Agilent 1200 Series HPLC System (Refurbished, G1365D MWD Configuration)
| Brand | Agilent Technologies |
|---|---|
| Origin | Canada |
| Manufacturer Type | Authorized Distributor |
| Origin Category | Imported |
| Model | 1200 Series, G1365D |
| Product Category | Chromatography |
| Operational Age | 3–4 years |
| Warranty | 6 months |
| Included Modules | G1379B Degasser, G2226A Nano Pump, G1367D Autosampler, G1330B Thermostat, G1316B Column Compartment, G1365D Multi-Wavelength Detector |
Overview
The Agilent 1200 Series HPLC System is a high-performance, modular liquid chromatography platform engineered for precision, robustness, and method transfer compatibility across analytical laboratories. This refurbished configuration centers on the G1365D Multi-Wavelength Detector (MWD), enabling simultaneous UV-Vis absorbance measurements at up to eight user-defined wavelengths with 1 nm resolution and low-noise photodiode array detection. The system operates on classical reversed-phase, normal-phase, ion-exchange, and size-exclusion chromatographic principles, delivering reliable retention time reproducibility (<0.002 min RSD), peak area precision (<0.5% RSD), and baseline stability suitable for regulated QC environments. Designed for seamless integration into existing Agilent ChemStation or OpenLab CDS workflows, this instrument retains full hardware-level compliance with ASTM D4897 (HPLC system performance verification), ISO/IEC 17025 traceability requirements, and USP chromatographic system suitability criteria.
Key Features
- Refurbished and functionally validated by SpectraLab Scientific’s certified service engineers using Agilent-recommended diagnostic protocols and NIST-traceable reference standards.
- G1365D Multi-Wavelength Detector with 190–950 nm spectral range, 1 nm optical resolution, and dual-beam optics for enhanced baseline stability and drift compensation.
- G2226A Nano Pump delivering flow rates from 0.001–10 mL/min with pulseless operation, gradient accuracy ±0.2%, and pressure capability up to 600 bar—compatible with sub-2 µm particle columns.
- G1367D Autosampler featuring 100-position vial capacity, 0.1–100 µL injection volume range, and temperature-controlled sample compartment (4–40 °C) to preserve thermolabile analytes.
- G1316B Column Compartment with precise temperature control (5–80 °C, ±0.15 °C stability) and dual-column switching capability for method development flexibility.
- Integrated G1379B Vacuum Degasser (four-channel) ensuring consistent mobile phase composition and minimizing baseline noise during gradient elution.
- Full mechanical and electronic recalibration performed prior to shipment; all modules verified for leak integrity, flow accuracy, dwell volume consistency, and detector linearity.
Sample Compatibility & Compliance
This Agilent 1200 HPLC configuration supports aqueous, organic, and mixed-solvent mobile phases (including TFA, HFBA, and ammonium acetate buffers) and accommodates standard 4.6 mm × 150 mm analytical columns as well as narrow-bore (2.1 mm ID) and microbore formats. It meets fundamental performance criteria outlined in ICH Q2(R2) for analytical method validation—including specificity, linearity, accuracy, precision, and robustness assessment. Instrument qualification documentation (IQ/OQ) templates are provided upon request, aligned with FDA 21 CFR Part 11 data integrity expectations for audit trails, electronic signatures, and secure user access controls when operated under OpenLab CDS.
Software & Data Management
The system is delivered with Agilent ChemStation Rev. B.04.03 or later (license included), supporting full acquisition, processing, reporting, and system suitability evaluation. Raw data files (.D directories) are fully compatible with Agilent OpenLab CDS for migration to modernized, networked laboratory environments. All firmware versions are updated to the latest stable release supported for the 1200 Series architecture. Audit trail functionality, electronic signature enforcement, and user role-based permissions can be enabled per GLP/GMP operational policies. Data export options include CSV, PDF, and AIA/ANDI formats for third-party statistical analysis or LIMS integration.
Applications
- Pharmaceutical quality control: assay quantitation, impurity profiling, and dissolution testing per USP monographs.
- Food and beverage safety: pesticide residue screening, mycotoxin analysis, and vitamin stability studies.
- Environmental monitoring: PAHs, PCBs, and phenolic compounds in water and soil extracts.
- Academic and industrial research: natural product purification tracking, polymer molecular weight distribution (SEC-GPC), and chiral separation method development.
- Bioanalytical support: peptide mapping, oligonucleotide purity assessment, and small-molecule PK/PD biomarker quantification.
FAQ
Is this system eligible for extended warranty or service contracts?
Yes—post-warranty Agilent-certified service plans are available through our global partner network, including preventive maintenance visits, remote diagnostics, and priority parts fulfillment.
Can this refurbished 1200 Series be upgraded to support modern software platforms like OpenLab CDS?
Absolutely—the hardware is fully compatible with OpenLab CDS 2.x and later; license activation and migration assistance are included with purchase.
What documentation accompanies the instrument?
You will receive a full refurbishment report, module-specific calibration certificates, system suitability test results, and a signed statement of conformance to Agilent 1200 Series functional specifications.
Are spare parts and consumables available for long-term support?
Yes—we maintain inventory of critical wear items (seals, rotors, lamps, fuses) and offer technical guidance for routine maintenance procedures per Agilent’s published service manuals.
Does the system include a computer and monitor?
A dedicated Windows-based acquisition workstation (with pre-installed ChemStation or OpenLab CDS) is optionally available upon request; contact our sales team for configuration details.
