RWD SP0008-M Mouse Cerebrovascular and Cardiac Perfusion Surgical Instrument Set
| Brand | RWD |
|---|---|
| Origin | Guangdong, China |
| Manufacturer Type | OEM/ODM Manufacturer |
| Country of Origin | China |
| Model | SP0008-M |
| Pricing | Available Upon Request |
Overview
The RWD SP0008-M Mouse Cerebrovascular and Cardiac Perfusion Surgical Instrument Set is a purpose-engineered, sterile-ready collection of microsurgical tools designed specifically for high-precision intracardiac and transcranial perfusion procedures in murine models. This set supports standardized protocols for terminal perfusion fixation—commonly employed prior to histology, immunohistochemistry, or ex vivo brain/cardiopulmonary tissue harvesting. All instruments are fabricated from medical-grade stainless steel (AISI 420 or equivalent), heat-treated for optimal hardness (52–56 HRC), and electropolished to minimize tissue adhesion and ensure cleanability under GLP-compliant laboratory conditions. The ergonomic design prioritizes tactile feedback and control during delicate vascular access (e.g., left ventricle cannulation) and dural exposure, enabling reproducible outcomes across longitudinal studies involving transgenic, knockout, or disease-model mice (C57BL/6, BALB/c, CD-1, etc.).
Key Features
- Comprehensive microsurgical coverage: Includes dedicated instruments for thoracotomy, cardiac puncture, cranial trephination, dural incision, and cerebral vasculature manipulation.
- Precision-calibrated fine-cutting tools: IRIS-Fine scissors (9.5 cm) and VANNAS spring scissors (8.5 cm) feature hand-forged tungsten-carbide inserts for consistent edge retention during repeated use on neural and vascular tissues.
- Optimized grasping and stabilization: Bent ophthalmic forceps (0.8 mm tip width) enable controlled handling of pial vessels; straight anatomical forceps (1.8 mm tip) provide secure tissue retraction without slippage.
- Controlled hemostasis: Straight and curved Hartmann mosquito clamps (1.0 mm jaw width) deliver predictable compression to capillary-level bleeders without vessel wall damage.
- Integrated suture system: Pre-sterilized PGA absorbable sutures (5-0, ½-circle needle) and non-absorbable nylon sutures (6-0, 3/8-circle triangular needle) meet ASTM F1871 and ISO 10993-1 biocompatibility requirements for in vivo use.
- Specialized auxiliary tools: Stevens single-tooth retractor (90° bend, 5 mm tooth), Myrtle probe (2.0 mm diameter), and spinal retractor (3 mm blade) facilitate controlled dural elevation and cerebellar exposure with minimal parenchymal displacement.
Sample Compatibility & Compliance
This instrument set is validated for use in adult and juvenile mice (18–35 g body weight), including strains with elevated intracranial pressure or compromised cardiovascular function. All components comply with ISO 7153-1:2016 (surgical instruments — stainless steels) and are autoclavable at 134 °C (273 °F) for 18 minutes per cycle. Instruments meet EU MDR 2017/745 Class I sterility requirements when supplied in ISO 11607-compliant packaging. The set supports adherence to NIH Guide for the Care and Use of Laboratory Animals and ARRIVE 2.0 reporting standards. No animal-derived materials are used in manufacturing; all tools are free of latex, phthalates, and endotoxin (<0.5 EU/mL per USP ).
Software & Data Management
While this is a manual surgical toolkit (no embedded electronics), RWD provides digital documentation support via its LabManager™ Companion Portal. Users gain access to downloadable SOP templates (including step-by-step perfusion workflows), instrument traceability logs (batch-specific sterilization records), and video-guided technique modules aligned with common endpoints: Evans Blue extravasation assessment, fluorescent tracer distribution mapping, and post-perfusion tissue integrity scoring. Audit trails generated through the portal satisfy internal GLP audit requirements and align with FDA 21 CFR Part 11 principles for electronic record integrity.
Applications
- Transcardiac perfusion with paraformaldehyde or glutaraldehyde for electron microscopy or light microscopy sample preparation.
- Fluorescent or enzymatic tracer delivery (e.g., FITC-dextran, lectin, or horseradish peroxidase) to assess blood–brain barrier integrity.
- Ex vivo functional assays requiring intact cerebral vasculature (e.g., pressurized myography of pial arterioles).
- Cardiac perfusion for Langendorff preparation or isolated heart analysis.
- Combined neurovascular modeling: simultaneous carotid ligation + intracardiac perfusion in stroke or Alzheimer’s disease models.
FAQ
Is this set compatible with rats or larger rodents?
No. The SP0008-M is dimensionally optimized for mice only. For rat applications, refer to the RWD SP0009-R Rat Perfusion Set (designed for 200–300 g Sprague-Dawley or Wistar subjects).
Are replacement instruments available individually?
Yes. All components—including stainless steel microvascular clamps (R31005-04), Olsen-Hegar needle holders (F31047-12), and PGA suture packs (F35305-50)—are stocked as standalone SKUs with full traceability documentation.
Can the set be resterilized after use?
Yes. All instruments are reusable and withstand ≥50 autoclave cycles per ISO 17664. We recommend ultrasonic cleaning (2% enzymatic detergent, 40 kHz, 10 min) followed by steam sterilization at 134 °C.
Does RWD offer training for perfusion techniques?
Yes. On-site and virtual hands-on workshops are available upon request, covering mouse positioning, thoracotomy depth control, cannula placement verification, and troubleshooting common flow resistance issues.
