Sida SP-64A Solid Phase Microextraction Instrument

Overview

The Sida SP-64A Solid Phase Microextraction (SPME) Instrument is an automated, high-throughput sample preparation platform engineered for reproducible and solvent-free extraction of volatile and semi-volatile organic compounds (VOCs and SVOCs) from liquid or headspace matrices. Based on the principle of equilibrium partitioning between a coated fused-silica fiber and the sample phase, the SP-64A enables direct, in-situ enrichment without liquid–liquid extraction or derivatization. It is specifically designed to interface seamlessly with gas chromatography (GC) and GC–mass spectrometry (GC–MS) systems via a dedicated, non-invasive inlet adapter—eliminating competition for the analytical instrument’s injection port. The system supports both static and dynamic (agitated) headspace modes, as well as immersion extraction, making it suitable for method development and routine compliance testing across environmental, clinical, forensic, pharmaceutical, and public health laboratories.

Key Features

• Automated 64-position sample handling with precise robotic arm positioning and thermal control for consistent fiber exposure timing and temperature.
• Integrated programmable heating block (ambient to 150 °C) and magnetic stirring for accelerated mass transfer during immersion or headspace equilibration.
• Quick-connect modular interface compatible with standard GC autosampler trays; no modification to existing GC/MS hardware required.
• Dedicated high-temperature conditioning port (up to 320 °C) for on-instrument fiber aging and nitrogen purging—ensuring baseline stability and minimizing carryover.
• PC-based control software with role-based user authentication, audit trail logging, and full method parameter scripting (extraction time, temperature, agitation speed, desorption duration).
• Compliant with data integrity requirements for regulated environments: supports electronic signatures, session lockout, and configurable retention of method files and run logs.

Sample Compatibility & Compliance

The SP-64A accommodates standard 20 mL crimp-top headspace vials and is validated for use with common SPME fiber coatings including PDMS, DVB/CAR/PDMS, and CW/DVB. Its operational parameters align with internationally recognized standard methods, including GB/T 5750.8–2023 “Standard Examination Methods for Drinking Water – Odor-Related Compounds”, where it facilitates quantitative analysis of geosmin and 2-methylisoborneol (2-MIB) by headspace SPME–GC–MS. The system architecture supports GLP and GMP-aligned workflows, and its software design incorporates features traceable to FDA 21 CFR Part 11 expectations—including secure user access, immutable audit trails, and electronic record retention protocols.

Software & Data Management

The instrument is operated via SidaControl™ v3.x, a Windows-based application supporting method creation, sequence building, real-time status monitoring, and post-run report generation. All user actions—including login/logout, method edits, and run initiation—are timestamped and logged with operator ID. Raw method files (.spm) and execution logs are stored in encrypted directories with configurable backup intervals. Export options include CSV and PDF formats compliant with LIMS integration requirements. Software validation documentation (IQ/OQ templates) is available upon request for laboratory qualification processes.

Applications

• Environmental monitoring: quantification of earthy–musty odorants (geosmin, 2-MIB) in source and finished drinking water per GB/T 5750.8–2023.
• Forensic toxicology: extraction of volatile drugs of abuse and accelerants from biological or fire debris samples.
• Pharmaceutical quality control: residual solvent analysis in active pharmaceutical ingredients (APIs) and excipients.
• Clinical research: headspace profiling of volatile biomarkers in urine, blood headspace, or breath condensate.
• Food and fragrance analysis: characterization of aroma-active compounds in beverages, dairy, and essential oils.

FAQ

Does the SP-64A require modification to my existing GC or GC–MS system?
No—the instrument uses a standalone autosampler-style interface and connects externally to the GC inlet via a dedicated SPME introduction liner; no hardware retrofitting is needed.
Can I use third-party SPME fibers with this instrument?
Yes—it accepts all standard 1-cm or 2-cm length SPME fibers with 24-gauge stainless steel holders, regardless of coating or vendor.
Is method validation support provided?
Sida supplies application notes, system suitability test procedures, and reference data for GB/T 5750.8–2023 compliance; full method validation packages are available under NDA.
What maintenance is required for long-term reliability?
Routine tasks include periodic cleaning of the fiber needle guide, verification of vial tray alignment, and annual calibration of the heating block and temperature sensor using NIST-traceable references.
Is remote operation supported?
Yes—via secure RDP or VPN connection to the host PC; however, local user presence is recommended during fiber insertion and system startup sequences.