Xiangyi H2500R-2 High-Capacity Refrigerated High-Speed Centrifuge

Overview

The Xiangyi H2500R-2 is a floor-standing, refrigerated high-speed centrifuge engineered for reproducible, high-throughput separation of biomolecular samples under controlled thermal conditions. Operating on the principle of sedimentation force generation via rotational acceleration, it delivers precise RCF (Relative Centrifugal Force) control up to 64,800 × g at 25,000 rpm—enabling efficient pelleting of subcellular organelles, nucleic acids, viral particles, and macromolecular complexes. Its integrated refrigeration system maintains sample temperature between –20 °C and +40 °C during operation, minimizing thermal degradation of heat-sensitive analytes such as enzymes, antibodies, and RNA. Designed for compliance with routine laboratory workflows in academic research, biopharmaceutical development, and clinical diagnostics, the H2500R-2 meets structural and operational expectations aligned with ISO 13485-informed design practices and supports GLP/GMP-aligned documentation requirements through comprehensive audit-trail-capable data logging.

Key Features

• Direct-drive inverter motor with high starting torque ensures rapid acceleration (<30 s to 25,000 rpm) and stable speed regulation with ±10 rpm speed accuracy.
• 7-inch capacitive touchscreen interface with multi-language support (English, German, French, Spanish, Chinese) enables intuitive parameter entry, real-time monitoring of RCF, temperature, time, and rotor status—all displayed simultaneously.
• Automatic rotor recognition technology reads embedded RFID tags or mechanical coding to validate rotor identity, maximum speed rating, and safety limits prior to run initiation.
• Comprehensive safety architecture includes active imbalance detection (vibration amplitude thresholding), overtemperature shutdown (compressor + chamber sensors), overspeed cutoff, and door interlock verification.
• Forged 7075-T6 aluminum alloy rotors undergo hard anodization (≥25 µm oxide layer thickness) for enhanced corrosion resistance, mechanical durability, and long service life—validated per ASTM B580 Class II specifications.
• Onboard non-volatile memory stores ≥6 months of runtime logs, including start/stop timestamps, setpoints, fault codes, and environmental deviations—exportable via USB for traceability and internal QA review.

Sample Compatibility & Compliance

The H2500R-2 accommodates a broad range of sample formats via 13 interchangeable rotor configurations—including horizontal swing-out, fixed-angle, and continuous-flow variants—supporting volumes from 0.2 mL microtubes to 4 × 1000 mL bottles. It is routinely deployed in protocols compliant with ISO 15189 (medical laboratories), USP (analytical instrument qualification), and FDA 21 CFR Part 11 (electronic records and signatures) when paired with validated software extensions. All rotor geometries are certified for use with standard polypropylene, polycarbonate, and stainless-steel tubes meeting IEC 61010-2-020 safety standards. The unit operates within ambient temperature ranges of 10–32 °C and relative humidity ≤80% non-condensing, fulfilling EN 61326-1 electromagnetic compatibility requirements.

Software & Data Management

The embedded control firmware supports programmable multi-step runs with up to 99 segments per method, including ramp/hold profiles, temperature ramping, and deceleration braking control. Users may define and annotate >100 custom programs with descriptive metadata (e.g., “Plasma Exosome Isolation – Protocol v2.1 – Validated 2024”). Data export is supported via USB 2.0 port in CSV format, compatible with LIMS integration and statistical process control platforms. Optional PC-based software (Xiangyi CentriSoft v3.x) provides remote monitoring, electronic signature capture, and automated report generation aligned with ALCOA+ principles (Attributable, Legible, Contemporaneous, Original, Accurate, Complete, Consistent, Enduring, Available).

Applications

• Isolation of extracellular vesicles (exosomes, microvesicles) from serum, plasma, and cell culture supernatants.
• Purification of plasmid DNA, genomic DNA, and total RNA using density gradient or pelleting protocols.
• Subcellular fractionation of mitochondria, lysosomes, and nuclei from tissue homogenates.
• Clarification and concentration of monoclonal antibodies and recombinant proteins in upstream bioprocessing.
• Virus harvesting and purification in vaccine development and virology research.
• High-volume processing of blood-derived products (e.g., platelet-rich plasma, cryoprecipitate) in transfusion medicine labs.

FAQ

What is the minimum operating temperature of the H2500R-2?

The refrigeration system achieves and maintains a chamber temperature as low as –20 °C at ambient conditions ≤25 °C.
Does the H2500R-2 support validation documentation for GxP environments?

Yes—the instrument’s firmware and optional CentriSoft software provide IQ/OQ templates, calibration traceability logs, and electronic audit trails meeting FDA 21 CFR Part 11 and EU Annex 11 expectations.
Can third-party rotors be used with this centrifuge?

No. Only Xiangyi-certified rotors with embedded identification are permitted; the system blocks operation if unrecognized or mismatched rotor parameters are detected.
How is rotor lifetime tracked and managed?

Each rotor’s cumulative run time, maximum speed history, and thermal cycling count are logged automatically; alerts trigger when usage thresholds approach manufacturer-specified service intervals.
Is remote monitoring or network connectivity available?

Standard configuration includes USB data export only; Ethernet or Wi-Fi modules are available as factory-installed options upon request for centralized lab management systems.