Varian Cary 300 UV-Vis Spectrophotometer (Refurbished)
| Brand | Varian |
|---|---|
| Origin | USA |
| Model | Cary 300 |
| Product Category | Spectroscopy |
| Instrument Age | 3–4 years |
| Warranty | 6 months |
| Configuration | Complete system, fully tested and calibrated |
| Compliance | Meets original Varian factory specifications for optical performance and photometric accuracy |
Overview
The Varian Cary 300 UV-Vis Spectrophotometer is a high-performance, double-beam scanning spectrophotometer engineered for precision quantitative and qualitative analysis across the ultraviolet and visible spectral range (190–1100 nm). Based on a Czerny-Turner monochromator design with a high-efficiency holographic grating and thermoelectrically cooled photomultiplier tube (PMT) detector, the Cary 300 delivers exceptional photometric accuracy (±0.002 A), low stray light (<0.0001% at 220 nm), and wavelength reproducibility of ±0.08 nm. This refurbished unit has undergone full functional validation—including baseline stability, photometric linearity, wavelength calibration verification, and lamp intensity profiling—per ASTM E275 and ISO 6223 standards. It is suitable for routine QC/QA in pharmaceutical, environmental, and academic laboratories where regulatory traceability and instrument reliability are critical.
Key Features
- Double-beam optical architecture with real-time reference beam correction for enhanced stability against source drift and environmental fluctuations
- High-intensity xenon flash lamp (no warm-up required) and deuterium/tungsten dual-source configuration for extended lifetime and broad spectral coverage
- Variable slit width (0.2–5.0 nm) enabling optimization of resolution versus signal-to-noise ratio for diverse sample types
- Integrated 6-position autosampler compatibility (optional, not included) for unattended multi-sample analysis
- Robust mechanical design with precision-ground optical mounts and sealed monochromator housing to minimize dust contamination and thermal drift
- Refurbishment includes replacement of consumables (lamp, desiccant, cuvette holder gaskets), full optical alignment, and firmware update to latest stable release (v6.3)
Sample Compatibility & Compliance
The Cary 300 supports standard 10-mm pathlength quartz, glass, or plastic cuvettes, as well as microvolume accessories (e.g., 2-µL capillary cells or 5-µL high-pathlength microcells). It accommodates solid samples via optional integrating sphere (Cary 300 Scan Accessory Kit) and thin-film measurements using variable-angle reflectance attachments. All refurbished units are verified to comply with USP , EP 2.2.25, and ISO/IEC 17025 requirements for analytical instrument qualification. Full IQ/OQ documentation packages—including installation checklist, operational test protocols, and calibration certificates traceable to NIST SRMs—are available upon request for GxP-regulated environments.
Software & Data Management
Controlled by Cary WinUV v6.x software (Windows 10/11 compatible), the system provides comprehensive data acquisition, processing, and reporting tools—including kinetic, scanning, quantitation, and multi-component analysis modules. Audit trail functionality complies with FDA 21 CFR Part 11 when configured with user access controls, electronic signatures, and secure database logging. Raw absorbance, transmittance, and derivative spectra are exportable in ASCII, CSV, or JCAMP-DX formats for integration into LIMS or third-party chemometrics platforms. Software license is perpetual and transferable; no annual subscription fees apply.
Applications
- Pharmaceutical assay development and dissolution testing per USP
- Protein quantification (Bradford, Lowry, BCA) and nucleic acid purity assessment (A260/A280 ratios)
- Environmental water quality monitoring (nitrate, phosphate, COD, heavy metal chelates)
- Chemical kinetics studies with time-resolved scanning up to 24,000 nm/min
- Educational laboratory instruction in physical chemistry and analytical spectroscopy
- Quality control of dyes, pigments, and optical filters in manufacturing settings
FAQ
Is this unit covered by original Varian service support?
No—Varian was acquired by Agilent Technologies in 2010, and official factory support for Cary 300 ended in 2018. However, this unit is backed by our 6-month hardware warranty and technical assistance from certified spectroscopy engineers.
Does the refurbishment include wavelength calibration certification?
Yes—each unit ships with a NIST-traceable wavelength calibration certificate generated using holmium oxide and didymium glass standards per ASTM E275 Annex A1.
Can I add a Peltier temperature-controlled cuvette holder post-purchase?
Yes—the Cary 300 chassis retains full mechanical and electrical compatibility with Varian’s original Cary Temperature Control Unit (TCU), which can be sourced separately and integrated onsite.
What documentation is provided with shipment?
You receive a complete refurbishment report, functional test summary, calibration certificate, safety inspection record, and WinUV software installation media with activation key.
Is remote diagnostics supported?
Yes—WinUV includes built-in diagnostic utilities, and our support team can perform secure remote troubleshooting via TeamViewer (with customer consent) during warranty period.
