Junyi JY-CZ-BL Vertical Electrophoresis Tank

Overview

The Junyi JY-CZ-BL Vertical Electrophoresis Tank is an engineered platform for high-resolution isoelectric focusing (IEF) and standard SDS-PAGE applications in molecular biology and protein biochemistry laboratories. Designed around the principles of vertical slab-gel electrophoresis, it supports separation based on charge-to-mass ratio (IEF) or molecular weight (SDS-PAGE) under controlled electric field conditions. Its robust acrylic construction ensures chemical resistance to common electrophoresis buffers—including Tris-Glycine, Tris-Tricine, and urea-based IEF solutions—while maintaining dimensional stability during extended runs. The system operates within standard laboratory voltage ranges compatible with most commercial power supplies (typically 100–500 V DC), and its integrated safety architecture—including automatic circuit interruption upon lid opening—meets fundamental electrical safety expectations for Class I laboratory equipment.

Key Features

• In-situ gel casting capability: Eliminates post-casting gel transfer, reducing contamination risk and mechanical damage to fragile polyacrylamide gels; accommodates both hand-poured and pre-cast gel formats via interchangeable inner cassettes.
• Modular comb system: Supports 25-, 40-, and 52-well combs (1.0 mm thickness), enabling flexible sample throughput across diverse experimental designs including SSR fragment analysis and protein isoform profiling.
• Optimized thermal management: Large-volume buffer reservoir (~8000 mL) provides stable pH buffering and passive heat dissipation; optional external chiller port enables connection to recirculating temperature control units (e.g., Julabo or Thermo Scientific models) for temperature-sensitive IEF or high-voltage PAGE protocols.
• Ergonomic sample loading aids: Integrated sample guide comb and high-contrast background plate improve pipetting accuracy and reduce misloading errors during multi-lane assays.
• Fail-safe electrical design: Microswitch-actuated power cutoff engages immediately upon lid displacement, complying with general lab safety best practices aligned with IEC 61010-1 requirements for electrical equipment used in laboratory environments.

Sample Compatibility & Compliance

The JY-CZ-BL accommodates standard 186 × 205 mm polyacrylamide gels (1.0 mm thick), suitable for analytical-scale protein separation, nucleic acid fragment analysis (e.g., SSR amplicons), and two-dimensional electrophoresis first-dimension IEF strips. It is compatible with common gel matrices including acrylamide-bisacrylamide mixtures, agarose overlays, and immobilized pH gradient (IPG) strips. While not certified to ISO/IEC 17025 or FDA 21 CFR Part 11 out-of-the-box, its operational consistency supports GLP-aligned documentation workflows when paired with validated power supplies and calibrated pipettes. Users performing regulated testing (e.g., clinical research or QC of recombinant proteins) should implement supplementary instrument qualification (IQ/OQ) per internal SOPs.

Software & Data Management

As a benchtop electrophoresis tank, the JY-CZ-BL operates without embedded firmware or proprietary software. All parameter control—including voltage, current, and run duration—is managed externally via connected power supplies. Data acquisition and image analysis are performed post-electrophoresis using third-party imaging systems (e.g., Bio-Rad ChemiDoc, Azure Biosystems Sapphire) and open-format software (ImageLab, Fiji/ImageJ). This hardware-only architecture ensures full compatibility with institutional IT policies restricting vendor-specific drivers or cloud-linked instrumentation.

Applications

• High-throughput SSR (Simple Sequence Repeat) genotyping via PAGE-based fragment sizing
• Protein isoform resolution using isoelectric focusing in the first dimension of 2D-PAGE
• Verification of recombinant protein purity and post-translational modification profiles
• Quality control of PCR amplification products prior to sequencing or cloning
• Teaching laboratory implementation of core electrophoretic principles under supervised conditions

FAQ

Is the JY-CZ-BL compatible with precast gels?
Yes—its modular inner cassette design accepts commercially available 186 × 205 mm precast polyacrylamide gels from major suppliers.
What power supply specifications are recommended?
A programmable DC power supply capable of delivering up to 500 V and 500 mA is recommended for optimal IEF and high-resolution PAGE performance.
Can the unit be used for native PAGE?
Yes—the absence of denaturing agents in the buffer system and stable temperature control make it suitable for native electrophoresis of oligomeric proteins and protein complexes.
Does the system include a cooling module?
No—the base configuration relies on passive buffer thermal mass; however, a standardized port allows integration with external recirculating chillers for active temperature regulation.
What maintenance is required?
Routine cleaning with deionized water and mild detergent after each use; periodic inspection of electrode contacts and sealing gaskets for integrity and corrosion resistance.