HUXI HT-X330 Orbital Shaker with Temperature Control

Overview

The HUXI HT-X330 Orbital Shaker with Temperature Control is an engineered platform for precise, reproducible orbital mixing and incubation of biological and chemical samples under controlled thermal conditions. Designed around a brushless DC motor and microprocessor-based control architecture, it delivers stable rotational motion via a 4 mm circular orbit—optimized for uniform suspension, cell culture maintenance, enzyme kinetics assays, and reagent homogenization. Unlike generic benchtop shakers, the HT-X330 integrates Peltier-based thermoregulation to maintain setpoint temperatures between 5 °C and 40 °C with ±0.5 °C stability (at 25 °C ambient), enabling applications requiring simultaneous agitation and low-to-moderate temperature control—such as bacterial growth studies, hybridization protocols, and solubility testing. Its compact footprint (330 × 370 × 130 mm) and IP21-rated enclosure ensure safe operation in standard laboratory environments compliant with IEC 61010-1 safety requirements.

Key Features

• Brushless DC motor drive ensures speed accuracy ±1 rpm across the full 60–500 rpm range, minimal acoustic noise (<45 dB(A)), and maintenance-free operation exceeding 20,000 hours MTBF.
• Large backlit LCD display simultaneously shows real-time values for speed, temperature, and remaining timer duration—supporting intuitive parameter verification during GLP-compliant workflows.
• Locked keypad mode prevents unintended changes to active settings—a critical safeguard during unattended overnight runs or multi-user lab environments.
• Soft-start acceleration profile eliminates sample splashing and tube displacement during startup, preserving integrity of low-volume cultures and fragile gels.
• Modular platform design accommodates interchangeable trays (e.g., universal clamping plates, microplate holders, flask racks) without tools—enabling rapid reconfiguration between Erlenmeyer flasks (up to 2 L), Petri dishes, centrifuge tubes, and deep-well plates.
• Optional RS232 serial interface supports bidirectional communication with external PCs or LIMS systems, allowing remote parameter configuration, real-time data logging, and audit-trail generation per FDA 21 CFR Part 11 requirements when paired with compliant software.

Sample Compatibility & Compliance

The HT-X330 supports routine use with standard laboratory vessels including glass and plastic conical flasks (50 mL–2 L), polystyrene and polypropylene culture dishes, PCR tubes, and 96-well microplates. Its 7.5 kg max load capacity permits balanced loading of multiple vessels without compromising orbital consistency. The device conforms to ISO/IEC 17025 calibration traceability frameworks when used with NIST-traceable thermometers and tachometers. It meets electromagnetic compatibility standards per EN 61326-1 and electrical safety per EN 61010-1. While not certified for sterile or cleanroom use (ISO 14644-1 Class 5+), its smooth stainless-steel-coated platform and non-porous housing facilitate routine decontamination using 70% ethanol or 0.5% sodium hypochlorite solutions.

Software & Data Management

When equipped with the optional RS232 module, the HT-X330 interfaces with third-party data acquisition platforms—including LabVIEW-compatible drivers and custom Python scripts via pySerial. Logged parameters include timestamped speed, temperature, and elapsed time at user-defined intervals (1 s to 60 min). Export formats include CSV and XML, supporting integration into ELN systems such as LabArchives or Benchling. Audit trails record operator ID, parameter changes, and system events—enabling retrospective review aligned with ALCOA+ principles (Attributable, Legible, Contemporaneous, Original, Accurate, Complete, Consistent, Enduring, Available).

Applications

• Microbial cultivation: E. coli, yeast, and mammalian suspension cultures requiring consistent oxygen transfer and thermal stability.
• Immunological assays: Antigen–antibody binding kinetics, ELISA plate washing steps, and secondary antibody incubations.
• Biochemical processing: Protein dialysis, gel staining/destaining, DNA extraction buffer equilibration, and solid-phase peptide synthesis.
• Environmental and pharmaceutical QC: Dissolution testing of coated tablets, heavy metal leaching studies, and colloidal dispersion homogenization.
• Education and training: Undergraduate life science labs performing standardized growth curve experiments under controlled agitation and temperature conditions.

FAQ

Does the HT-X330 support programmable ramping or multi-step protocols?
No—the HT-X330 executes single-setpoint operation only. For multi-stage temperature/speed profiles, users must implement external sequencing via RS232 commands.
Is the temperature control capability validated per ISO 17025?
Validation is user-responsible; the instrument includes factory calibration certificates traceable to national standards, but formal method validation requires in-house IQ/OQ/PQ documentation.
Can the unit operate continuously for >24 hours?
Yes—designed for unattended operation up to 99 hours 59 minutes; thermal protection circuitry automatically shuts down the heater if internal temperature exceeds safe thresholds.
What is the maximum flask size compatible with the standard platform?
A single 2 L Erlenmeyer flask may be mounted centrally; for optimal balance and orbital fidelity, loads exceeding 3 kg should be distributed symmetrically across the platform.
Is firmware upgrade supported?
Firmware updates are available upon request from HUXI technical support and require RS232 connection and proprietary update utility.