IH-SR Isolated Heart Perfusion System (Murine, Rat, and Guinea Pig)
| Brand | BTX |
|---|---|
| Origin | USA |
| Manufacturer Type | Authorized Distributor |
| Origin Category | Imported |
| Model | IH-SR (Murine, Rat and Guinea Pig) |
| Pricing | Available Upon Request |
Overview
The IH-SR Isolated Heart Perfusion System is a rigorously engineered platform for ex vivo cardiac physiology studies in small laboratory mammals—including mice, rats, and guinea pigs. Designed around the classic Langendorff and Working Heart configurations, the system enables precise, real-time assessment of myocardial contractility, coronary perfusion dynamics, electrophysiological stability, and metabolic responsiveness under controlled, physiologically relevant conditions. Its modular architecture integrates pressure-controlled or flow-controlled perfusion circuits with high-fidelity signal acquisition for mechanical (e.g., left ventricular developed pressure, dP/dtmax, heart rate) and bioelectrical parameters (e.g., ECG, monophasic action potentials). The system operates on the principle of retrograde aortic perfusion (Langendorff) or antegrade aortic + atrial filling (Working Heart), maintaining coronary vascular resistance and ventricular afterload via calibrated compliance chambers and adjustable flow resistors—closely replicating in vivo hemodynamic loading conditions.
Key Features
- Configurable dual-mode operation: Langendorff and Working Heart modes supported via interchangeable cannulation kits and pressure/flow control modules.
- Physiologically matched afterload simulation: Integrated compliance chamber and precision flow resistor enable tunable arterial impedance that mirrors native systemic vascular resistance across murine, rat, and guinea pig cardiovascular scales.
- Modular signal acquisition interface: Compatible with standard physiological amplifiers (e.g., ADInstruments PowerLab, BIOPAC MP160) for synchronized recording of LV pressure, coronary flow, ECG, and temperature.
- Pre-validated, pre-calibrated components: All fluidic pathways, heat-exchange manifolds, and gas-permeable oxygenation modules are factory-tested for sterility, bubble-free operation, and thermal stability (37 °C ± 0.2 °C).
- Expandable architecture: Supports integration with optional add-ons including real-time lactate/pH/blood gas analyzers (e.g., ABL90 Flex), high-speed video-based motion analysis, and programmable electrical stimulators for pacing protocols.
Sample Compatibility & Compliance
The IH-SR system is validated for use with intact, excised hearts from C57BL/6, BALB/c, and FVB mice; Sprague-Dawley and Wistar rats; and Hartley guinea pigs (250–400 g). It complies with core methodological standards referenced in ISO 10993-1 (biocompatibility of perfusion circuit materials), ASTM F2119 (in vitro cardiovascular device testing), and ICH S7B/S7A guidelines for proarrhythmic risk assessment. All wetted surfaces are USP Class VI-certified silicone and medical-grade polycarbonate. The system supports GLP-compliant study execution when paired with FDA 21 CFR Part 11–enabled data acquisition software (e.g., LabChart v8.2+ with audit trail and electronic signature modules).
Software & Data Management
Data acquisition is natively supported through HSE-DAQ family platforms—including BDAS (basic digital acquisition suite), HAEMODYN (hemodynamic parameter derivation), and ISOHEART (isovolumic constraint modeling). These tools compute derived metrics such as cardiac output, stroke volume, coronary vascular resistance, and time-domain ECG interval analysis (PR, QRS, QTc). Raw analog signals are digitized at ≥10 kHz sampling rate with 16-bit resolution. Export formats include MATLAB (.mat), HDF5, and CSV for downstream statistical analysis (e.g., R, Python SciPy, GraphPad Prism). Optional modules support automated beat-by-beat annotation, arrhythmia classification (based on Lambeth Conventions), and pharmacodynamic modeling (e.g., Hill equation fitting for inotropic dose-response curves).
Applications
- Evaluation of positive/negative inotropic agents (e.g., dobutamine, verapamil) and vasodilators (e.g., nitroprusside, adenosine) under constant-flow or constant-pressure perfusion.
- Proarrhythmic screening: Assessment of chronotropic, dromotropic, and arrhythmogenic effects—including early afterdepolarizations (EADs), delayed afterdepolarizations (DADs), and reentrant tachyarrhythmias.
- Refractory period mapping via programmed electrical stimulation (PES) protocols integrated with stimulus isolation units.
- Transgenic and knockout model phenotyping: Functional validation of ion channel mutations (e.g., KCNQ1, SCN5A), mitochondrial defects, or hypertrophic cardiomyopathy phenotypes.
- Ischemia-reperfusion injury modeling with precise control over no-flow duration, reperfusion pressure, and reactive oxygen species scavenging interventions.
FAQ
What species-specific adaptations does the IH-SR include for murine hearts?
The system includes micro-bore cannulae (0.4–0.6 mm ID), low-volume compliance chambers (<500 µL), and high-sensitivity pressure transducers (range: 0–300 mmHg, resolution: 0.1 mmHg) optimized for mouse coronary flow rates of 2–6 mL/min.
Can the IH-SR be used for long-term (>120 min) perfusions?
Yes—when operated with continuous gaseous oxygenation (95% O2/5% CO2), temperature-regulated recirculating Krebs-Henseleit buffer, and antioxidant supplementation (e.g., catalase, allopurinol), stable function has been demonstrated for up to 180 minutes in rat and guinea pig preparations.
Is regulatory documentation available for preclinical submission packages?
BTX provides full DMR (Device Master Record) excerpts, IQ/OQ protocols, and traceable calibration certificates for all critical subsystems—supporting IND-enabling toxicology and safety pharmacology studies per ICH M3(R2) and FDA Guidance for Industry.
