaling of flow resolution based on barrel inner diameter.
Can it operate in withdraw mode for aspiration or sampling?
Yes—the pump supports bidirectional operation (infuse and withdraw) with identical accuracy and programmability in both directions.
Is the pump compatible with third-party stereotaxic frames?
Yes—its compact footprint (22.6 × 17.78 × 9.32 cm), low-profile mounting options, and remote pump-head capability ensure mechanical compatibility with major platforms including Stoelting, David Kopf, and Narishige systems.
Does it meet regulatory requirements for GLP or GMP studies?
When deployed with Adagio software under documented configuration management and periodic performance verification, the system satisfies key elements of FDA 21 CFR Part 11, EU Annex 11, and ISO/IEC 17025 for instrument qualification in regulated environments.
How is flow accuracy verified and maintained?
Accuracy (±0.5%) is validated at time of manufacture using gravimetric methods traceable to NIST SRM 2822; users may perform routine verification using calibrated microbalances and timed collection assays per SOP-SP-003 (available in KD Scientific Technical Documentation Library).