Tecan Freedom EVOlyzer® Fully Automated ELISA Workstation
| Brand | Tecan |
|---|---|
| Origin | Switzerland |
| Model Variants | EVOlyzer 100, EVOlyzer 150, EVOlyzer 200 |
| Deck Positions | 20 / 28 / 44 |
| Liquid Handling Range | 10–100 µL |
| Pipetting Accuracy | ±10% at 10 µL, ±2% at 100 µL, ±1.5% at 200 µL, ±1% at 1000 µL |
| Sample Throughput (per run) | 2–8 microplates |
| Dimensions (W×D×H) | 800 × 1520 × 910 mm |
Overview
The Tecan Freedom EVOlyzer® Fully Automated ELISA Workstation is an integrated, benchtop liquid handling platform engineered specifically for end-to-end automation of enzyme-linked immunosorbent assay (ELISA) workflows. Based on Tecan’s proven EVO® robotic architecture, the EVOlyzer combines precision liquid handling, thermal incubation, plate washing, and optical detection in a single, modular system. It operates on the principle of coordinated robotic arm movement, multi-channel pipetting, and programmable deck configuration to execute standardized immunoassay protocols—including sample dispensing, serial dilution, reagent addition, controlled incubation (with optional temperature regulation), aspiration/wash cycles using dual- or multi-tip wash heads, and endpoint absorbance/fluorescence reading. Designed for clinical diagnostics, research immunology, and quality control laboratories, the EVOlyzer supports reproducible, audit-ready ELISA execution under GLP and GMP-aligned operational frameworks.
Key Features
- Modular deck configurations supporting 20, 28, or 44 position layouts—enabling flexible integration of deep-well plates, reagent reservoirs, tip racks, incubators, wash modules, and readers.
- High-precision air-displacement pipetting with calibrated accuracy across four volume ranges (10, 100, 200, and 1000 µL), validated per ISO 8655-2 and ASTM E2758 standards.
- Integrated incubation capability with adjustable temperature control (ambient to 45 °C) and condensation management for consistent assay kinetics.
- Dual-mode washing system: configurable for 96- or 384-well microplates with programmable soak times, aspiration speed, and residual volume minimization.
- On-deck LED status indicators provide real-time visual feedback for consumable placement, tip loading, and reagent availability—reducing operator dependency and misloading errors.
- Acoustic and visual alerts activate during critical intervention points (e.g., tip exhaustion, plate misalignment, or low reagent volume), ensuring protocol continuity and operator awareness.
Sample Compatibility & Compliance
The EVOlyzer accommodates standard ANSI/SLAS-format microplates (96- and 384-well), tube racks (including 1.5 mL and 15 mL conical tubes), and custom labware via user-defined deck mapping. It supports common ELISA substrates (TMB, OPD, ABTS), conjugated antibodies, blocking buffers, and wash solutions (PBS, Tris-buffered saline). The system complies with IEC 61000-6-2 (EMC immunity) and IEC 61000-6-3 (EMC emission) standards. Its software architecture supports 21 CFR Part 11 compliance when deployed with Tecan’s iSW software suite—including electronic signatures, audit trails, and role-based access control. All hardware components meet RoHS and CE directives; validation documentation (IQ/OQ/PQ templates) is available for laboratory qualification.
Software & Data Management
Controlled by Tecan’s Run Control software—designed for intuitive touch-screen interaction—the EVOlyzer enables rapid method development without scripting expertise. Protocols are built using drag-and-drop workflow editors with embedded parameter validation (e.g., volume limits, collision avoidance, liquid class selection). The software logs all instrument events, including pipette calibration timestamps, deck initialization sequences, and error recovery actions. Raw absorbance/fluorescence data export adheres to ASTM E1990-compliant CSV and XML formats. Integration with LIS/HIS systems is supported via HL7 v2.x and ASTM LRS interfaces. Data integrity safeguards include automatic backup to network drives, encrypted local storage, and time-stamped revision history for every protocol modification.
Applications
The EVOlyzer is routinely deployed in clinical virology labs for serological testing (e.g., anti-HIV, HBsAg, SARS-CoV-2 IgG/IgM); in biopharmaceutical QC for potency assays and host-cell protein quantification; and in academic immunology for cytokine profiling (IL-6, TNF-α, IFN-γ) across large cohort studies. Its flexibility allows adaptation to competitive, sandwich, indirect, and capture ELISA formats—plus hybrid workflows combining ELISA with downstream qPCR sample prep or mass spectrometry sample cleanup. High-reproducibility performance has been demonstrated in inter-laboratory studies published in Clinical Chemistry and Journal of Immunological MethodsFAQ
Can the EVOlyzer perform serial dilutions automatically?
Yes—using its multi-channel pipettor and dynamic liquid class definition, it executes precise, traceable serial dilutions directly on-deck with minimal manual input.
Is remote monitoring supported?
Yes—via Tecan’s CloudLink module, users can receive push notifications for run completion, error conditions, or maintenance reminders through web or mobile interfaces.
Does it support third-party plate readers?
The EVOlyzer is compatible with select external readers (e.g., Tecan Infinite®, BMG CLARIOstar®) via standardized plate transfer routines and trigger synchronization protocols.
What validation support is provided for regulated environments?
Tecan supplies comprehensive validation packages—including risk assessments, URS templates, FAT/SAT reports, and IQ/OQ/PQ test scripts—aligned with ISO 13485 and FDA guidance for automated clinical instrumentation.
How often does the system require recalibration?
Pipetting performance verification is recommended every 3 months or after 5000 pipetting cycles; full calibration is performed using gravimetric methods traceable to NIST standards.
