Yiheng BPN-100CS / BPN-300CS CO₂ Incubator Shaker

Overview

The Yiheng BPN-100CS and BPN-300CS CO₂ Incubator Shakers integrate precise environmental control with orbital shaking functionality in a single compact platform—engineered for mammalian cell culture, suspension bioreactor development, and microbiological assays requiring simultaneous CO₂ regulation, temperature stability, and mechanical agitation. These units operate on the principle of infrared-based CO₂ monitoring coupled with PID-controlled heating, humidification, and gas blending (optional O₂/N₂), while the shaker mechanism employs a three-eccentric-axis balanced drive system to ensure uniform motion distribution across the entire platform—even at maximum load and speed. Designed for GLP-compliant laboratories, the system maintains ISO 14644-1 Class 100 (ISO Class 5) internal air quality within five minutes post-door closure, supported by integrated HEPA filtration and high-efficiency microbial gas filtration. The chamber’s electrochemically passivated 304 stainless steel construction—featuring radiused corners and non-porous surfaces—minimizes microbial adhesion and facilitates validated cleaning and sterilization protocols.

Key Features

• Integrated CO₂ incubation and orbital shaking in one footprint—optimized for space-constrained labs without compromising capacity (100 L or 300 L working volume).
• 7-inch full-color capacitive touchscreen with multi-level password protection, real-time parameter dashboard (temperature, CO₂, O₂, RPM, humidity), and programmable timer functions.
• Imported NDIR (Non-Dispersive Infrared) CO₂ sensor offering ±0.1% accuracy, immunity to ambient humidity/temperature drift, and stable setpoint maintenance during frequent door openings.
• Zirconia-based electrochemical O₂ sensor (optional)—linear response across 0.1–90% O₂ range—enabling hypoxic, normoxic, and hyperoxic culture conditions within a single instrument.
• Dual-stage gas filtration: (1) 0.3 µm-rated microbial filter (99.99% efficiency) on CO₂ inlet; (2) Internal HEPA H14 filter (≥99.995% @ 0.3 µm) ensuring rapid recovery to ISO Class 5 air cleanliness.
• Door-heating system prevents condensation on inner glass, maintaining optical clarity and eliminating moisture-mediated contamination pathways.
• 90°C steam-saturated hot-humid sterilization cycle—fully automated, covering all internal components including sensors, fans, shelves, and brackets—validated for mycoplasma, bacterial, and fungal decontamination.
• Low-heat DC motor with high starting torque and wide-speed range (20–300 rpm), enabling uninterrupted operation over extended durations (>72 h) without thermal degradation or brush wear.

Sample Compatibility & Compliance

The BPN-series accommodates standard cell culture vessels—including T-flasks (up to 175 cm²), roller bottles, multi-well plates (6–96-well), Erlenmeyer flasks (50–5000 mL), and custom bioreactor bags—via interchangeable platforms and universal clamping systems. All wetted materials comply with USP Class VI biocompatibility standards. The unit meets IEC 61010-1 safety requirements for laboratory equipment and supports audit-ready documentation per FDA 21 CFR Part 11 when configured with optional分级权限 management (multi-tier user accounts, electronic signatures, and immutable operation logs). Environmental monitoring is enhanced by an independent ambient temperature sensor that dynamically adjusts heater output to prevent thermal overshoot—a critical feature for sensitive primary cell lines.

Software & Data Management

The embedded controller records time-stamped data for temperature, CO₂, O₂ (if installed), humidity, RPM, door status, alarm events, and sterilization cycles. Data export is supported via USB port in CSV format for traceability and integration into LIMS or ELN systems. Optional wireless SMS alerting transmits fault codes (e.g., CO₂ depletion, temperature deviation, door-open timeout >60 s) directly to designated mobile devices. The system supports configurable audit trails—including user login/logout timestamps, parameter changes, and sterilization initiation—with tamper-proof storage compliant with ALCOA+ principles (Attributable, Legible, Contemporaneous, Original, Accurate, Complete, Consistent, Enduring, Available).

Applications

• Adherent and suspension mammalian cell expansion under controlled CO₂/O₂ tension for monoclonal antibody production.
• Hypoxia modeling in cancer research using tunable O₂ setpoints (e.g., 1–5% O₂ for HIF-1α stabilization).
• Microbial fermentation optimization where dissolved CO₂ influences pH and metabolic flux (e.g., E. coli, S. cerevisiae).
• Stem cell differentiation studies requiring dynamic mechanical stimulation combined with gas-regulated microenvironments.
• Quality control testing of biologics under accelerated stress conditions (e.g., elevated CO₂ + agitation to assess product aggregation).
• Regulatory submissions requiring documented environmental consistency—supporting ISO 13485, GMP Annex 1, and USP ancillary equipment qualification.

FAQ

What CO₂ concentration range does the system support?
The standard configuration maintains 0–20% CO₂ with ±0.1% setpoint accuracy; calibration is traceable to NIST-certified gas standards.
Is humidification integrated, and what is the typical RH range?
Yes—passive humidification achieves 90–95% RH at 37°C; external humidification modules are available for extended low-temperature applications.
Can the unit be validated for GMP use?
Fully—IQ/OQ protocols are provided; PQ support includes thermocouple mapping, CO₂ uniformity testing (ASTM F2622), and HEPA integrity verification (EN 1822).
What safety interlocks are active during door opening?
Shaking stops immediately; heating and fan circulation are disabled; CO₂ injection halts; UV indicator activates if equipped.
Are third-party service contracts available?
Yes—Yiheng offers certified field service, preventive maintenance plans, and remote diagnostics with SLA-backed response times.