Supelco Visiprep DL Solid Phase Extraction Manifold (SPE-12 / SPE-24)

Overview

The Supelco Visiprep DL Solid Phase Extraction Manifold (SPE-12 / SPE-24) is a manually operated, vacuum-driven sample preparation platform engineered for high-reproducibility solid-phase extraction of liquid samples prior to chromatographic or spectroscopic analysis. Based on the fundamental principle of selective adsorption and elution—where analytes are retained on sorbent-packed cartridges while interferences are washed away—the system enables consistent, scalable cleanup of complex matrices including environmental water, biological fluids, food extracts, and pharmaceutical formulations. Designed and manufactured in the United States, this manifold adheres to laboratory practices aligned with ISO/IEC 17025 method validation requirements and supports GLP-compliant workflows when integrated with documented SOPs and audit-trail-capable data recording systems.

Key Features

• Anti-cross-contamination architecture: Independent flow paths and isolated vacuum channels eliminate carryover between adjacent ports—critical for trace-level quantitation and regulatory submissions.
• Precision spiral-flow control valves: Each port features a chemically resistant PTFE-sealed valve enabling reproducible, operator-independent flow rate adjustment across all 12 or 24 positions simultaneously.
• Chemically inert wetted components: Borosilicate glass reservoir chamber resists solvent-induced clouding, cracking, or leaching; PTFE-lined stainless steel solvent delivery needles ensure compatibility with aggressive solvents (e.g., DCM, THF, acetonitrile).
• Ergonomic design: Lid-mounted support feet allow stable placement on benchtops during cartridge exchange; vacuum gauge and integrated pressure-release valve provide real-time monitoring and controlled venting.
• Modular collection system: Interchangeable polypropylene collector racks accommodate standard labware—including 1–10 mL volumetric flasks, 10 mm and 16 mm test tubes, scintillation vials, and autosampler vials—enabling seamless downstream integration.

Sample Compatibility & Compliance

The Visiprep DL manifold accommodates all commercially available 1 mL, 3 mL, 6 mL, 12 mL, 20 mL, and 60 mL SPE cartridges—including Supelco’s own ENVI™, LC–18, and Strata™ series—as well as third-party columns meeting standard threading and dimensional specifications. Its construction complies with USP for plastic components and ASTM D5291 for solvent resistance testing. When used with certified reference materials and validated protocols, the system meets method requirements outlined in EPA Methods 508.1, 525.3, and 8270D, as well as ISO 17993:2022 for environmental sample preparation. The absence of metal-to-solvent contact in critical fluid pathways minimizes trace metal contamination risks essential for ICP-MS and AAS applications.

Software & Data Management

As a manual vacuum manifold, the Visiprep DL operates without embedded firmware or digital controls. However, it is fully compatible with laboratory information management systems (LIMS) and electronic lab notebooks (ELN) through standardized procedural documentation. Users may integrate time-stamped video logging, barcode-scanned cartridge lot tracking, and digital SOP execution via external platforms. For regulated environments, the system supports 21 CFR Part 11 compliance when paired with validated electronic records—e.g., recording valve settings, vacuum pressure logs, and sample batch identifiers in secure, audit-trail-enabled software. Optional accessories—including the 1000 mL polypropylene buffer flask (Cat. No. 51153) and oil-free diaphragm vacuum pump (Cat. No. 501-2001)—are certified for Class II biosafety cabinet use and meet UL/CSA electrical safety standards.

Applications

• Environmental analysis: Pre-concentration and cleanup of pesticides, PAHs, PCBs, and endocrine disruptors from drinking water, wastewater, and soil leachates.
• Clinical toxicology: Isolation of drugs of abuse, metabolites, and biomarkers from plasma, urine, and oral fluid—supporting LC–MS/MS workflows requiring low ng/L detection limits.
• Food safety: Removal of lipids, pigments, and co-extractives prior to mycotoxin or veterinary drug residue analysis per EU Commission Regulation (EC) No 401/2006.
• Pharmaceutical QC: Desalting and buffer exchange of peptide digests or small-molecule APIs prior to HPLC or UPLC separation.
• Forensic chemistry: High-throughput sample prep for seized drug analysis, complying with SWGDRUG Category C validation guidelines.

FAQ

What vacuum source is required for optimal performance?
A certified oil-free diaphragm vacuum pump delivering ≥25 L/min at ≤100 mbar is recommended to maintain stable negative pressure across all ports during sequential elution steps.
Can the manifold handle viscous or particulate-laden samples?
Yes—when used with large-volume samplers (e.g., Cat. No. 51719 or 51688), the system supports direct loading of clarified samples up to 1 L; filtration via inline syringe filters (0.45 µm PTFE) is advised for turbid matrices.
Is sterilization or autoclaving supported?
The glass cylinder and PTFE components are autoclavable at 121°C for 20 minutes; polypropylene collector racks and valves require cold chemical disinfection only.
How is cross-contamination prevented during multi-sample runs?
Each port operates under independent vacuum control via dedicated spiral valves, and PTFE transfer tubing (Cat. No. 51598) is replaced between cartridges—eliminating shared fluid pathways and ensuring analytical integrity per ISO 5725-2 precision criteria.
Are replacement parts traceable and lot-controlled?
Yes—Supelco supplies serialized valves, needles, and tubing with CoA documentation; all consumables carry manufacturer-assigned lot numbers for full chain-of-custody reporting in regulated laboratories.