Junyi JY-SPBT Horizontal Electrophoresis Tank
| Brand | Junyi |
|---|---|
| Origin | Beijing, China |
| Manufacturer Type | Authorized Distributor |
| Product Category | Domestic |
| Model | JY-SPBT |
| Instrument Type | Nucleic Acid Electrophoresis System |
| Primary Component | Horizontal Electrophoresis Tank |
| Gel Dimensions (W×L) | 78 × 100 mm |
| Sample Capacity | 30 wells (10- or 15-well combs, available in 1.0 mm and 2.0 mm thicknesses) |
| Buffer Volume | ~400 mL |
| External Dimensions (L×W×H) | 365 × 105 × 55 mm |
| Net Weight | 0.6 kg |
| Safety Features | Lid-integrated safety interlock, ergonomic tray handle, color-coded loading background, removable electrode assembly, dedicated gel casting comb set |
| Material | High-transparency imported polycarbonate (PC), one-piece injection-molded construction |
Overview
The Junyi JY-SPBT Horizontal Electrophoresis Tank is a compact, CE-compliant benchtop system engineered for routine nucleic acid separation and molecular weight estimation in academic, clinical, and quality control laboratories. Designed according to standard horizontal electrophoresis principles—where DNA fragments migrate through an agarose or polyacrylamide matrix under a uniform DC electric field—the JY-SPBT supports standardized protocols aligned with ISO/IEC 17025–accredited workflows. Its fixed gel bed geometry (78 × 100 mm) ensures consistent field homogeneity across the separation zone, minimizing edge effects and supporting reproducible band resolution. The unit operates as a passive component within a complete electrophoresis setup, requiring external power supply (not included) compliant with IEC 61010-1 safety standards for laboratory electrical equipment.
Key Features
- One-piece injection-molded body fabricated from high-purity, UV-transparent polycarbonate—resistant to common electrophoresis buffers (TAE, TBE, SB) and compatible with ethidium bromide, SYBR® Safe, and other nucleic acid stains.
- Integrated lid safety interlock: Automatically interrupts circuit continuity upon lid opening, meeting EN 61010-1 Class II requirements for operator protection during voltage application.
- Ergonomic tray design with integrated handle and color-contrasted loading surface—enables precise pipetting under ambient lab lighting without auxiliary visual aids.
- Modular electrode assembly: Platinum-coated stainless-steel electrodes mounted on detachable, corrosion-resistant carriers—facilitating cleaning, calibration verification, and replacement without disassembly of the main tank.
- Universal comb compatibility: Accepts standard 10- and 15-well combs in both 1.0 mm and 2.0 mm thicknesses; includes dedicated gel casting frame for consistent well depth and alignment.
- Low-profile footprint (365 × 105 × 55 mm) and lightweight construction (0.6 kg) support space-constrained environments, including biosafety cabinets and mobile diagnostic units.
Sample Compatibility & Compliance
The JY-SPBT accommodates agarose gels (0.7–3.0% w/v) and native or denaturing polyacrylamide gels (4–20% T) for separation of DNA fragments ranging from 50 bp to >20 kbp. It is routinely validated for use with ladder standards (e.g., GeneRuler™ 100 bp DNA Ladder, Lambda HindIII digest) per ASTM D7929-15 guidelines for nucleic acid electrophoretic analysis. The tank meets material biocompatibility requirements per ISO 10993-5 and exhibits no detectable leachables in buffer solutions after 72 h immersion (tested per USP ). While not intrinsically certified for GLP or GMP environments, its design supports audit-ready documentation when paired with calibrated power supplies and traceable staining/imaging systems.
Software & Data Management
As a hardware-only electrophoresis platform, the JY-SPBT does not incorporate embedded firmware or digital interfaces. All operational parameters—including applied voltage (typically 50–150 V DC), run duration, and buffer temperature—are controlled externally via programmable power supplies (e.g., Bio-Rad PowerPac Basic, Thermo Fisher E-Gel® Power Supply). Image acquisition and band quantification are performed post-run using third-party gel documentation systems (e.g., Azure Biosystems cSeries, UVP BioSpectrum) compliant with FDA 21 CFR Part 11 when configured with electronic signatures and audit trail modules. Lab information management systems (LIMS) may log instrument metadata (model, serial number, maintenance date) manually or via barcode scanning of the engraved ID plate.
Applications
- Routine genotyping and PCR product verification in molecular biology teaching labs and core facilities.
- Quality assessment of plasmid preps, restriction digests, and CRISPR editing outcomes prior to sequencing.
- Molecular weight estimation of linear dsDNA using logarithmic calibration curves generated from reference ladders.
- Stability testing of nucleic acid formulations under accelerated storage conditions (e.g., thermal degradation studies).
- Supporting ISO/IEC 17025 method validation for in-house electrophoretic assays in accredited testing laboratories.
FAQ
Does the JY-SPBT include a power supply?
No. It is a passive electrophoresis tank requiring an external DC power source rated for constant voltage or constant current output.
Is the tank compatible with ethidium bromide and SYBR® Gold staining?
Yes—its polycarbonate construction is chemically inert toward common nucleic acid dyes and de-staining solutions.
Can I use custom combs with non-standard well geometries?
Only combs conforming to standard 78 mm width and 1.0/2.0 mm thickness specifications are mechanically and electrically compatible.
What buffer volume is required for optimal performance?
A minimum of 350 mL and maximum of 450 mL of running buffer is recommended to ensure full submersion of the gel and stable thermal dissipation.
Is technical support available for protocol optimization?
Junyi’s authorized distributors provide application notes and troubleshooting guides aligned with common electrophoresis standards (e.g., CLSI MM19-A3, ISO 20387).
