Junyi JY-CX1B Isoelectric Focusing Electrophoresis Tank

Overview

The Junyi JY-CX1B Isoelectric Focusing Electrophoresis Tank is a purpose-engineered platform for high-resolution isoelectric focusing (IEF) of proteins and peptides in polyacrylamide or agarose gels. Unlike conventional slab-gel electrophoresis systems, the JY-CX1B employs a passive thermal management architecture based on direct conductive coupling between the gel cassette and a precision-machined, anodized aluminum heat-dissipating plate. This design eliminates the need for active water cooling while maintaining uniform temperature distribution across the entire gel surface—critical for minimizing band distortion, streaking, and pH gradient instability during IEF runs. The system supports standard IEF gel formats with dimensions of 180 mm (width) × 420 mm (length) and a fixed gel thickness of 0.4 mm, enabling reproducible separation of analytes with pI differences as low as 0.01 units under optimized conditions. Its modular electrode assembly and dual-chamber buffer configuration (upper chamber with drain port, lower chamber detachable) facilitate rapid buffer exchange and reduce cross-contamination risk between runs.

Key Features

• Passive thermal regulation via ultra-flat, anodized aluminum散热 plate—ensures even heat dissipation across full gel surface without external coolant circulation
• Four-point mechanical clamping system—enables rapid, uniform gel sandwich assembly with minimal operator training
• Removable electrode holder—simplifies cleaning, inspection, and replacement of platinum or stainless-steel electrodes without disassembling the main tank body
• Integrated shark-tooth comb (red-tinted acrylic)—enhances visual contrast during sample loading and reduces well-to-well diffusion due to optimized tooth geometry and material wettability
• Lid-activated safety interlock—automatically interrupts power supply upon lid opening, complying with IEC 61010-1 Class I electrical safety requirements for laboratory equipment
• Modular buffer reservoir design—lower chamber detaches cleanly for complete buffer removal; upper chamber includes calibrated drain valve for controlled fluid evacuation

Sample Compatibility & Compliance

The JY-CX1B accommodates standard IEF gels cast in glass plates (200 × 450 mm outer frame, 180 × 420 mm active gel area), compatible with both immobilized pH gradient (IPG) strips and carrier ampholyte-based gels. It supports common IEF-compatible sample buffers (e.g., urea, thiourea, CHAPS, DTT) and is routinely deployed in workflows aligned with ISO/IEC 17025–accredited laboratories performing protein characterization. While not certified to FDA 21 CFR Part 11, its mechanical design and operational repeatability support audit-ready documentation when integrated into GLP-compliant SOPs for biomarker discovery, post-translational modification analysis, and comparative proteomics.

Software & Data Management

The JY-CX1B is a standalone electrophoresis tank with no embedded microcontroller or digital interface. All operational parameters—including voltage ramping, run duration, and current monitoring—are managed externally via a compatible high-voltage power supply (e.g., Junyi JY-300E or equivalent). Users are advised to log run conditions manually or via third-party data acquisition systems that comply with ALCOA+ principles (Attributable, Legible, Contemporaneous, Original, Accurate, Complete, Consistent, Enduring, Available). For traceability, batch records should include gel lot numbers, ampholyte concentration, equilibration time, and ambient lab temperature/humidity at initiation.

Applications

• AFLP (Amplified Fragment Length Polymorphism) profiling for plant and microbial strain differentiation
• Microsatellite (SSR) allele resolution in population genetics studies
• SSCP (Single-Strand Conformation Polymorphism) analysis for mutation screening in clinical and research settings
• Heteroduplex DNA detection in mismatch repair assays and CRISPR editing validation
• DNA footprinting experiments assessing protein–DNA binding sites
• RNase protection assays quantifying transcript abundance and splice variants
• S1 nuclease mapping of RNA 5′/3′ ends and intron–exon boundaries
• Primer extension analysis for transcription start site mapping and promoter activity assessment

FAQ

Does the JY-CX1B include a built-in power supply?
No. It is a passive electrophoresis tank requiring an external high-voltage DC power supply capable of delivering up to 3,000 V and 50 mA (recommended minimum specification).
Can it be used for SDS-PAGE or native PAGE?
Not recommended. Its thermal and chamber geometry are optimized specifically for IEF; use of non-IEF protocols may result in uneven heating or buffer depletion.
What maintenance is required for the aluminum heat-dissipating plate?
Wipe clean with deionized water and lint-free cloth after each run; avoid abrasive cleaners or organic solvents that may degrade the anodized surface.
Is the red shark-tooth comb autoclavable?
No. The comb is made from UV-stable acrylic and is not rated for steam sterilization; clean with 70% ethanol and air-dry.
How is buffer volume verified during setup?
Fill the lower chamber to the engraved “MAX” line (~500 mL); overfilling may cause leakage at the gel seal interface during voltage application.