Supelco KLVIPSPE-24 24-Position Manual Solid Phase Extraction Manifold

Overview

The Supelco KLVIPSPE-24 is a manually operated, 24-position solid phase extraction (SPE) manifold engineered for reproducible, high-throughput sample preparation in analytical laboratories. It operates on the fundamental principle of selective adsorption and elution—where analytes are retained on a solid sorbent packed in disposable cartridges or columns, followed by sequential washing and targeted elution using controlled solvent gradients. Designed for compatibility with standard 1–60 mL SPE cartridges—including C18, silica, Florisil®, ion-exchange, and mixed-mode phases—the system supports method development and routine QC workflows across environmental, pharmaceutical, food safety, and clinical research applications. Its modular architecture integrates seamlessly with vacuum sources (e.g., oil-free diaphragm pumps rated ≥25 L/min) and enables precise flow regulation without reliance on electronic controllers—ensuring robustness under variable solvent conditions and minimizing operator-induced variability.

Key Features

• 24-channel parallel processing capability for improved throughput without compromising method integrity.
• Visiprep™ DL design with borosilicate glass reservoir: chemically inert, non-fogging, and optically transparent for real-time monitoring of solvent flow and cartridge conditioning.
• Patented spiral-flow control valves: provide tactile, stepwise adjustment of per-channel flow rates—critical for optimizing retention, wash efficiency, and elution recovery across diverse matrix types.
• Disposable PTFE-Luer connection tubing (included): eliminates cross-contamination between samples by enabling full replacement of fluidic pathways prior to each run—validated for use in GLP-compliant residue analysis and regulated bioanalytical workflows.
• Vacuum manifold with integrated solvent-resistant pressure gauge and sealed 1/4″ barbed port: ensures stable vacuum delivery while preventing backstreaming of vapors or condensates into the pump.
• Modular polypropylene collection rack: accommodates industry-standard containers including 1–10 mL conical vials, 10/16 mm round-bottom tubes, 20 mL scintillation vials, and autosampler-ready vials—supporting direct transfer to HPLC, GC, or LC-MS instrumentation.

Sample Compatibility & Compliance

The KLVIPSPE-24 is compatible with all commercially available SPE cartridges (1–60 mL formats) and supports method protocols aligned with ASTM D7237 (pesticide residue analysis), ISO 17025-accredited validation requirements, and USP chromatographic system suitability guidelines. All wetted components—including the glass chamber, PTFE valves, and Luer-tipped tubing—exhibit full resistance to aggressive solvents (e.g., dichloromethane, THF, concentrated acids/bases) and maintain dimensional stability over repeated thermal and chemical cycling. The system meets essential criteria for FDA 21 CFR Part 11 readiness when paired with validated electronic logbooks and audit-trail-enabled vacuum controllers (sold separately). No metal components contact solvents, eliminating leaching risks in trace-metal or elemental speciation workflows.

Software & Data Management

As a manual manifold, the KLVIPSPE-24 does not incorporate embedded firmware or proprietary software. However, its mechanical design supports integration into fully documented laboratory workflows through external SOP-driven documentation systems. Users routinely pair it with LIMS platforms (e.g., LabWare, STARLIMS) via standardized sample tracking templates. Optional accessories—including large-volume samplers (models 51719, 51688) and buffer bottles (51153)—are assigned unique catalog identifiers traceable to Supelco’s certified reference material database. All consumables (e.g., PTFE connection tubes, part #51598) carry lot-specific CoA documentation compliant with ISO/IEC 17025 calibration traceability requirements.

Applications

• Environmental analysis: isolation of PAHs, PCBs, pesticides, and pharmaceuticals from wastewater, soil extracts, and air particulate filters.
• Pharmaceutical QC: cleanup of plasma, urine, and tissue homogenates prior to LC-MS/MS quantitation of APIs and metabolites.
• Food safety testing: removal of lipids, pigments, and organic acids from milk, honey, and fruit juice matrices before multi-residue pesticide screening.
• Clinical toxicology: selective enrichment of opioids, benzodiazepines, and stimulants from biological fluids under CLIA-waived or CAP-accredited protocols.
• Forensic chemistry: fractionation of complex post-mortem blood extracts for targeted confirmation assays.

FAQ

Is the KLVIPSPE-24 compatible with 60 mL SPE cartridges?
Yes—it accepts standard 1–60 mL cartridge formats using optional adapter sleeves (part #51105) for dual-cartridge stacking or oversized column mounting.
What vacuum pump specifications are recommended?
An oil-free diaphragm pump with ≥25 L/min free-air displacement and ultimate vacuum ≤80 mbar is required; Supelco recommends part #501-2001 for optimal solvent vapor handling.
How is cross-contamination prevented during sequential runs?
By replacing the PTFE-Luer connection tubing (part #51598) before each new batch—this physically isolates fluid paths and eliminates residual carryover, a practice verified in AOAC 2012.01 validation studies.
Can the manifold be used under nitrogen purge instead of vacuum?
No—it is designed exclusively for negative-pressure operation; positive-pressure (nitrogen-driven) SPE requires dedicated positive-pressure manifolds (e.g., Supelco KLVIPSPEN series).
Are replacement glass chambers available as spare parts?
Yes—borosilicate glass reservoirs are supplied under part #51680-GCH and include calibrated volume markings for consistent solvent addition across batches.