YSI 2500 / 2900 / 2950 Biochemical Analyzers
| Brand | INFORS |
|---|---|
| Origin | Switzerland |
| Manufacturer Type | Authorized Distributor |
| Origin Category | Imported |
| Model | YSI 2500 / 2900 / 2950 |
| Pricing | Available Upon Request |
Overview
The YSI 2500, 2900, and 2950 Biochemical Analyzers are benchtop enzymatic biosensor systems engineered for rapid, selective, and reproducible quantification of key metabolites and ions in complex biological matrices. Based on Clark-type amperometric detection coupled with immobilized enzyme membranes, these analyzers operate on well-established electrochemical principles: substrate-specific enzymatic reactions generate stoichiometric quantities of hydrogen peroxide (H₂O₂), which is oxidized at a platinum working electrode to produce a current proportional to analyte concentration. For ammonium (NH₄⁺) and potassium (K⁺), ion-selective electrodes (ISEs) provide direct potentiometric measurement. This dual-sensor architecture enables simultaneous multi-analyte profiling without chromatographic separation—ideal for real-time bioprocess monitoring where speed, robustness, and minimal sample preparation are critical.
Key Features
- Rapid analysis: Full quantitative results for >10 analytes—including glucose, lactate, glutamine, glutamate, ammonium, potassium, ethanol, methanol, xylose, sucrose, galactose, lactose, choline, glycerol, and hydrogen peroxide—in ≤60 seconds per sample.
- Anti-clogging fluidic architecture: Patented chamber-based flow design prevents occlusion even in high-cell-density suspensions (e.g., CHO or E. coli fermentations up to 10⁸ cells/mL), eliminating frequent line flushing or membrane replacement.
- Modular scalability: The YSI 2950 platform supports expansion from 2 to 6 independent analyte channels via plug-in sensor cartridges, enabling lab-specific assay configuration without hardware overhaul.
- Intuitive HMI: YSI 2900 and 2950 models feature a 7-inch capacitive touchscreen interface with graphical workflow navigation, context-sensitive prompts, and on-screen calibration verification—designed for GLP-compliant operation by non-specialist users.
- Automated system diagnostics: Integrated liquid-level sensors monitor reagent, calibration, and waste reservoirs; real-time electrode health monitoring detects membrane degradation or electrolyte depletion and triggers user alerts prior to data drift.
- Temperature-compensated measurement: On-board thermistor in the sample module continuously measures fluid temperature; all sensor responses are dynamically corrected against calibration-temperature reference curves per ISO 8655-5 guidelines.
Sample Compatibility & Compliance
These analyzers accept undiluted, unfiltered samples ranging from mammalian cell culture supernatants and microbial fermentation broths to clinical serum, plasma, and food-grade extracts. No centrifugation or filtration is required for typical bioprocess applications. All models comply with CE marking requirements under Directive 2014/30/EU (EMC) and 2014/35/EU (LVD), and conform to RoHS 2011/65/EU restrictions on hazardous substances. Data integrity features—including audit-trail-enabled calibration logs, user-access controls, and timestamped result export—support alignment with FDA 21 CFR Part 11 and EU Annex 11 expectations for regulated environments.
Software & Data Management
YSI BioView™ software (v5.x) provides full instrument control, method storage, and raw-data visualization across Windows-based workstations. It supports automated batch processing for up to 96 samples using standard microtiter formats (single wells, 8-tube strips, 24-well, and 96-well plates). All measurements are stored with metadata including operator ID, calibration history, sensor lot numbers, ambient temperature/humidity, and real-time QC flagging. Export options include CSV, Excel (.xlsx), and PDF report generation compliant with ISO/IEC 17025 documentation standards. Optional LIMS integration via ASTM E1384-compliant HL7 messaging is available for enterprise-scale deployment.
Applications
- Biopharmaceutical process development and manufacturing: Real-time monitoring of glucose, lactate, and glutamine during fed-batch and perfusion cultures to support PAT (Process Analytical Technology) initiatives.
- Fermentation optimization: Quantitative tracking of ethanol, methanol, and ammonium in biofuel production (e.g., yeast-based ethanol or Pseudomonas-derived rhamnolipids).
- Clinical research laboratories: Rapid assessment of metabolic markers in human serum/plasma for diabetes, sepsis, or inborn errors of metabolism studies.
- Food & beverage QA/QC: Batch-wise verification of sugar profiles (sucrose, lactose, fructose), alcohol content, and spoilage indicators (e.g., H₂O₂ in dairy products).
- Academic and industrial biotechnology: High-throughput screening of engineered strains using multi-analyte metabolic phenotyping.
FAQ
What is the minimum sample volume required for analysis?
10–50 µL, user-definable per assay protocol.
Do the YSI 2900 and 2950 models support remote operation or network connectivity?
Yes—Ethernet and USB interfaces enable remote control via BioView™ software; optional Wi-Fi module available for cloud-synced calibration updates.
How often must enzyme membranes be replaced?
Typical lifetime is 3–6 months under continuous bioreactor sampling (24/7); shelf life is 12 months when refrigerated at 4°C.
Is method validation documentation provided for regulatory submissions?
Yes—YSI supplies IQ/OQ protocols, system suitability test templates, and analytical method transfer packages aligned with ICH Q2(R2) guidance.
Can the YSI 2500 perform multi-analyte measurements?
No—the YSI 2500 is a single-channel analyzer; multi-analyte capability begins with the YSI 2900 (dual-channel) and expands fully in the YSI 2950 (2–6 channels).
