JIAYUAN MB100-2A Microplate Incubator Shaker
| Brand | JIAYUAN |
|---|---|
| Origin | Beijing, China |
| Model | MB100-2A |
| Timer Range | 0–99 h 59 min |
| Oscillation Speed | 300–1200 rpm |
| Orbital Diameter | 3 mm |
| Temperature Range | Ambient +5°C to 70°C |
| Capacity | 2 standard microplates (e.g., 96-well, 384-well, deep-well, cell culture plates) |
| Temperature Uniformity | ≤ ±0.5°C |
| Temperature Stability | ≤ ±0.2°C |
| Heating Accuracy | < ±0.3°C at 37°C |
| Ramp Time | < 25 min (20°C → 70°C) |
| Power Consumption | 150 W |
| Dimensions | 284 × 264 × 157 mm (W × D × H) |
Overview
The JIAYUAN MB100-2A Microplate Incubator Shaker is a compact, precision-engineered platform designed for simultaneous temperature-controlled incubation and orbital shaking of standard microplates used in life science workflows. It operates on a PID-regulated resistive heating system with dual-sided thermal plates—top and bottom—to ensure uniform heat distribution across all wells of 96-well, 384-well, deep-well, and cell culture plates. The unit employs a brushless DC motor driving horizontal orbital motion (3 mm diameter), delivering consistent agitation without mechanical wear or electromagnetic interference. Its design prioritizes thermal homogeneity, speed accuracy, and operational repeatability—critical parameters for enzyme-linked immunosorbent assays (ELISA), cell-based assays, protein binding studies, bacterial growth kinetics, and reagent mixing protocols where temperature-dependent reaction kinetics and suspension stability are essential.
Key Features
- Independent dual-zone heating architecture: top and bottom aluminum heating plates minimize vertical thermal gradients and ensure ≤ ±0.5°C temperature uniformity across the entire plate surface
- PID-controlled thermal regulation with real-time feedback loop, achieving ≤ ±0.2°C temperature stability and < ±0.3°C absolute accuracy at 37°C—validated per ISO/IEC 17025 traceable calibration practices
- Brushless DC motor drive ensures maintenance-free operation, low acoustic emission (<45 dB(A)), and long-term speed fidelity across the full 300–1200 rpm range
- Intuitive touch-sensitive LCD interface displaying setpoints and real-time values for temperature, time, and rpm simultaneously
- Programmable timer with extended range (0–99 h 59 min) and auto-restart function following power interruption—supports unattended overnight or multi-day assays
- Compact footprint (284 × 264 × 157 mm) optimized for biosafety cabinets, cold rooms, and shared core facility benchtops
- Compliant with IEC 61000-6-3 (EMC emission) and IEC 61010-1 (safety requirements for laboratory equipment)
Sample Compatibility & Compliance
The MB100-2A accommodates two standard ANSI/SLAS-format microplates per run—including 96-well ELISA plates, 384-well assay plates, 24-/48-/96-well cell culture plates, and 96-well deep-well storage plates (up to 2.2 mL/well). Plate clamping is achieved via spring-loaded side rails with adjustable retention force, preventing lateral displacement during high-speed oscillation. All internal surfaces contacting plates are constructed from anodized aluminum and food-grade stainless steel—non-reactive, autoclavable (except electronics), and compatible with common lab solvents (e.g., 70% ethanol, isopropanol). The device meets general-purpose laboratory safety standards under IEC 61010-1 Ed.3 and supports GLP-compliant documentation when paired with external audit-trail-capable data loggers.
Software & Data Management
While the MB100-2A operates as a standalone instrument with embedded firmware, its parameter settings (temperature, rpm, duration) are fully recallable and reproducible via front-panel memory presets. For integration into regulated environments (e.g., clinical diagnostics or pharmaceutical QC labs), users may connect optional RS-232 or USB-to-serial adapters to export timestamped operational logs—including start/stop events, thermal deviation alerts, and cycle completion signals—to LIMS or ELN systems. Though not natively 21 CFR Part 11 compliant, the hardware architecture supports third-party validation packages that enable electronic signature, audit trail, and user access control when deployed with validated software layers.
Applications
- ELISA and immunoassay plate washing and incubation steps requiring precise thermal control and gentle agitation
- Cell-based assays involving adherent or suspension cultures under controlled temperature and mixing conditions
- Protein-protein interaction studies, ligand-binding kinetics, and antigen-antibody equilibration
- Bacterial and yeast growth profiling across temperature gradients with continuous orbital mixing
- Sample homogenization prior to downstream analysis (e.g., qPCR, NGS library prep, mass spectrometry)
- Reagent resuspension and dissolution in high-throughput screening workflows
FAQ
What plate formats are supported?
Standard ANSI/SLAS-compliant microplates: 96-well, 384-well, deep-well (V- or U-bottom), and cell culture plates (24-, 48-, and 96-well). Plates must conform to SLAS-1-2019 dimensional tolerances.
Is the unit suitable for CO₂-independent cell culture?
Yes—provided ambient CO₂ levels are sufficient for the cell line; the MB100-2A does not regulate gas composition but maintains stable temperature and agitation critical for short-term incubation (≤72 h).
Can the device be calibrated internally?
No internal calibration routine exists; however, it accepts external NIST-traceable PT100 probe verification at multiple setpoints (25°C, 37°C, 55°C, 70°C) per ASTM E2251-22 guidelines.
Does it support staggered ramping profiles?
No—only single-step temperature programming is available. Multi-stage thermal protocols require external controller integration.
What maintenance is required?
None beyond periodic cleaning of heating plates with lint-free cloth and isopropyl alcohol; the brushless motor requires no lubrication or replacement over its rated service life (>20,000 h).
