Persee T6 Used UV-Vis Spectrophotometer
| Brand | Persee |
|---|---|
| Origin | Beijing, China |
| Manufacturer Type | Authorized Distributor |
| Product Origin | Domestic (China) |
| Model | T6 |
| Price | USD 1,150 (FOB Beijing) |
| Optical Design | Double-beam |
| Detector | Photodiode Array (PDA) |
| Wavelength Range | 190–1100 nm |
| Wavelength Scanning | Motorized Auto-Scan |
| Spectral Bandwidth | 2 nm |
| Wavelength Accuracy | ±1.0 nm |
| Stray Light | ≤0.05 % |
Overview
The Persee T6 is a double-beam UV-Vis spectrophotometer engineered for high reproducibility and routine quantitative analysis in regulated and research laboratory environments. Based on classical optical absorption spectroscopy principles—where sample absorbance is measured according to the Beer-Lambert law—the T6 employs a stable tungsten-halogen lamp for visible/NIR wavelengths and a deuterium lamp for UV emission, enabling continuous spectral acquisition across 190–1100 nm. Its double-beam architecture compensates for source drift and detector instability in real time by simultaneously measuring reference and sample beams, significantly enhancing measurement robustness during extended batch analyses. The instrument integrates a motorized wavelength drive system for precise, programmable scanning and features a photodiode array (PDA) detector that captures full spectra without mechanical slit scanning—reducing mechanical wear and improving data acquisition speed and consistency.
Key Features
- Double-beam optical path with real-time reference compensation for enhanced baseline stability and long-term measurement repeatability
- Photodiode array (PDA) detector providing simultaneous multi-wavelength detection—eliminating moving grating mechanisms and minimizing maintenance intervals
- Motorized auto-wavelength scanning with ±1.0 nm accuracy and 2 nm spectral bandwidth compliant with ISO 9001 calibration traceability requirements
- Stray light performance ≤0.05 % at 220 nm (NaI) and 340 nm (NaNO₂), meeting ASTM E275 and USP specifications for pharmaceutical assay validation
- Integrated cuvette holder accommodating standard 10 mm pathlength cells; optional accessories include temperature-controlled holders and fiber-optic probes
- Firmware supports GLP-compliant operation including user access levels, audit trail logging (time-stamped events), and method password protection
Sample Compatibility & Compliance
The T6 accommodates liquid samples in quartz, glass, or plastic cuvettes (1–5 cm pathlength), as well as solid thin films via optional reflectance accessories. It is routinely deployed in QC laboratories performing raw material identification, dissolution testing, and stability-indicating assays per ICH Q2(R2) guidelines. Instrument design adheres to electromagnetic compatibility (EMC) standards IEC 61326-1 and safety requirements IEC 61010-1. While not pre-certified for FDA 21 CFR Part 11, its firmware architecture supports electronic signature implementation when integrated with validated LIMS or ELN platforms—enabling compliance-ready workflows under GMP/GLP environments.
Software & Data Management
The T6 operates with Persee’s proprietary UVWin software (v5.2+), compatible with Windows 10/11 64-bit systems. Software functions include kinetic scan, multi-wavelength quantitation, spectrum subtraction, derivative spectroscopy, and DNA/protein A280/A260 ratio calculation. All spectral data are stored in vendor-neutral .csv and .jdx formats; raw absorbance values are exportable without proprietary codec restrictions. Audit trail logs record operator ID, method name, timestamp, and parameter changes—supporting internal quality audits and regulatory inspections. Remote instrument control via LAN is supported for centralized lab management.
Applications
- Pharmaceutical QC: Active pharmaceutical ingredient (API) assay, excipient purity verification, and cleaning validation swab extract analysis
- Environmental monitoring: Nitrate/nitrite, phosphate, and COD determination in wastewater per EPA Method 353.2 and ISO 15681-1
- Food & beverage: Color index (e.g., AOAC 993.02), caffeine quantitation, and antioxidant capacity (FRAP/DPPH) assays
- Academic research: Ligand-binding studies, enzyme kinetics (initial rate analysis), and nanoparticle size estimation via surface plasmon resonance peak tracking
- Chemical manufacturing: Reaction endpoint monitoring, catalyst degradation profiling, and polymerization degree assessment
FAQ
Is the T6 suitable for GMP-regulated environments?
Yes—when operated with documented procedures, calibrated against NIST-traceable standards, and integrated into a validated data management workflow, the T6 meets core analytical instrument qualification (AIQ) requirements per Annex 15 and USP .
What is the typical recalibration interval?
Per ISO/IEC 17025 recommendations, wavelength and photometric accuracy should be verified quarterly using holmium oxide and potassium dichromate reference standards; full recalibration is advised annually or after major optical component replacement.
Can third-party software interface with the T6?
Direct OEM driver support is limited to UVWin; however, ASCII-based data export (.csv) enables integration with Python-based analysis pipelines, MATLAB scripts, or commercial ELN systems via automated file ingestion protocols.
Does the unit include a certificate of inspection?
Each refurbished T6 undergoes functional verification at the distributor’s ISO 9001-certified facility and ships with a Certificate of Conformance detailing lamp hours, detector response uniformity test results, and stray light verification data.
