Taicang Qiangle Beijing HZL-F160 Dual-Deck Full-Temperature Orbital Shaker

Overview

The Taicang Qiangle Beijing HZL-F160 is a dual-deck, full-temperature orbital shaker engineered for reproducible, long-duration cultivation of microbial, mammalian, and insect cell cultures under tightly controlled thermal and mechanical conditions. Operating on the principle of orbital motion—where the platform rotates in a horizontal circular path with a fixed 26 mm diameter—the instrument generates gentle yet uniform mixing ideal for aerobic suspension cultures, enzyme assays, solubility studies, and biochemical incubation protocols. Unlike reciprocating or vortex shakers, orbital agitation minimizes shear stress on sensitive cells while ensuring consistent oxygen transfer across large-volume flask arrays. Its integrated refrigeration-heating system enables stable temperature maintenance from 5 °C to 60 °C (measured at ambient 25 °C), making it suitable for psychrophilic strain propagation, mesophilic fermentation, and thermophilic expression studies—all within a single platform.

Key Features

• Dual independent decks: Two vertically stacked, motor-driven platforms allow simultaneous operation at identical or distinct oscillation parameters—enabling parallel experiments with differentiated culture conditions without cross-contamination risk.
• Precision microprocessor control: A dedicated embedded controller manages real-time regulation of temperature, speed, and timer functions; all parameters are displayed via high-contrast LED interface with tactile keypad navigation.
• Brushless DC motor drive: Delivers quiet, maintenance-free operation with high torque consistency across the full 40–280 rpm range and exceptional speed stability (< ±1 rpm deviation under load).
• High-accuracy thermal management: PID-controlled Peltier-assisted heating/cooling system achieves ±0.1 °C temperature uniformity across the entire 160 L chamber volume, verified per ASTM E2777-21 guidelines for laboratory incubator performance validation.
• Robust mechanical architecture: Reinforced steel frame with vibration-dampening feet ensures structural integrity during extended operation; stainless-steel shelves support standard Erlenmeyer flasks (up to 250 mL × 40 per deck) and accommodate custom clamping accessories.

Sample Compatibility & Compliance

The HZL-F160 accommodates standard microbiological and bioprocessing vessels—including glass and polycarbonate flasks (50–500 mL), baffled shake flasks, and multi-well plates mounted on universal adapters. Its 160 L internal volume supports scalable culture workflows compliant with ISO 13408-2 (aseptic processing of pharmaceuticals) and USP (cell culture performance qualification). The device meets IEC 61010-1:2010 safety requirements for laboratory equipment and carries CE marking for EMC and low-voltage directives. While not certified for GMP production environments, its audit-ready parameter logging capability (via optional RS-232/USB output) supports GLP-compliant documentation per FDA 21 CFR Part 11 when paired with validated third-party data acquisition software.

Software & Data Management

The HZL-F160 operates autonomously via onboard firmware but supports external data capture through its serial communication port. When connected to a host PC running validated laboratory information management system (LIMS) clients or custom Python/Python-based scripts (e.g., PySerial), users may log timestamped records of setpoints, actual temperatures, rotational speeds, and elapsed time. All operational events—including power interruptions, door openings (if equipped with optional safety interlock), and parameter changes—are stored in non-volatile memory for post-run forensic analysis. Firmware updates are delivered via USB flash drive following secure SHA-256 checksum verification.

Applications

• Microbial growth kinetics: Standardized cultivation of E. coli, Bacillus subtilis, and yeast strains under defined aeration and thermal profiles.
• Recombinant protein expression: Optimization of induction timing, temperature shifts, and shaking intensity for soluble target yield in pET-based systems.
• Antibiotic susceptibility testing: Agitation-enhanced broth microdilution assays per CLSI M07-A11 standards.
• Enzyme activity screening: Homogeneous mixing during kinetic assays involving NAD(P)H-coupled reactions or chromogenic substrates.
• Cell line expansion: Gentle suspension of CHO or HEK293 cells prior to bioreactor inoculation, minimizing aggregate formation.

FAQ

Does the HZL-F160 support remote monitoring or Ethernet connectivity?
No—this model lacks built-in network interfaces. Remote supervision requires integration with external IoT gateways or programmable logic controllers via its analog/digital I/O expansion port (optional accessory).
Can both decks operate at different temperatures simultaneously?
No—temperature is regulated uniformly across the entire chamber; independent deck-level thermal control is not supported.
What is the maximum flask fill volume recommended at 280 rpm?
For optimal oxygen transfer and minimal splashing, maintain working volumes at ≤30% of flask capacity (e.g., ≤75 mL in 250 mL flasks) when operating above 200 rpm.
Is calibration documentation provided with shipment?
A factory-issued temperature and speed verification report (traceable to NIM China national standards) is included; user-performed periodic recalibration is advised every 6 months per ISO/IEC 17025:2017 clause 7.7.
Are replacement shelves or custom clamping kits available?
Yes—Taicang Qiangle offers OEM-compatible stainless-steel shelves, spring-loaded universal clamps, and microplate carriers (96-/384-well) under part numbers QL-SH-260, QL-CL-UNI, and QL-MP-96 respectively.