HUANKONG JHK-3300 Portable High-Flow Bioaerosol Sampler

Overview

The HUANKONG JHK-3300 Portable High-Flow Bioaerosol Sampler is an engineered solution for quantitative collection of airborne biological particles—including bacteria, viruses, fungal spores, pollen, bacteriophages, dust mites, and other respirable bioaerosols—directly into liquid media. It operates on the principle of cyclonic inertial impaction, where high-velocity air is forced into a spiral path within a dry-wall chamber; centrifugal forces drive particulates toward the chamber wall, where they are efficiently eluted into a small volume of collection fluid (e.g., phosphate-buffered saline or viral transport medium). This method avoids filter clogging, preserves microbial viability better than membrane filtration, and yields highly concentrated samples ideal for downstream molecular (qPCR, RT-LAMP), culture-based, or immunoassay analysis. Designed for field-deployable operation, the JHK-3300 maintains stable volumetric flow across variable ambient conditions and supports standardized sampling protocols required in environmental health, outbreak investigation, biosafety assessment, and indoor air quality auditing.

Key Features

• Cyclonic dry-wall concentration architecture enabling high-efficiency (>90% for particles ≥0.5 µm at 300 L/min) and minimal sample dilution
• Adjustable mass-flow-controlled sampling from 100 to 400 L/min, calibrated per JJF 1826–2020 using traceable electronic flow meters
• Integrated environmental compensation: real-time measurement of pre-sampler temperature and pressure for automatic normalization to standard conditions (25 °C, 101.325 kPa)
• 7.0-inch industrial-grade capacitive touchscreen with wide operating temperature range (–10 °C to 50 °C), supporting both touch and physical button operation
• Dual data export: direct Bluetooth thermal printing of sampling logs and bulk export via USB 2.0 to FAT32-formatted drives
• Modular wetted-path design: rapid field replacement of sampling cylinder and tubing with tool-free disassembly, facilitating decontamination and cross-contamination prevention
• Robust internal memory storing >100,000 timestamped records, including flow rate, duration, environmental parameters, and operator ID
• Programmable scheduling: user-defined start time, duration, and repeat intervals; one-touch initiation with audible/visual confirmation
• Power resilience: built-in capacitor-backed memory ensures full data integrity during unexpected power loss; resumes interrupted sampling automatically upon restoration

Sample Compatibility & Compliance

The JHK-3300 is validated for use with standard aqueous collection media (e.g., 10–30 mL sterile saline, RNAlater, or viral lysis buffers) and accommodates optional particle pre-separation heads for concurrent coarse particulate removal (e.g., PM₁₀ cut-point cyclones or impactor stages). Its performance aligns with national standards governing bioaerosol sampling in China, including GB/T 39990–2021 (technical specifications for particle bioaerosol samplers), GB/T 38517–2020 (general principles for bioaerosol sampling and analysis), and GB/T 18204.3–2013 (microbiological testing methods for public spaces). While not certified to ISO 14698 or EU GMP Annex 1, its design facilitates adherence to GLP-aligned documentation practices—including audit-ready metadata logging (time, location, operator, calibration status)—and supports traceability required under FDA 21 CFR Part 11 when paired with validated software workflows.

Software & Data Management

The embedded firmware implements deterministic real-time sampling control and generates structured CSV log files containing: sampling ID, UTC timestamp, actual flow rate (L/min), cumulative volume (L), ambient temperature (°C), ambient pressure (kPa), normalized volume (L_std), battery level (%), and error flags. No proprietary desktop application is required for data retrieval; exported files are compatible with common laboratory informatics systems (LIMS), statistical packages (R, Python pandas), and regulatory submission templates. Firmware updates are delivered via signed USB firmware images, with version rollback capability and SHA-256 hash verification. All stored records include immutable timestamps synchronized to internal RTC with ±2 s/month drift specification.

Applications

• Field-based surveillance of airborne pathogens in hospitals, laboratories, and biosafety level (BSL)-2/3 facilities
• Indoor air quality assessments in schools, offices, and residential buildings per GB/T 18883–2022
• Monitoring bioaerosol dispersion during HVAC system maintenance or remediation of mold-contaminated ductwork (per GB/T 18204.5–2013)
• Environmental monitoring in cleanrooms and controlled pharmaceutical manufacturing environments (aligned with GB/T 25916.1–2010 principles)
• Rapid response sampling at mass gathering venues (e.g., swimming pools, gyms, bathhouses) during public health investigations
• Validation of air disinfection technologies (UVGI, bipolar ionization) through time-series bioaerosol load quantification

FAQ

What collection fluids are compatible with the JHK-3300?
Sterile physiological saline (0.9% NaCl), PBS, viral transport medium (VTM), and RNAlater are routinely used. Viscosity and surface tension must remain within ISO 8573-1 Class 4 limits to ensure consistent cyclonic separation efficiency.
Can the JHK-3300 be used for non-biological aerosols?
Yes—its inertial collection mechanism is particle-size dependent, not biology-specific. However, validation data and regulatory acceptance apply exclusively to bioaerosol applications per referenced Chinese standards.
Is external calibration required before each sampling campaign?
Per JJF 1826–2020, annual calibration by an accredited metrology lab is recommended; field verification using a primary-standard rotameter or critical orifice is advised prior to high-stakes deployments.
Does the device support remote monitoring or network connectivity?
No Ethernet or Wi-Fi interface is integrated. Remote oversight requires third-party IoT gateways interfacing via RS-232 or USB CDC serial emulation, outside the scope of factory validation.
How is cross-contamination prevented between sequential samples?
The sampling cylinder, inlet nozzle, and liquid conduit are fully removable and autoclavable (121 °C, 20 min). The firmware enforces mandatory “clean cycle” confirmation before initiating new sampling sessions.