YiHeng LRH-500F Biochemical Incubator

Overview

The YiHeng LRH-500F Biochemical Incubator is an air-jacketed, compressor-cooled environmental chamber engineered for stable, long-term cultivation of microorganisms, cell lines, and biochemical samples under precisely controlled temperature conditions. Designed in accordance with ISO 13485-aligned manufacturing practices and aligned with GLP-compliant laboratory workflows, the LRH-500F operates across a broad temperature range (0–60 °C), supporting applications from psychrophilic bacterial growth to enzyme activity assays and shelf-life stability testing. Its air-jacketed heating architecture ensures rapid thermal response and minimal internal gradient formation, while the sealed refrigeration circuit—utilizing R134a or R600a refrigerant—enables consistent sub-ambient cooling without condensation accumulation in the chamber. Natural convection eliminates mechanical airflow-induced desiccation, preserving sample integrity during extended incubation periods.

Key Features

• Mirror-finish stainless steel interior with seamless, radius-corner construction—resistant to corrosion, non-porous, and compliant with ISO 8573-1 Class 3 cleanliness standards for repeated sterilization cycles.
• High-efficiency hermetic compressor system integrated with environmentally compliant refrigerant (R134a/R600a), delivering energy consumption reduction of ≥25% compared to conventional foam-insulated units per IEC 62304 Annex C benchmarking.
• Monolithic polyurethane foam insulation (≥80 mm thickness) applied via high-pressure foaming process—achieving thermal conductivity ≤0.022 W/m·K and minimizing heat exchange across all six chamber surfaces.
• Microprocessor-based PID temperature controller with 0.1 °C resolution, programmable ramp-hold profiles, and user-defined timer function (1 min to 999 hours).
• Three-stage adjustable circulation fan (off/low/medium)—optimized to maintain laminar ambient equilibrium without inducing evaporative loss in open petri dishes or liquid cultures.
• Dual-limit independent overtemperature protection circuit (mechanical + electronic), automatically cutting power when deviation exceeds preset safety thresholds—certified to IEC 61000-4-2 ESD immunity Level 3.

Sample Compatibility & Compliance

The LRH-500F accommodates standard microbiological and biochemical sample formats including Petri dishes (up to 150 mm diameter), multi-well plates (6–96-well), Erlenmeyer flasks (up to 2 L), and test tube racks. Its natural convection design prevents cross-contamination between stacked shelves and avoids shear stress on delicate suspension cultures. The unit complies with key regulatory frameworks applicable to lab equipment validation: EN 61010-1 (safety), EN 61326-1 (EMC), and supports IQ/OQ documentation packages for GMP environments. Optional RS485 Modbus RTU interface enables integration into centralized LabVantage or Thermo Fisher SampleManager LIMS systems with full audit trail capability per FDA 21 CFR Part 11 requirements.

Software & Data Management

Equipped with a backlit LCD interface featuring intuitive menu navigation and real-time parameter display (setpoint, actual temperature, elapsed time, alarm status), the LRH-500F supports optional data logging via built-in RS485 port. When connected to external PCs or industrial PLCs, it transmits timestamped temperature readings at configurable intervals (1–60 min), generating CSV-compatible logs suitable for statistical process control (SPC) analysis. Optional thermal printer module provides hard-copy traceability for batch-critical operations—fully compatible with ISO/IEC 17025 accredited calibration records.

Applications

• Bacterial and fungal culture maintenance (e.g., E. coli, S. aureus, A. niger) per CLSI M07-A10 guidelines.
• Enzyme kinetics studies requiring isothermal stability at 4 °C, 25 °C, 37 °C, or 55 °C.
• Pharmaceutical stability testing (ICH Q1A–Q1E) for accelerated and intermediate condition storage.
• Environmental simulation for biodegradation assays and soil microbial respiration monitoring.
• Quality control of diagnostic reagents, vaccines, and monoclonal antibody formulations under defined thermal stress protocols.

FAQ

What is the maximum load capacity per shelf?
Each adjustable stainless steel shelf supports up to 15 kg uniformly distributed load; total internal volume is 492 L.
Does the unit support humidity control?
No—the LRH-500F is a temperature-only incubator. For humidity-enabled operation, consider the YiHeng HPG series.
Is calibration certificate included with shipment?
A factory-issued calibration report (traceable to NIM, China) is provided; UKAS or DAkkS-accredited certificates are available upon request.
Can the incubator be validated for GMP use?
Yes—full IQ/OQ documentation templates, sensor mapping protocols, and installation qualification checklists are supplied with optional validation support package.
What is the expected service life of the compressor under continuous operation?
Rated for ≥30,000 hours MTBF (mean time between failures) when operated within ambient conditions of 10–30 °C and relative humidity ≤80% non-condensing.