PerkinElmer Lambda EZ150 UV-Vis Spectrophotometer (Refurbished)

Overview

The PerkinElmer Lambda EZ150 UV-Vis Spectrophotometer is a compact, high-performance double-beam spectrophotometer engineered for routine quantitative and qualitative analysis in academic, pharmaceutical, and industrial quality control laboratories. Operating on the principle of absorption spectroscopy across the 190–1100 nm wavelength range, the instrument utilizes a holographic grating monochromator and a low-noise silicon photodiode detector to deliver stable baseline performance and high photometric reproducibility. Designed with robust optical alignment and thermally stabilized electronics, the Lambda EZ150 maintains consistent spectral resolution (≤1.8 nm) and wavelength accuracy (±0.5 nm) under standard laboratory conditions. This refurbished unit has undergone full functional validation—including wavelength calibration verification against NIST-traceable holmium oxide and didymium filters, photometric linearity assessment per ASTM E275, and stray light evaluation at 220 nm and 340 nm—prior to release from SpectraLab Scientific’s certified refurbishment facility.

Key Features

• Double-beam optical design with real-time reference compensation for enhanced stability during extended measurements
• Wavelength scanning capability from 190 nm to 1100 nm with programmable scan speed (up to 2400 nm/min) and data point intervals as fine as 0.1 nm
• Integrated 6-position sample holder accommodating 10 mm pathlength cuvettes (standard), with optional accessories for microvolume, flow cell, or solid-sample reflectance measurements
• Onboard data processing including absorbance, transmittance, and concentration modes; kinetic and multi-wavelength analysis supported via embedded firmware
• USB interface compliant with Windows-based host systems; no proprietary drivers required for basic operation and data export
• Refurbished unit includes recalibrated lamp assembly (deuterium and tungsten-halogen), cleaned optics, verified slit function, and updated firmware (v3.2.1)

Sample Compatibility & Compliance

The Lambda EZ150 supports standard quartz, glass, and plastic cuvettes for liquid-phase analysis of aqueous, organic, and buffered solutions. Its optical path geometry accommodates standard 10 mm square cells and is compatible with commercially available temperature-controlled holders (e.g., Peltier-based units up to ±0.1 °C stability). The system conforms to key international standards governing spectrophotometric performance: ASTM E275 (Standard Test Methods for Description and Measurement of Performance of UV-Vis Spectrophotometers), ISO 6233 (Spectrophotometers — Specification and Verification), and USP for absorbance uniformity testing. While not pre-configured for 21 CFR Part 11 compliance, the instrument’s audit-ready data export (CSV/TXT) and timestamped measurement logs support integration into GLP/GMP workflows when paired with validated laboratory information management systems (LIMS).

Software & Data Management

This refurbished Lambda EZ150 operates natively using PerkinElmer’s EZTest software—a lightweight, Windows-compatible application enabling method creation, spectral acquisition, quantitative calibration (single/multi-point), and report generation. All data files are stored in plain-text CSV format with embedded metadata (wavelength, absorbance, date/time stamp, operator ID field), ensuring long-term readability and interoperability with third-party analysis tools such as MATLAB, Python (pandas), or Excel. No cloud dependency or subscription licensing is required. SpectraLab Scientific provides a complete software installation package, including digital license activation and configuration documentation. Raw spectral data retains full fidelity without compression or proprietary binary encoding.

Applications

• Quantitative determination of nucleic acids and proteins using A260/A280 ratios and extinction coefficient-based calculations
• Pharmaceutical assay development and dissolution testing per USP and
• Colorimetric enzyme kinetics (e.g., LDH, ALP, G6PD assays) with time-resolved absorbance monitoring
• Water quality analysis including nitrate, phosphate, and heavy metal complex quantification via standard colorimetric methods
• Educational use in undergraduate analytical chemistry laboratories for Beer-Lambert law validation and spectral identification exercises
• Raw material identity testing and purity assessment in chemical manufacturing QA/QC environments

FAQ

Is this instrument covered by factory warranty or original manufacturer support?
No. As a professionally refurbished unit, it carries a 6-month limited hardware warranty administered directly by SpectraLab Scientific. PerkinElmer technical support is not included but may be engaged separately under paid service contracts.
Does the system include a computer or dedicated acquisition workstation?
No. The Lambda EZ150 requires an external Windows PC (Windows 7 or later, USB 2.0 port) for software operation. A compatible host system is not supplied but can be recommended upon request.
Can I perform GMP-compliant measurements with this unit?
Yes—provided your lab implements appropriate procedural controls. The instrument supports traceable calibration, electronic records with timestamps, and exportable raw data. Full 21 CFR Part 11 compliance requires supplemental LIMS integration and user access controls, which are outside the scope of the base hardware.
What documentation accompanies the shipment?
Each unit ships with a Certificate of Refurbishment, test report summarizing wavelength accuracy, photometric linearity, and stray light results, quick-start guide, and software installation media. Full OEM manuals are available digitally upon request.
Are spare lamps and cuvettes available for purchase?
Yes. SpectraLab Scientific maintains inventory of genuine PerkinElmer deuterium and tungsten-halogen lamps, as well as matched quartz cuvette sets (10 mm pathlength, 3.5 mL volume), all supplied with individual certification of transmittance homogeneity.