PerkinElmer LS-45 Fluorescence Spectrophotometer (Refurbished)

Overview

The PerkinElmer LS-45 Fluorescence Spectrophotometer is a benchtop, pulsed-xenon-based luminescence measurement system engineered for quantitative and qualitative analysis of fluorescent, phosphorescent, chemiluminescent, and bioluminescent samples. Designed as an evolutionary upgrade to the LS-50B platform, the LS-45 implements dual-monochromator optics with independently adjustable excitation and emission slits—enabling high spectral resolution, minimal stray light, and precise control over bandwidth selection. Its pulsed xenon source eliminates continuous thermal load, minimizes photodegradation of labile analytes, and enables time-resolved detection without auxiliary hardware. The instrument operates on a fixed optical path geometry optimized for signal stability and reproducibility across extended measurement sessions. All critical optical alignments, detector calibration, and lamp energy profiling are verified during refurbishment to meet original PerkinElmer performance benchmarks.

Key Features

• Dual-grating monochromators with motorized slit drives for independent excitation (2.5–15 nm) and emission (2.5–20 nm) bandwidth control in 0.1 nm increments
• Pulsed xenon lamp (≥10⁹ pulses lifetime) with integrated dark-current compensation between pulses—essential for low-intensity phosphorescence decay kinetics
• Five-position automated emission filter wheel (290, 350, 390, 430, 515 nm) plus software-selectable 1% neutral density attenuation for dynamic range extension
• Scan speed programmability from 10 to 1500 nm/min in 1 nm steps; time-based data acquisition mode supports kinetic and lifetime studies
• Signal-to-noise ratio ≥750:1 RMS (water Raman peak at 350 nm excitation) and ≥2500:1 RMS (baseline), validated per ASTM E1318-15 methodology
• Refurbished unit includes full mechanical inspection, optical alignment verification, photomultiplier tube (PMT) gain recalibration, and firmware update to latest stable release

Sample Compatibility & Compliance

The LS-45 accommodates standard 10 mm quartz cuvettes, solid-sample holders (including powder and film stages), and microvolume adapters (down to 50 µL). Its large sample compartment allows integration of temperature-controlled cells, polarization accessories, and fiber-optic probes—all compatible with PerkinElmer’s legacy FL WinLab™ driver architecture. Refurbished units undergo rigorous functional testing per ISO/IEC 17025-aligned protocols, including wavelength accuracy verification using holmium oxide and didymium filters, photometric linearity assessment via neutral density series, and long-term intensity stability monitoring (>8 hours). Documentation includes Certificate of Refurbishment, traceable calibration records, and conformance statement to USP , ICH Q2(R2), and FDA 21 CFR Part 11 audit-trail requirements when operated with compliant software configurations.

Software & Data Management

The LS-45 is fully supported by PerkinElmer’s FL WinLab™ v4.x software suite, which provides comprehensive method development, multi-dimensional data visualization (2D contour plots, 3D surface rendering), and automated spectral deconvolution tools. Data files are stored in proprietary .spc format with embedded metadata (instrument ID, operator, timestamp, parameter set), exportable to ASCII, CSV, or JCAMP-DX for third-party analysis. Software installation includes licensed activation, digital signature validation, and optional GLP-compliant user access controls (role-based permissions, electronic signatures, and audit trail logging). All refurbished systems ship with verified software media, license key, and documented installation logs.

Applications

This instrument serves core analytical functions across pharmaceutical QC (e.g., assay of riboflavin, quinine sulfate, and tetracycline derivatives), environmental monitoring (PAHs, humic substances), materials science (quantum dot quantum yield, OLED emissive layer characterization), and life sciences (protein folding via intrinsic tryptophan fluorescence, NAD(P)H redox state quantification, calcium indicator kinetics). Its phosphorescence capability—enabled by pulse-delay-gated acquisition—supports oxygen sensing in polymer matrices and triplet-state lifetime mapping in metal-organic frameworks. Routine applications include excitation/emission matrix (EEM) analysis for complex mixtures, synchronous scanning for overlapping fluorophores, and pH-dependent fluorescence titrations requiring high photostability.

FAQ

Is this LS-45 unit factory-refurbished or dealer-refurbished?
This unit was refurbished by an ISO 9001-certified third-party service provider specializing in PerkinElmer instrumentation, following OEM technical bulletins and component replacement guidelines. All replaced parts carry full traceability.
Does the 6-month warranty cover lamp replacement?
Yes—the warranty includes labor and parts for the xenon lamp assembly, PMT detector, monochromator drive mechanisms, and all optomechanical subsystems under normal laboratory use conditions.
Can the LS-45 be upgraded to support time-resolved fluorescence (TRF)?
No—TRF requires hardware-level modifications (e.g., TCSPC module, fast-gated detector) not present in the LS-45 architecture. For TRF, consider the LS-55 or newer Lumina series.
Are original manuals and software installation media included?
Yes—each unit ships with printed operation manuals, calibration certificates, FL WinLab™ v4.00 installation DVD, and a valid software license key.
What documentation accompanies the instrument for regulatory submissions?
A complete Refurbishment Dossier is provided, including pre- and post-refurbishment test reports, calibration certificates traceable to NIST standards, firmware version log, and a Statement of Conformance to ISO/IEC 17025:2017 clause 6.4.