Junyi JY-CX2B Horizontal DNA Sequencing Electrophoresis Tank

Overview

The Junyi JY-CX2B is a high-capacity horizontal electrophoresis tank engineered for reproducible nucleic acid separation in sequencing-grade applications. It operates on the principle of constant-voltage or constant-current electrophoresis in submerged agarose or polyacrylamide gels, where charged biomolecules migrate through a porous matrix under an applied electric field. Its design prioritizes thermal stability, operational safety, and compatibility with downstream visualization and blotting workflows. Unlike vertical systems, the JY-CX2B supports large-format gels (up to 330 × 420 mm), enabling high-throughput analysis of complex DNA fragment patterns—critical for genotyping, mutation screening, and comparative sequence analysis.

Key Features

• Optimized thermal management: An anodized, ultra-flat aluminum heat-dissipating plate ensures uniform contact with glass plates, minimizing localized heating and preventing band distortion caused by temperature gradients.
• Reduced buffer consumption: Passive conduction cooling eliminates the need for external water circulation, lowering buffer usage by approximately 50% compared to traditional chilled tanks—reducing both operational cost and environmental footprint.
• Modular electrode architecture: Stainless-steel electrodes are mounted on a fully removable frame, facilitating rapid cleaning, inspection, and replacement without disassembling the main tank body.
• Ergonomic fluid handling: Upper buffer chamber includes a calibrated drain tube; lower chamber detaches cleanly for efficient buffer removal and residue-free rinsing.
• Enhanced sample integrity: Red-colored shark-tooth combs provide high-contrast visual confirmation during loading and reduce cross-well diffusion due to optimized tooth geometry and precise gel interface alignment.
• Integrated safety logic: Lid-mounted microswitch automatically interrupts power supply upon opening—complying with IEC 61010-1 Class II electrical safety requirements for laboratory equipment.

Sample Compatibility & Compliance

The JY-CX2B accommodates standard 0.4 mm thick gels cast in reusable glass plates, supporting agarose concentrations from 0.8% to 2.0% and polyacrylamide gradients from 4% to 20%. It is routinely employed in protocols aligned with ISO/IEC 17025-accredited molecular biology laboratories. While the unit itself does not include integrated power supply, it is compatible with certified DC power supplies meeting EN 61326-1 (EMC) and EN 61010-1 (safety) standards. The tank’s materials—polypropylene housing, borosilicate glass plates, and stainless-steel electrodes—are chemically resistant to common electrophoresis buffers (TAE, TBE, SB) and decontamination agents (e.g., 10% sodium hypochlorite, 70% ethanol).

Software & Data Management

As a standalone electrophoresis tank, the JY-CX2B does not incorporate embedded firmware or digital connectivity. However, its standardized dimensions and consistent gel geometry ensure seamless integration with third-party imaging platforms—including CCD-based gel documentation systems compliant with FDA 21 CFR Part 11 (when paired with validated acquisition software). Users may archive electrophoretic run parameters (voltage, time, buffer lot number) manually within LIMS or ELN environments following GLP/GMP-aligned record-keeping practices. The absence of proprietary controllers simplifies validation and reduces long-term maintenance dependencies.

Applications

• AFLP (Amplified Fragment Length Polymorphism) profiling for plant and microbial strain differentiation
• Microsatellite (SSR) and SNP-linked marker analysis in population genetics studies
• SSCP (Single-Strand Conformation Polymorphism) screening for point mutation detection in clinical diagnostics
• Heteroduplex analysis in mismatch repair assays and CRISPR editing validation
• DNA footprinting experiments assessing protein–DNA binding sites
• RNase protection assays quantifying transcript abundance and splice variants
• S1 nuclease mapping for precise 5′/3′ end determination of RNA species
• Primer extension analysis for transcription start site mapping and allelic expression quantification

FAQ

Is the JY-CX2B compatible with UV transilluminators and gel imagers?
Yes—the transparent polypropylene tank body and standard gel dimensions allow unobstructed placement beneath most commercial UV and blue-light transilluminators, as well as CCD-based documentation stations.
Can I use this tank for denaturing PAGE (e.g., urea gels)?
Yes, provided appropriate gel casting accessories (e.g., spacers, comb inserts) and buffer systems (e.g., 7 M urea in TBE) are used; the tank’s chemical resistance and thermal stability support such applications.
Does the unit include a power supply?
No—the JY-CX2B is a passive electrophoresis tank only; a separate DC power supply rated for ≥500 V and ≥500 mA is required for operation.
What is the recommended buffer volume for optimal performance?
Approximately 750 mL ensures full submersion of the 330 × 420 mm gel while maintaining stable conductivity and minimizing edge effects; overfilling may cause leakage, underfilling risks arcing.
How often should the aluminum heat-dissipating plate be cleaned?
After each use—wipe gently with lint-free cloth and 70% ethanol; avoid abrasive cleaners to preserve the anodized surface integrity and thermal contact efficiency.