Revvity LabChip GXII Touch Nucleic Acid and Protein Analysis System
| Brand | Revvity |
|---|---|
| Origin | USA |
| Manufacturer Type | Original Equipment Manufacturer (OEM) |
| Product Category | Imported Instrument |
| Model | LabChip GXII Touch 24 |
| Instrument Classification | Ultra-Micro Nucleic Acid and Protein Analyzer |
| Analysis Time per Sample | ≤ 41 seconds |
| Throughput | Up to 384 samples per run (batch processing, 1–384 scalable) |
| Detection Principle | Microfluidic Capillary Electrophoresis (CE) with Integrated Fluorescent Detection |
| Sample Volume Requirement | 1–2 µL |
| Compatible Assay Kits | Protein Express, DNA 1K/5K/10K, RNA Nano/Pico, GlycanExpress, Charge Variant, Fragment Analyzer kits |
| Regulatory Compliance Support | 21 CFR Part 11 compliant software architecture, audit trail, electronic signatures, and secure user access control |
Overview
The Revvity LabChip GXII Touch Nucleic Acid and Protein Analysis System is an automated, microfluidic capillary electrophoresis (CE)-based platform engineered for high-resolution, quantitative analysis of nucleic acids and proteins in life science research and biopharmaceutical development. Unlike conventional slab-gel electrophoresis, the GXII Touch replaces manual casting, staining, imaging, and densitometry with a fully integrated, chip-based workflow—delivering reproducible, digital electropherograms with calibrated sizing and quantitation. Its core technology leverages precisely fabricated glass microchips containing embedded separation channels, fluorescent dyes, and integrated detection optics, enabling rapid electrokinetic separation under controlled voltage gradients. Designed for seamless integration into QC, upstream/downstream process development, and analytical characterization workflows, the system supports both discovery-stage screening and regulated GMP environments where data integrity, traceability, and method robustness are critical.
Key Features
- Ultra-rapid analysis: Complete electrophoretic separation and quantitation in ≤ 41 seconds per sample—up to 70× faster than traditional CE systems and >100× faster than agarose or SDS-PAGE.
- Scalable throughput architecture: Process 1 to 384 samples per run using standardized 96-well or 384-well plate formats; no reconfiguration required between low- and high-throughput modes.
- Microfluidic chip-based operation: Pre-packaged, single-use LabChip assays eliminate gel preparation, buffer handling, and post-run staining—reducing hands-on time and inter-operator variability.
- Integrated touch interface and embedded computing: The GXII Touch features an intuitive, dedicated touchscreen console with onboard instrument control, real-time electropherogram visualization, and immediate pass/fail assessment against user-defined criteria.
- Multi-analyte versatility: Supports orthogonal assay chemistries—including Protein Express (reduced/non-reduced mAb purity, charge variants, fragmentation), DNA 1K/5K/10K (size distribution, integrity), RNA Nano/Pico (RIN-equivalent metrics), and GlycanExpress (N-glycan profiling).
- Regulatory-ready software: Compliant with FDA 21 CFR Part 11 requirements via secure login, role-based permissions, full electronic audit trail, and tamper-evident data archiving.
Sample Compatibility & Compliance
The LabChip GXII Touch accommodates diverse biological samples without dilution or cleanup: clarified cell lysates, purified monoclonal antibodies, in-process fermentation broths, fragmented genomic DNA, total RNA isolates, and enzymatically digested glycoproteins. All assays are validated per ICH Q5E and aligned with USP , ASTM E2915, and ISO/IEC 17025 principles for method verification. Each chip lot undergoes rigorous release testing for migration time precision (RSD < 2%), peak area reproducibility (RSD < 5%), and sizing accuracy (±5% vs. ladder standards). The platform is routinely deployed in GLP-compliant labs and supports formal validation protocols for IQ/OQ/PQ execution.
Software & Data Management
The GXII Touch runs on Revvity’s proprietary ChipPal software—designed for both technical users and non-specialists. It provides automated baseline correction, peak calling, molecular weight calibration, concentration calculation (via internal standards), and customizable reporting templates (PDF, CSV, Excel). Raw data (.lch files) and processed results are stored in a relational database with version-controlled metadata, including instrument parameters, chip lot ID, operator ID, and timestamped environmental logs. Data export supports LIMS integration via HL7 or RESTful API, and raw electropherograms are compatible with third-party analysis tools such as PeakFit and SoftMax Pro for advanced statistical modeling.
Applications
- Biopharmaceutical characterization: mAb purity assessment (main peak, aggregates, fragments), charge variant profiling (CEX-like resolution), and forced degradation monitoring.
- Nucleic acid quality control: dsDNA integrity screening (e.g., plasmid supercoiling, shearing), RNA integrity scoring (analogous to RIN), and NGS library QC (insert size distribution, adapter dimer detection).
- Cell and gene therapy analytics: AAV capsid protein ratio (VP1/VP2/VP3), lentiviral vector genome integrity, and CRISPR gRNA cleavage efficiency quantification.
- Upstream process development: Rapid titer estimation from harvest supernatants, clone selection based on expression profile consistency, and media optimization feedback loops.
- Downstream purification monitoring: Real-time assessment of column elution fractions for product-related impurities and host cell protein clearance.
FAQ
What types of samples can be analyzed without modification?
Clarified mammalian cell culture supernatants, purified IgGs, crude lysates (with optional dilution), and standard nucleic acid preps (e.g., Qiagen columns, SPRI beads) are directly compatible—no desalting or buffer exchange required.
Is method transfer from traditional CE or SDS-PAGE feasible?
Yes—Revvity provides cross-platform correlation guides and application notes demonstrating equivalence in sizing resolution (≤ 2% CV for fragment sizing) and quantitation linearity (R² > 0.999 over 3-log range).
How is data integrity maintained during routine use?
All user actions, parameter changes, and result exports are logged with immutable timestamps and operator credentials; audit trails are exportable in PDF/A format for regulatory submission.
Are LabChip assays supplied sterile and endotoxin-tested?
Yes—each chip batch is certified for sterility (USP ) and endotoxin levels (<0.5 EU/mL), supporting use in preclinical and clinical manufacturing environments.
Can the GXII Touch be integrated into automated liquid handling workflows?
Absolutely—the system accepts standard SBS-format plates and supports robotic deck integration via RS-232 or Ethernet command protocol; Revvity offers validated interfaces for Hamilton STAR, Tecan Freedom EVO, and Agilent Bravo platforms.
